Medizinische und �konomische Aspekte der Zentrumsbildung in der Wundbehandlung

Debus, Eike Sebastian; Winkler, Markus; Larena-Avellaneda, A.; Bültemann, Anke; Daum, H.; Lingenfelder, Michael; Schulenburg, B.; Gross-Fengels, W.

doi:10.1007/s00772-003-0319-y

Cited by 13 publications

(8 citation statements)

References 9 publications

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“…These biofilm communities are known to be more resistant to physical and chemical stresses than planktonic cells and can cause major economic loss within industrial sectors (7). Moreover, more than 4 million people in Germany suffer from chronic wounds, which are often caused by resistant biofilm-forming pathogens (8). The treatment costs for these patients amount to up to 4 billion euros per year in Germany alone (9).…”

mentioning

confidence: 99%

Nonthermal Plasma Jet Treatment Negatively Affects the Viability and Structure of Candida albicans SC5314 Biofilms

Handorf

Weihe

Bekeschus

et al. 2018

Appl Environ Microbiol

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Microorganisms are predominantly organized in biofilms, where cells live in dense communities and are more resistant to external stresses than are their planktonic counterparts. With experiments, the susceptibility of biofilms to a nonthermal plasma treatment (plasma source, kINPen09) in terms of growth, survival, and cell viability was investigated. strain SC5314 (ATCC MYA-2876) was plasma treated for different time periods (30 s, 60 s, 120 s, 180 s, 300 s). The results of the experiments, encompassing CFU, fluorescence Live/Dead, and 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT) assays, revealed a negative influence of the plasma treatment on the proliferation ability, vitality, and metabolism of biofilms, respectively. Morphological analysis of plasma-treated biofilms using atomic force microscopy supported the indications for lethal plasma effects concomitant with membrane disruptions and the loss of intracellular fluid. Yielding controversial results compared to those of other publications, fluorescence and confocal laser scanning microscopic inspection of plasma-treated biofilms indicated that an inactivation of cells appeared mainly on the bottom of the biofilms. If this inactivation leads to a detachment of the biofilms from the overgrown surface, it might offer completely new approaches in the plasma treatment of biofilms. Because of plasma's biochemical-mechanical mode of action, resistance of microbial cells against plasma is unknown at this state of research. Microbial communities are an increasing problem in medicine but also in industry. Thus, an efficient and rapid removal of biofilms is becoming increasingly important. With the aid of the kINPen09, a radiofrequency plasma jet (RFPJ) instrument, decisive new findings on the effects of plasma on biofilms were obtained. This work showed that the inactivation of biofilms takes place mainly on the bottom, which in turn offers new possibilities for the removal of biofilms by other strategies, e.g., mechanical treatment. This result demonstrated that nonthermal atmospheric pressure plasma is well suited for biofilm decontamination.

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mentioning

confidence: 99%

Nonthermal Plasma Jet Treatment Negatively Affects the Viability and Structure of Candida albicans SC5314 Biofilms

Handorf

Weihe

Bekeschus

et al. 2018

Appl Environ Microbiol

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“…Auf dem Boden verbesserter Behandlungsmöglichkeiten, einer Zunahme gefäßkranker Patienten sowie übergeordneter Forderungen nach interdisziplinä-rer Kooperation wurden auch in Deutschland Gefäßzentren gebildet [4,11].…”

Section: Diskussionunclassified

Ergebnisse der Zertifizierung von Gefäßzentren durch die Deutsche Gesellschaft für Gefäßchirurgie

Eckstein

Niedermeier

Noppeney

et al. 2006

Dtsch med Wochenschr

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Hintergrund und Fragestellung: Die Fortschritte im Bereich der konventionellen Gefäßchirurgie, der endovaskulären Verfahren und der konservativen Therapie erfordern eine enge interdisziplinäre Kooperation. Der Terminus Gefäßzentrum signalisiert gegenüber Patienten und Zuweisern eine optimale fachliche und organisatorische Kompetenz. Zur Sicherstellung der inhaltlichen und strukturellen Qualität hat die Deutsche Gesellschaft für Gefäßchirurgie einen Kriterienkatalog zur Zertifizierung interdisziplinärer Gefäßzentren erarbeitet. Essentiell sind dabei die jederzeit verfügbare Notfalltherapie, Interdisziplinarität und adäquate Fall-und Leistungszahlen. Methoden: Zwischen Juli 2002 und Dezember 2005 wurden 73 Anträge eingereicht. 54 Gefäßzentren wurden nach Audit für 3 Jahre zertifiziert, davon je ein Zentrum in Österreich und der Schweiz. 13 Zentren wurden abgelehnt (17,8%), 6 Anträge werden bearbeitet. Für diese Analyse wurden die Daten von 52 bundesdeutschen zertifizierten Gefäßzentren ausgewertet. Ergebnisse: In den Zentren werden im Median 1149 Patienten/Jahr stationär (davon 12% als Notfall) und 2159 Patienten/Jahr ambulant be-

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“…These biofilm communities are known to be more resistant to physical and chemical stresses compared to planktonic cells, and can cause major economic loss within industrial sectors (4). Moreover, more than four million people in Germany suffer from chronic wounds, which are often caused by resistant biofilm-forming pathogens (5). The treatment costs of these patients amount up to four billion euro per year only in Germany (6).…”

Section: Introductionmentioning

confidence: 99%

Effects on cell viability, growth and morphology ofC. albicansSC5314 biofilms after kINPen®09 plasma treatment

Handorf

Weihe

Bekeschus

et al. 2018

Preprint

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Medizinische und �konomische Aspekte der Zentrumsbildung in der Wundbehandlung

Cited by 13 publications

References 9 publications

Nonthermal Plasma Jet Treatment Negatively Affects the Viability and Structure of Candida albicans SC5314 Biofilms

Nonthermal Plasma Jet Treatment Negatively Affects the Viability and Structure of Candida albicans SC5314 Biofilms

Ergebnisse der Zertifizierung von Gefäßzentren durch die Deutsche Gesellschaft für Gefäßchirurgie

Effects on cell viability, growth and morphology ofC. albicansSC5314 biofilms after kINPen®09 plasma treatment

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