Megasporogenesis, microsporogenesis, and female and male gametophyte development in Ziziphus jujuba Mill

Guo, Yifeng; Li, Xiang; Huang, Feiyi; Pang, Xiaoming; Li, Yingyue

doi:10.1007/s00709-019-01395-x

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…To observe mitosis in zygotes, the preserved flower buds were studied by the improved paraffin section technique described by Guo et al (2019) . Sections were observed and photographed under a microscope (BX51; Olympus) equipped with a CCD camera (DP70; Olympus).…”

Section: Methodsmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

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Improvements in plant growth can bring great benefits to the forest industry. Eucalyptus urophylla is an important plantation species worldwide, and given that ploidy increases are often associated with plant phenotype changes, it was reasoned that its polyploidization may have good prospects and great significance toward its cultivation. In this study, the zygotic development period of E. urophylla was observed through paraffin sections, and a correlation between the development time of flower buds after pollination and the zygotic development period was established. On this basis, it was determined that the 25th day after pollination was the appropriate time for a high temperature to induce zygotic chromosome doubling. Then tetraploid E. urophylla was successfully obtained for the first time through zygotic chromosome doubling induced by high temperature, and the appropriate conditions were treating flower branches at 44°C for 6 h. The characterization of tetraploid E. urophylla was performed. Chromosome duplication brought about slower growing trees with thicker leaves, larger cells, higher net photosynthetic rates, and a higher content of certain secondary metabolites. Additionally, the molecular mechanisms for the variation in the tetraploid’s characteristics were studied. The qRT-PCR results showed that genes mediating the tetraploid characteristics showed the same change trend as those of the characteristics, which verified that tetraploid trait variation was mainly caused by gene expression changes. Furthermore, although the tetraploid had no growth advantage compared with the diploid, it can provide important germplasm resources for future breeding, especially for the creation of triploids.

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Section: Methodsmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

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“…Differences were considered statistically significant at p < 0.05. length and width were measured using a stereomicroscope and the tracheid length-width ratio was calculated. The buds, leaves, and stems from N′ and V′ were studied using an improved paraffin section technique (Ruzin, 1999;Guo et al, 2019). The samples were dehydrated in ethanol solutions of different concentrations and then treated with dimethyl benzene and embedded in paraffin.…”

Section: Measurement Of Growth Traitsmentioning

confidence: 99%

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Cai

Zhou

et al. 2021

Front. Plant Sci.

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Larix olgensis is a tall deciduous tree species that has many applications in the wood fiber industry. Bud mutations are somatic mutations in plants and are considered an ideal material to identify and describe the molecular mechanism of plant mutation. However, the molecular regulatory mechanisms of bud mutations in L. olgensis remain unknown. In this study, dwarfed (or stunted), short-leaved, and multi-branched mutants of L. olgensis were found and utilized to identify crucial genes and regulatory networks controlling the multiple branch structure of L. olgensis. The physiological data showed that the branch number, bud number, fresh and dry weight, tracheid length, tracheid length-width ratio, inner tracheid diameter, and epidermal cell area of mutant plants were higher than that of wild-type plants. Hormone concentration measurements found that auxin, gibberellin, and abscisic acid in the mutant leaves were higher than that in wild-type plants. Moreover, the transcriptome sequencing of all samples using the Illumina Hiseq sequencing platform. Transcriptome analysis identified, respectively, 632, 157, and 199 differentially expressed genes (DEGs) in buds, leaves, and stems between mutant plants and wild type. DEGs were found to be involved in cell division and differentiation, shoot apical meristem activity, plant hormone biosynthesis, and sugar metabolism. Furthermore, bZIP, WRKY, and AP2/ERF family transcription factors play a role in bud formation. This study provides new insights into the molecular mechanisms of L. olgensis bud and branch formation and establishes a fundamental understanding of the breeding of new varieties in L. olgensis.

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“…Male sterility was mainly due to the abnormal tapeutm behavior, leading to produce some abnormal microspores or empty anthers, or affecting the anther dehiscence and subsequent fertilization process, as Guo et al [ 26 ] found in the study of jujube. In different plant species, tapetum degeneration usually began at the tetrad stage or the later mononuclear pollen grain stage [ 18 , 23 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Sporogenesis, gametophyte development and embryogenesis in Glehnia littoralis

Zhou

Zhang

et al. 2023

BMC Plant Biol

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Background Glehnia littoralis is an economic herb with both medicinal and edible uses. It also has important ecological value and special phylogenetic status as it is a monotypic genus species distributing around beach. Little information on its reproductive biology has been reported so far, which has hindered conservation and application of this species. In this study, we observed morphological changes from buds emergence to seeds formation and internal changes during sporogenesis, gametophyte development and embryo and endosperm development of G. littoralis using paraffin-embedded-sectioning and stereo microscope. Results The results showed that the stages of internal development events of G. littoralis corresponded to obvious external morphological changes, most of developmental features were consistent with other Apiaceae species. The development of male and female gametophytes was not synchronized in the same flower, however, exhibited temporal overlap. From mid-late April to mid-May, the anther primordial and ovule primordial developed into the trinucleate pollen grain and eight-nuclear embryo sac, respectively. From late-May to mid-July, the zygote developed into mature embryo. In addition, some defects in gynoecium or ovule development and abnormal embryo and endosperm development were found. We induced that the possible causes of abortion in G. littoralis were as follows: nutrient limitation, poor pollination and fertilization, and bad weather. Conclusions This study revealed the whole process and morphological characteristics of the development of reproductive organ in G. littoralis, which not only provided important data for the study of systematic and conservation biology, but also provided a theoretical basis for cross breeding.

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Megasporogenesis, microsporogenesis, and female and male gametophyte development in Ziziphus jujuba Mill

Cited by 5 publications

References 33 publications

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Sporogenesis, gametophyte development and embryogenesis in Glehnia littoralis

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