Medium optimization for enhanced production of Rifamycin B by Amycolatopsis mediterranei S699: Combining a full factorial design and a statistical approach

Ma, Feng-Xu; Kim, Jung Hun; Kim, Sung‐Bae; Seo, Yang-Gon; Chang, Yong Keun; Hong, Soon-Kwang; Kim, Chang-Joon

doi:10.1016/j.procbio.2008.04.021

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…The significant variables identified from the experiment following a Plackett-Burman design were optimized using the Box-Behnken design and response surface methodology, while the other variables of nonsignificance were fixed at the initial medium level. In developing the regression equation, the relation between the coded values and actual values are described by the following equation: [16] (1)…”

Section: Analytical Techniquesmentioning

confidence: 99%

Media Optimization of Corynebacterium glutamicum for Succinate Production Under Oxygen-Deprived Condition

Jeon

Rajesh²,

Song³

et al. 2013

J. Microbiol. Biotechnol.

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Corynebacterium glutamicum is one of the well-studied industrial strain that is used for the production of nucleotides and amino acids. Recently, it has also been studied as a possible producer of organic acids such as succinic acid, based on its ability to produce organic acids under an oxygen deprivation condition. In this study, we conducted the optimization of medium components for improved succinate production from C. glutamicum under an oxygen deprivation condition by Plackett-Burman design and applied a response surface methodology. A Plackett-Burman design for ten factors such as glucose, ammonium sulfate, magnesium sulfate, potassium phosphate (K2HPO4 and KH2PO4), iron sulfate, manganese sulfate, biotin, thiamine, and sodium bicarbonate was applied to evaluate the effects on succinate production. Glucose, ammonium sulfate, magnesium sulfate, and dipotassium phosphate were found to have significant influence on succinate production, and the optimal concentrations of these four factors were sequentially investigated by the response surface methodology using a Box-Behnken design. The optimal medium components obtained for achieving maximum concentration of succinic acid were as follows: glucose 10 g/l, magnesium sulfate 0.5 g/l, dipotassium phosphate (K2HPO4) 0.75 g/l, potassium dihydrogen phosphate (KH2PO4) 0.5 g/l, iron sulfate 6 mg/l, manganese sulfate 4.2 mg/l, biotin 0.2 mg/l, thiamine 0.2 mg/l, and sodium bicarbonate 100 mM. The parameters that differed from a normal BT medium were glucose changed from 40 g/l to 10 g/l, dipotassium phosphate (K2HPO4) 0.5 g/l changed to 0.75 g/l, and ammonium sulfate ((NH4)2SO4) 7 g/l changed to 0 g/l. Under these conditions, the final succinic acid concentration was 16.3 mM, which is about 1.46 fold higher than the original medium (11.1 mM) at 24 h. This work showed the improvement of succinate production by a simple change of media components deduced from sequential optimization.

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Section: Analytical Techniquesmentioning

confidence: 99%

Media Optimization of Corynebacterium glutamicum for Succinate Production Under Oxygen-Deprived Condition

Jeon

Rajesh²,

Song³

et al. 2013

J. Microbiol. Biotechnol.

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“…The first model experiment of the Na matrix effect was a 2 n full-factorial design which is an efficient mathematical approach to optimize responses [44][45][46]. The two applied factors were the concentration of the Na matrix and the measured elements, while their levels as factorial points were abbreviated by MIN and MAX, as well as an additional CENT was applied for the centrum concentration (as indicated in Table 1.).…”

Section: Discussionmentioning

confidence: 99%

Elemental Analysis of Human Blood Serum by Microwave Plasma—Investigation of the Matrix Effects Caused by Sodium Using Model Solutions

Baranyai

Tóth

Fábián

2019

Biol Trace Elem Res

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Human blood is a complex sample matrix when elemental analysis is considered. In this study, the effects of Na, a natural component of serum samples, was investigated in the quantitative determination of Ca, K, Mg, Cu, Zn, and Fe by microwave plasma atomic emission spectrometry. The robustness of the microwave plasma was tested by evaluating MgII 280.271 nm/MgI 285.213 nm by varying two adjustable operating parameters, the read time, and the nebulizer pressure. The read time has no influence on the robustness while the MgII/MgI ratio decreased when the nebulizer pressure was increased during the analysis. The threshold concentrations of the interfering Na were determined at the analytical lines used for the measurement of other elements. The matrix effect of the commercially available microwave plasma was studied by a series of model experiments with human blood. The increasing concentration of Na in the matrix within the normal ranges reported for blood serum increased the intensities of the measured atomic lines. According to a factorial design-where two applied factors were the concentration of Na matrix and the measured elements as well as their levels were considered as factorial points-it was found that the Na concentration in a serum sample after acid digestion and 10 times dilution affected the intensity values of the measured elements. For Ca, Cu, and Fe, a statistically significant effect was observed, while for Zn, Mg, and K, an interaction effect was also found. However, after calculating the percentage errors caused by the shift, the relative difference was observed to be quite small (< 10%).

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“…Various scientific groups have achieved rifamycin B production up to 1.2 g/L using A. mediterranei S699 (Ma et al 2008), 2.45 g/L by A. mediterranei VA18 , 3.6 g/L by A. mediterranei (Bapat et al 2006), 4.4 g/L by Nocardia RSP-3 (Mahalaxmi et al 2008), 4.6 g/L A. mediterranei MV35R (Murali Krishna et al 2000) and 5.12 g/L by A. mediterranei RSP-3 (Mahalaxmi et al 2009) under optimized submerged fermentation conditions. It is to be noted that the quantity of rifamycin B on conversion to rifamycin SV gets reduced further as in the case with some of the abovecited research studies.…”

Section: Organic Nitrogen Sourcesmentioning

confidence: 99%

“…Optimization of antibiotic production necessitates the study of different physical and physiological factors affecting their yields of the end product. This hitherto is done by taking into concern the optimal fermentation conditions which are related to type of strain being used and its biochemistry Bapat et al 2005;Ma et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei

Nagavalli

Ponamgi

Girijashankar

et al. 2015

Appl Microbiol Biotechnol

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Rifamycin is a broad-spectrum antimicrobial drug produced commercially by submerged fermentation where the yields are far less in comparison to its demand in human drug therapy. Addressing the need, sequential mutational strain improvement was carried using UV and EtBr that resulted in improved strain yielding rifamycin SV up to 4.32 g/L. Further optimization of six important fermentation factors was followed which include temperature, agitation, inoculum level, period of fermentation, inorganic nitrogen source and amino acids. For the first time, we report a maximum yield of 5.32 g/L of rifamycin SV. Among the amino acids, proline known for its slowest assimilation by Amycolatopsis mediterranei produced the highest improvement in antibiotic yields. Following mutational strain improvement and process optimization, a total of 3.8-fold increase in antibiotic titre was achieved. Following a conventional procedure of mutational strain improvement, highest yield of rifamycin SV was reported by optimizing submerged fermentation process.

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Medium optimization for enhanced production of Rifamycin B by Amycolatopsis mediterranei S699: Combining a full factorial design and a statistical approach

Cited by 15 publications

References 38 publications

Media Optimization of Corynebacterium glutamicum for Succinate Production Under Oxygen-Deprived Condition

Media Optimization of Corynebacterium glutamicum for Succinate Production Under Oxygen-Deprived Condition

Elemental Analysis of Human Blood Serum by Microwave Plasma—Investigation of the Matrix Effects Caused by Sodium Using Model Solutions

Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei

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