Megakaryoblastic differentiation of proerythroblastic K562 cell-line cells

Tetteroo, P. A. T.; Massaro, Fabiana C.; Mulder, A. H. L.; Gelder, R.S. van; Borne, A. E. G. K. Von Dem

doi:10.1016/0145-2126(84)90143-7

Cited by 165 publications

(110 citation statements)

References 22 publications

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“…The phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA), although originally described as a tumor promoter, has been shown to induce differentiation in a variety of hematopoietic cells (1,2,6-8, 11,13,14,17-20). TPA has been reported to inhibit K-562 cell growth and to drive K-562 cells toward megakaryoblastic differentiation (5,22). While studying the effect of TPA on K-562 cell cycle regulation, we found that, although TPA completely inhibited K-562 cell proliferation, 3H-thymidine incorporation by K-562 cells was actually markedly increased following TPA treatment.…”

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confidence: 63%

Phorbol ester enhances deoxynucleoside incorporation while inhibiting proliferation of K‐562 cells

1985

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Recently a new technique has been developed to study bromodeoxyuridine incorporation into DNA as a function of the cell cycle phase distribution. We have made the observation that treatment of the hematopoietic cell line K-562 results in a complete and immediate cessation of cell growth, although tritiated thymidine incorporation is markedly elevated for as long as 5 days. We have made use of that newly described technique to study this phenomenon.

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confidence: 63%

Phorbol ester enhances deoxynucleoside incorporation while inhibiting proliferation of K‐562 cells

1985

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“…The strategy of Wilks (13) was used to amplify tyrosine kinase sequences after reverse transcription of polyadenylylated RNA of K-562 cells. As a source of cDNA for sequence amplification, we also used tumor promoter-treated K-562 cells, which have been shown to undergo megakaryoblastoid differentiation (11,12). Two degenerate oligonucleotides corresponding to the IHRDL and DVWSFG (singleletter code) conserved amino acid sequences and with flanking restriction enzyme-cleavage sites were used for the amplification.…”

Section: Resultsmentioning

confidence: 99%

“…K-562 is a chronic myelogenous leukemia cell line that has a bipotential differentiation capacity: the cells have features of erythroleukemia (8,9), but treatment with tumor promoters induces them to differentiate into the megakaryoblastoid direction (10)(11)(12). We were interested in tyrosine kinases expressed in these cells because of their potential role in platelet/erythrocyte-specitic tyrosine phosphorylation.…”

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confidence: 99%

Putative tyrosine kinases expressed in K-562 human leukemia cells.

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Mäkelä

Alitalo

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

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Tyrosine phosphorylation is important in the transmission of growth and differentiation signals; known tyrosine kinases include several oncoproteins and growth factor receptors. Interestingly, some differentiated cell types, such as erythrocytes and platelets contain high amounts of phosphotyrosine. We analyzed tyrosine kinases expressed in the K-562 chronic myelogenous leukemia cell line, which has a bipotential erythroid and megakaryoblastoid differentiation capacity.

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“…10 This differentiation process is characterized by changes in cell morphology and adhesive properties, cell growth arrest, endomitosis and expression of markers associated with megakaryocytes. [10][11][12][13][14][15] The signaling cascade that leads to PMA-induced cell cycle arrest, polyploidy and differentiation of K562 cells has been partially described and the role of PKC in the megakaryocyte differentiation process has been established. 16 Studies carried out with K562 cells revealed that PMA induces the translocation of specific isoforms of protein kinase C (PKC) to particulate fractions (plasma and nuclear membranes) with subsequent reduction in the levels of the ␤11 PKC isoform and an increase in the ␣ PKC isoform.…”

Section: Introductionmentioning

confidence: 99%