Medium (DMEM/F12)‐containing chitosan hydrogel as adhesive and dressing in autologous skin grafts and accelerator in the healing process

Kiyozumi, Tetsuro; Kanatani, Yasuhiro; Ishihara, Masayuki; Saitoh, Daizoh; Shimizu, Jun; Yura, Hirofumi; Suzuki, Shinya; Okada, Yoshiaki; Kikuchi, Makoto

doi:10.1002/jbm.b.30522

Cited by 50 publications

(35 citation statements)

References 28 publications

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“…This data further confirmed that in addition to hydrolysis, efficient inflammatory cell penetration during the healing process of burn wounds accelerated the degradation of the hydrogel more than control scaffold. Our data agrees with the results of another study suggesting that neutrophils promoted chitosan hydrogel degradation (23). Additionally, recent studies demonstrated that a degradable hydrogel allowed and directed cell growth in vitro (31, 32) compared to nondegradable hydrogels.…”

Section: Resultssupporting

confidence: 83%

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Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing

Sun

Zhang

Shen

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

477

307

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Neovascularization is a critical determinant of wound-healing outcomes for deep burn injuries. We hypothesize that dextran-based hydrogels can serve as instructive scaffolds to promote neovascularization and skin regeneration in third-degree burn wounds. Dextran hydrogels are soft and pliable, offering opportunities to improve the management of burn wound treatment. We first developed a procedure to treat burn wounds on mice with dextran hydrogels. In this procedure, we followed clinical practice of wound excision to remove full-thickness burned skin, and then covered the wound with the dextran hydrogel and a dressing layer. Our procedure allows the hydrogel to remain intact and securely in place during the entire healing period, thus offering opportunities to simplify the management of burn wound treatment. A 3-week comparative study indicated that dextran hydrogel promoted dermal regeneration with complete skin appendages. The hydrogel scaffold facilitated early inflammatory cell infiltration that led to its rapid degradation, promoting the infiltration of angiogenic cells into the healing wounds. Endothelial cells homed into the hydrogel scaffolds to enable neovascularization by day 7, resulting in an increased blood flow significantly greater than treated and untreated controls. By day 21, burn wounds treated with hydrogel developed a mature epithelial structure with hair follicles and sebaceous glands. After 5 weeks of treatment, the hydrogel scaffolds promoted new hair growth and epidermal morphology and thickness similar to normal mouse skin. Collectively, our evidence shows that customized dextran-based hydrogel alone, with no additional growth factors, cytokines, or cells, promoted remarkable neovascularization and skin regeneration and may lead to novel treatments for dermal wounds.

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Section: Resultssupporting

confidence: 83%

“…Many studies of hydrogel-based scaffolds have focused on applications in healing wounds (5,(16)(17)(18)(19)(20)(21). Beyond their utility as scaffolds, hydrogels can also deliver cytokines and growth factors (22,23), antibiotics (20), and cells (24,25) to allow complete skin regeneration.…”

mentioning

confidence: 99%

Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing

Sun

Zhang

Shen

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

477

307

View full text Add to dashboard Cite

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“…19,20) Hydrogels provide a cover on the wound in the absence of injured skin integrity. An ideal gel formulation used for burn healing should be easily applicable and retained for a long time on the wound.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

161

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Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. 1,2) Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. 12) The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits. MATERIALS AND METHODSMaterials Chitosan (MW 250 kDa, deacetylation degree Ն90%, Pronova A/S, Norway; MW 400 kDa, deacetylation degree Ն60%, Fluka, Germany; MW 750 kDa, deacetylation degree Ն75%, Sigma, U.S.A.), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, U.S.A. All other reagents used were of analytical grade.Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300 rpm for 1 h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4°C until experiment.Viscosity Measurement The viscosity of hydrogels was determine...

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“…Both biopolymers have b-glucosidic bonds and very similar structure. Their somewhat unique properties; especially the strong mechanical strength, biocompatibility and thermal stability of cellulose (Nishio 1994;Yamashiki et al 1990), and the wound healing, antibacterial properties of chitosan (Burkatovskaya et al 2006;Jain and Banerjee 2008;Kiyozumi et al 2006;Shepherd et al 1997), as well as their ability of self-assembly into intriguing micro-or nano-sized structures (Qiu and Hu 2013;Wu et al 2016;Zhang et al 2016), provide many options and ideas for functional materials design.…”

Section: Introductionmentioning

confidence: 99%

Spherical nanocomposite particles prepared from mixed cellulose–chitosan solutions

et al. 2016

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Novel cellulose-chitosan nanocomposite particles with spherical shape were successfully prepared via mixing of aqueous biopolymer solutions in three different ways. Macroparticles with diameters in the millimeter range were produced by dripping cellulose dissolved in cold LiOH/urea into acidic chitosan solutions, inducing instant co-regeneration of the biopolymers. Two types of microspheres, chemically crosslinked and non-crosslinked, were prepared by first mixing cellulose and chitosan solutions obtained from freeze thawing in LiOH/KOH/urea. Thereafter epichlorohydrin was applied as crosslinking agent for one of the samples, followed by water-inoil (W/O) emulsification, heat induced sol-gel transition, solvent exchange, washing and freeze-drying. Characterization by X-ray photoelectron spectroscopy, total elemental analysis, and Fourier transform infrared spectroscopy confirmed the prepared particles as being true cellulose-chitosan nanocomposites with different distribution of chitosan from the surface to the core of the particles depending on the preparation method. Field emission scanning electron microscopy and laser diffraction was performed to study the morphology and size distribution of the prepared particles. The morphology was found to vary due to different preparation routes, revealing a core shell structure for macroparticles prepared by dripping, and homogenous nanoporous structure for the microspheres. The non-crosslinked microparticles exhibited a somewhat denser structure than the crosslinked ones, which indicated that crosslinking restricts packing of the chains before and under regeneration. From the obtained volume-weighted size distributions it was found that the crosslinked microspheres had the highest median diameter. The results demonstrate that not only the mixing ratio and distribution of the two biopolymers, but also the morphology and nanocomposite particle diameters are tunable by choosing between the different routes of preparation.

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Medium (DMEM/F12)‐containing chitosan hydrogel as adhesive and dressing in autologous skin grafts and accelerator in the healing process

Cited by 50 publications

References 28 publications

Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing

Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Spherical nanocomposite particles prepared from mixed cellulose–chitosan solutions

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