Meiosis and ascospore development in Cochliobolus heterostrophus

Raju, Namboori B.

doi:10.1016/j.fgb.2007.08.007

Cited by 9 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…we could observe all stages of asci with a single nucleus, as well as 2-nucleus, 4-nucleus, 8-nucleus, 16-nucleus, and multinucleate ascospores (Fig. 6A-F), as Raju (2008) reported previously. In crosses of Δsec5 strains with the albino strain, meiotic stages of asci were found (Fig.…”

Section: Sec5 Is Essential For Ascospore Formationsupporting

confidence: 90%

An exocyst component, Sec5, is essential for ascospore formation in <i>Bipolaris maydis</i>

et al. 2021

View full text Add to dashboard Cite

In this study, we identified Sec5 in Bipolaris maydis, a homologue of Sec5 in Saccharomyces cerevisiae and a possible exocyst component of the fungus. To examine how Sec5 affects the life cycle of B. maydis, we generated null mutant strains of the gene (Δsec5). The Δsec5 strains showed a strong reduction in hyphal growth and a slight reduction in pathogenicity. In sexual reproduction, they possessed the ability to develop pseudothecia. However, all ascospores were aborted in any of the asci obtained from crosses between Δsec5 and the wild-type. Our cytological study revealed that the abortion was caused by impairments of the post-meiotic stages in ascospore development, where ascospore delimitation and young spore elongation occur.

show abstract

Section: Sec5 Is Essential For Ascospore Formationsupporting

confidence: 90%

An exocyst component, Sec5, is essential for ascospore formation in <i>Bipolaris maydis</i>

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The crozier development and ascus formation in G. zeae are nearly identical to those of the well-documented heterothallic species of Neurospora and Cochliobolus (Raju 1980;Raju 2008). The seconddivision spindles of G. zeae overlap as in N. tetrasperma, but spore delimitation is similar to that of Neurospora crassa (Raju 2009).…”

Section: Resultssupporting

confidence: 62%

Meiotic silencing in the homothallic fungus Gibberella zeae

et al. 2011

Self Cite

View full text Add to dashboard Cite

“…Early ascus development and nuclear divisions in C. heterostrophus resemble those of N. crassa. However, the two fungi differ in several important details owing to differences in ascus and ascospore shape, spindle pole body behaviour during spore delimitation and ascospore development (Raju 2008a). The two spindles at meiosis II, and the four spindles at the postmeiotic mitosis are aligned irregularly, unlike the tandem or ladder rung-like orientation of spindles in N. crassa.…”

Section: Sexual Biology Of Other Ascomycotamentioning

confidence: 99%

“…In crosses of Sk K x Sk S , a vast majority of asci contain 4 or fewer mature ascospores (Sk K ); the 4 ascospores that carry Sk S nuclei abort shortly after spore delimitation (Raju 1994). An illustrated account of ascus and ascospore development in C. heterostrophus is now available (Raju 2008a).…”

Section: Sexual Biology Of Other Ascomycotamentioning

confidence: 99%

Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Raju

2009

J Biosci

Self Cite

View full text Add to dashboard Cite

Dodge's early work (1927-1940) on Neurospora genetics and sexual biology inspired Beadle and Tatum at Stanford to use N.crassa for their landmark discovery that genes specify enzymes. Neurospora has since become a model organism for numerous genetic, cytogenetic, biochemical, molecular and population biology studies. Neurospora is haploid in the vegetative phase with a transient diploid sexual phase. Its meiotic cells (asci) are large, allowing easy examination of dividing nuclei and chromosomes under a light microscope. The haploid meiotic products are themselves the sexual progeny that grow into vegetative cultures, thus avoiding the cumbersome testcrosses and complex dominance -recessive relationships, as in diploid organisms.The Perkins'laboratory at Stanford (1949-2007) played a pivotal role in advancing our knowledge of Neurospora genetics, sexual biology, cytogenetics and population biology. Since 1974, I have taken advantage of various chromosome-staining methods to examine ascus and ascospore development in wild type and in numerous mutant strains. In addition,I have used GFP-tagged genes to visualize the expression or silencing of unpaired genes in a post-transcriptional gene silencing process (meiotic silencing by unpaired DNA) that operates specifically during meiosis. The genome of N. crassa contains over 10 000 protein- coding genes. Gene knockouts or mutations in specific sequences may now be readily correlated with the observed cytological defects in the sexual stage, thus advancing our molecular understanding of complex processes during ascus and ascospore development.

show abstract

Meiosis and ascospore development in Cochliobolus heterostrophus

Cited by 9 publications

References 35 publications

An exocyst component, Sec5, is essential for ascospore formation in <i>Bipolaris maydis</i>

An exocyst component, Sec5, is essential for ascospore formation in <i>Bipolaris maydis</i>

Meiotic silencing in the homothallic fungus Gibberella zeae

Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Contact Info

Product

Resources

About