Medium optimization by orthogonal array and response surface methodology for cholesterol oxidase production by Streptomyces lavendulae NCIM 2499

Abstract

This paper deals with the optimization of culture conditions for the production of cholesterol oxidase (COD) by Streptomyces lavendulae NCIM 2499 using the one-factor-at-a-time method, orthogonal array method and response surface methodology (RSM) approaches. The one-factor-at-a-time method was adopted to investigate the effects of medium components (i.e. carbon and nitrogen) and environmental factors (i.e. initial pH) on biomass growth and COD production. Subsequently, an L12 orthogonal matrix was used to evaluate the significance of glycerol, soyabean meal, malt extract, K2HPO4, MgSO4 and NaCl. The effects of media components were ranked according to their effects on the production of COD as malt extract > soyabean meal > K2HPO4 > NaCl > MgSO4 > glycerol. The subsequent optimization of the four most significant factors viz. malt extract, soyabean meal, K2HPO4 and NaCl, was carried out by employing a central composite rotatable design (CCRD) of RSM. There was a 2.48-fold increase in productivity of COD as compared to the unoptimized media by using these statistical approaches.

Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain

“…RSM optimization has been applied to investigate the interrelationship between different medium components affecting the cell growth and production kinetics of many industrially microbial products [16,20]. Furthermore, the obtained RSM optimization results are in good consistency with those previously reported for medium optimization by RSM for cholesterol oxidase production [37,38]. e authors optimized medium composition by RSM approaches for CO production by S. badius and S. lavendulae NCIM 2499. e authors were able to achieve an increase in volumetric enzyme production by 2.48 and 2 times their initially used production media.…”

Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

“…Voelker and Altaba [37] recorded an increase in the production of cholesterol oxidase by organic nitrogen much greater than inorganic nitrogen. The reason is that organic nitrogen may contain the most types of growth factors and amino acids required for the microbial growth and could be immediately metabolized by cells, thereby supporting the cholesterol oxidase production [29].…”

Cholesterol Oxidase: Source,Properties and Applications.

“…While in contrast Moradpour et al reported that pH of 6.5 supported the maximum production of CHOx [53]. In another investigation by Chauhan et al an initial pH of 7.5 was found to be optimum for S. lavendulae for production of CHOx [54].…”

“…The reason for this may be because organic nitrogen source contains most types of amino acids and growth factors for the growth of bacterium that could be metabolized directly by cells, hence promoting CHOx production [54].…”