Medullary neurons with projections to lamina X of the rat as demonstrated by retrograde labeling after HRP microelectrophoresis

Du, Huan-Ji

doi:10.1016/0006-8993(89)90124-8

Cited by 18 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following PRV injection into the urethra, labeled cells were consistently observed in the nucleus raphe magnus (RMg), A5, the KF nucleus which is part of the A7 noradrenergic cell group, and nucleus subcoeruleus. The RMg projects to multiple levels of the spinal cord including the IML and lamina X of the thoracic and lumbar spinal cord (Basbaum et al, 1976;Loewy, 1981;Du, 1989). In addition, fibers from the RMg terminate at all spinal cord levels in the superficial dorsal horn (laminae 1-11) and in deeper layers of the dorsal horn (laminae V-Vl) (Basbaum et al, 1976).…”

Section: Discussionmentioning

confidence: 99%

Transneuronal labeling of neurons in the adult rat brainstem and spinal cord after injection of pseudorabies virus into the urethra

Vizzard¹,

Erickson²,

Card³

et al. 1995

J of Comparative Neurology

173

110

View full text Add to dashboard Cite

Transneuronal tracing techniques were used to identify sites in the central nervous system involved in the neural control of urethral function. The distribution of virus-infected neurons was examined in the spinal cord and brainstem at various intervals (56-96 hours) following pseudorabies virus (PRV) injection into the urethra. In the lumbosacral (L6-S1) spinal cord at 56 hours, neurons containing PRV immunoreactivity (PRV-IR) were located in the region of the sacral parasympathetic nucleus (SPN), around the central canal, and in the dorsal commissure. Some animals also exhibited PRV-IR in cells in the L6 dorsolateral motor nucleus. At longer survival times (72-96 hours), PRV-IR cells were observed in the superficial and deeper laminae of the dorsal horn, and increased numbers of PRV-IR cells were consistently detected in the region of the SPN, around the central canal, and in the dorsal commissure. PRV-IR fiber-like staining also occurred along the lateral edge of the dorsal horn extending from Lissauer's tract to the region of the SPN. In rostral lumbar segments (L1-L2), PRV-IR cells were located in the region of the dorsal commissure and the intermediolateral cell nucleus (IML), around the central canal, and in the dorsal horn. After 72-84 hours, PRV-IR cells were also noted at more rostral levels of the neuraxis including the medulla, pons, midbrain, and diencephalon. At 72 hours, PRV-IR cells were consistently observed in Barrington's nucleus (pontine micturition center), nucleus raphe magnus (RMg), parapyramidal reticular formation, and the A5 and A7 regions. At 78-84 hours, additional regions exhibited PRV-IR cells, including the periaqueductal gray, locus coeruleus, the dorsal and ventral subcoeruleus alpha, and the red nucleus. A few cells were also located in the lateral hypothalamic area. This distribution of PRV-labeled cells in the spinal cord and brainstem is similar in many respects to the distribution of cells labeled in previous studies by PRV injection into the urinary bladder. This overlap of urethra and bladder neurons is consistent with the results of physiological experiments indicating a close coordination between the central nervous control of bladder and urethral activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Transneuronal labeling of neurons in the adult rat brainstem and spinal cord after injection of pseudorabies virus into the urethra

Vizzard¹,

Erickson²,

Card³

et al. 1995

J of Comparative Neurology

173

110

View full text Add to dashboard Cite

show abstract

“…In addition to the superficial dorsal horn, the deep dorsal horn, the intermediolateral cell column, and the central canal region also receive dense innervation from RM neurons (Antal et al 1996, Bullitt & Light 1989, Jones & Light 1990. Retrograde transport studies confirm that neurons in RM and NRMC project to the thoracic intermediolateral cell column, the central canal region, and even to the ventral horn (Du 1989, Masson et al 1991, Sasek et al 1990. Unfortunately, these studies cannot distinguish between projections to different cell types present in the same region, such as ventral horn motoneurons and neighboring interneurons.…”

Section: Descending Projections Of the Medullary Raphe And Ventromedimentioning

confidence: 90%

“…The relative preponderance of RM and NRMC cell projections to the dorsal, intermediate, and ventral horns is unclear and is a difficult question to address experimentally. It is notable, in this regard, that no RM or NRMC cells were labeled after very small tracer injections into any portion of the dorsal horn, although cells were labeled after similarly sized injections into the central canal region, an area that contains pre-preganglionic as well as premotoneuronal interneurons (Du 1989). …”

Section: Descending Projections Of the Medullary Raphe And Ventromedimentioning

confidence: 95%

Contributions of the Medullary Raphe and Ventromedial Reticular Region to Pain Modulation and Other Homeostatic Functions

Mason

2001

Annu. Rev. Neurosci.

224

154

View full text Add to dashboard Cite

The raphe magnus is part of an interrelated region of medullary raphe and ventromedial reticular nuclei that project to all areas of the spinal gray. Activation of raphe and reticular neurons evokes modulatory effects in sensory, autonomic, and motor spinal processes. Two physiological types of nonserotonergic cells are observed in the medullary raphe and are thought to modulate spinal pain processing in opposing directions. Recent evidence suggests that these cells may modulate stimulus-evoked arousal or alerting rather than pain-evoked withdrawals. Nonserotonergic cells are also likely to modulate spinal autonomic and motor circuits involved in thermoregulation and sexual function. Medullary serotonergic cells have state-dependent discharge and are likely to contribute to the modulation of pain processing, thermoregulation, and sexual function in the spinal cord. The medullary raphe and ventromedial reticular region may set sensory, autonomic, and motor spinal circuits into configurations that are appropriate to the current behavioral state.

show abstract

“…Other regions that were consistently labelled with PRV included the nGi, the raphe magnus, and the raphe pallidus. The raphe magnus and raphe pallidus project to multiple levels of the spinal cord, including the lumbar IML and the medial gray (Loewy, 1981;Du, 1989;Marson and McKenna, 1992). Stimulation of the raphe magnus and the nGi inhibits the spontaneously contracting bladder (McMahon and Spillane, 1982).…”

Section: Brainstemmentioning

confidence: 99%

Identification of central nervous system neurons that innervate the bladder body, bladder base, or external urethral sphincter of female rats: A transneuronal tracing study using pseudorabies virus

Marson

1997

J. Comp. Neurol.

131

View full text Add to dashboard Cite

Transneuronal tracing techniques were used to identify the putative spinal and brainstem neurons involved in continence and voiding in the female rat. Pseudorabies virus, Bartha's K strain, was injected into either the external urethral sphincter, the bladder base, or the bladder body. After 3-5 days, the rats were perfused with fixative, and virus-labelled cells were identified by using immunohistochemistry. External urethral sphincter (EUS) injections resulted in labelling of pudendal motoneurons in the dorsolateral nucleus of L6. Putative spinal interneurons were found in the medial cord from T13 to S1 and in the lateral gray of T13-L2 and L5-S1. After both bladder base and bladder body injections, the majority of pseudorabies virus-labelled cells were found in the lateral gray and medial cord of L6-S1. A number of those found in the intermediolateral cell column resembled the parasympathetic preganglionic neurons; the remaining neurons in the lateral and medial gray were presumed to be interneurons. Very few pseudorabies virus-labelled cells were found rostral to T10. In the brainstem, transneuronally labelled cells were found in the parapyramidal medullary reticular formation, Barrington's nucleus, raphe magnus, raphe pallidus, subcoeruleus pars alpha, locus coeruleus, the A5 noradrenergic cell group, and ventromedial periaqueductal gray after all injection sites. Pseudorabies virus-labelled cells were also seen in the forebrain following the longest survival times; areas consistently labelled included the lateral hypothalamus, the parvocellular region of the paraventricular nucleus, and the medial preoptic area. These studies indicate that there is a substantial overlap of central nervous system neurons that innervate the EUS and the bladder in the female.

show abstract

Medullary neurons with projections to lamina X of the rat as demonstrated by retrograde labeling after HRP microelectrophoresis

Cited by 18 publications

References 28 publications

Transneuronal labeling of neurons in the adult rat brainstem and spinal cord after injection of pseudorabies virus into the urethra

Transneuronal labeling of neurons in the adult rat brainstem and spinal cord after injection of pseudorabies virus into the urethra

Contributions of the Medullary Raphe and Ventromedial Reticular Region to Pain Modulation and Other Homeostatic Functions

Identification of central nervous system neurons that innervate the bladder body, bladder base, or external urethral sphincter of female rats: A transneuronal tracing study using pseudorabies virus

Contact Info

Product

Resources

About