Meiotic Gene Evolution: Can You Teach a New Dog New Tricks?

Lloyd, A. B.; Ranoux, Marion; Vautrin, Sonia; Glover, Natasha; Fourment, Joëlle; Charif, Delphine; Choulet, Frédéric; Lassalle, Gilles; Marande, William; Tran, Joseph; Granier, Fabienne; Pingault, Lise; Remay, Arnaud; Marquis, Catherine; Belcram, Harry; Chalhoub, Boulos; Feuillet, Catherine; Bergès, Hélène; Sourdille, Pierre; Jenczewski, Eric

doi:10.1093/molbev/msu119

Cited by 54 publications

(98 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As can be seen in Figure 3, duplicate retention subsequent to WGD follows an L-shaped curve that can be approximated by a power-law function (see Methods), confirming common expectations that gene loss subsequent to WGD is initially fast and then slows down. A similar power-law pattern was recently also observed in a genome-wide analysis of duplicate retention following WGD for a more restricted set of genomes (Lloyd et al, 2014). For ease of interpretation, we grouped the WGD events into three different sets according to the overall time frame during which the WGD event occurred.…”

Section: Homoeologs Are Quickly Lost Following Wgdsupporting

confidence: 62%

See 1 more Smart Citation

Gene Duplicability of Core Genes Is Highly Consistent across All Angiosperms

et al. 2016

View full text Add to dashboard Cite

Section: Homoeologs Are Quickly Lost Following Wgdsupporting

confidence: 62%

“…The large number of WGD events in this study and their different ages (Figure 1) provide an excellent case to study duplicate retention following WGD (Lloyd et al, 2014).…”

Section: Homoeologs Are Quickly Lost Following Wgdmentioning

confidence: 99%

Gene Duplicability of Core Genes Is Highly Consistent across All Angiosperms

et al. 2016

View full text Add to dashboard Cite

“…For promoter and terminator regions, we used available data when it was already defined or we considered 2-kb upstream or downstream after UTR regions. Concerning IWGSC CSS (International Wheat Genome Sequencing Consortium 2014), we used the Munich Information Center for Protein Sequences (MIPS) annotation as well as new transcript regions identified by RNA-sequencing (RNA-seq) mapping (Lloyd et al 2014;see below). TEs associated to IWGSC CSS contigs were identified using RepeatMasker and ClariTE (Daron et al 2014).…”

Section: Sequence Information and Analysesmentioning

confidence: 99%

“…A total of 656,290,406 RNA-seq reads from four meiotic stages (latent/leptotene, zygotene/pachytene, diplotene/diakinesis, and metaphase I) (Lloyd et al 2014; data publicly available at http://wheat-urgi.versailles.inra.fr/Seq-Repository/Expression) with two replicates were mapped on 86,710 CSS contigs from IWGSC (International Wheat Genome Sequencing Consortium 2014) used as a reference with TopHat2 (version 2.0.13) with zero mismatches (-m 0 -N 0 options). Mapped reads were then filtered using SAMTools and only those mapped with a minimum mapping quality of 30 were kept.…”

Section: Rna-seq Mappingmentioning

confidence: 99%

High-Resolution Mapping of Crossover Events in the Hexaploid Wheat Genome Suggests a Universal Recombination Mechanism

et al. 2017

Self Cite

View full text Add to dashboard Cite

During meiosis, crossovers (COs) create new allele associations by reciprocal exchange of DNA. In bread wheat (Triticum aestivum L.), COs are mostly limited to subtelomeric regions of chromosomes, resulting in a substantial loss of breeding efficiency in the proximal regions, though these regions carry 60-70% of the genes. Identifying sequence and/or chromosome features affecting recombination occurrence is thus relevant to improve and drive recombination. Using the recent release of a reference sequence of chromosome 3B and of the draft assemblies of the 20 other wheat chromosomes, we performed fine-scale mapping of COs and revealed that 82% of COs located in the distal ends of chromosome 3B representing 19% of the chromosome length. We used 774 SNPs to genotype 180 varieties representative of the Asian and European genetic pools and a segregating population of 1270 F 6 lines. We observed a common location for ancestral COs (predicted through linkage disequilibrium) and the COs derived from the segregating population. We delineated 73 small intervals (,26 kb) on chromosome 3B that contained 252 COs. We observed a significant association of COs with genic features (73 and 54% in recombinant and nonrecombinant intervals, respectively) and with those expressed during meiosis (67% in recombinant intervals and 48% in nonrecombinant intervals). Moreover, while the recombinant intervals contained similar amounts of retrotransposons and DNA transposons (42 and 53%), nonrecombinant intervals had a higher level of retrotransposons (63%) and lower levels of DNA transposons (28%). Consistent with this, we observed a higher frequency of a DNA motif specific to the TIR-Mariner DNA transposon in recombinant intervals. KEYWORDS recombination; meiosis; bread wheat; linkage disequilibrium; transposon; hotspot; sequence motif M EIOTIC recombination is a process that allows reshuffling of diversity by the reciprocal exchange of DNA called a crossover (CO). This phenomenon is conserved in most eukaryotes (for a review see Mercier et al. 2015) and follows the formation of a double-strand break (DSB) of DNA generated by the topoisomerase SPO11 complex. However, the number of DSBs is at least 10-to 50-fold greater than the number of COs which rarely exceeds three per bivalent chromosome per meiosis (Mercier et al. 2015). This paucity of COs per meiosis with regards to the number of DSBs suggests the existence of a tight control and regulation of recombination in plants that promote DSB repair in a manner that does not lead to COs. For example, the study of the two helicases AtFANCM and RECQ4 (AtRECQ4A and AtRECQ4B) (Crismani et al. 2012;Knoll et al. 2012;Girard et al. 2014;Séguéla-Arnaud et al. 2015) revealed three-and sixfold CO frequency increases in the Atfancm single mutant and the Atrecq4a/Atrecq4b double mutant, respectively, compared to the wild type. These increases result from additional COs from the class-II pathway which suggests that FANCM and RECQ4 prevent CO formation and direct recombination intermediates towar...

show abstract

“…Mutation of UVI4 affects the somatic cell cycle but not meiosis [27]. These two genes originated from a whole-genome duplication that is shared by all Brassicaceae [28]. Accordingly, two genes representative of this family are typically found in Brassicaceae species and sequence similarity analysis clearly distinguishes the OSD1 proteins from the UVI4 proteins ( Figure 2).…”

Section: Identification Of Ososd1mentioning

confidence: 99%

Turning rice meiosis into mitosis

Mieulet¹,

Jolivet²,

Rivard³

et al. 2016

Cell Res

132

View full text Add to dashboard Cite

Introduction of clonal reproduction through seeds (apomixis) in crops has the potential to revolutionize agriculture by allowing self-propagation of any elite variety, in particular F1 hybrids. In the sexual model plant Arabidopsis thaliana synthetic clonal reproduction through seeds can be artificially implemented by (i) combining three mutations to turn meiosis into mitosis (MiMe) and (ii) crossing the obtained clonal gametes with a line expressing modified CENH3 and whose genome is eliminated in the zygote. Here we show that additional combinations of mutations can turn Arabidopsis meiosis into mitosis and that a combination of three mutations in rice (Oryza sativa) efficiently turns meiosis into mitosis, leading to the production of male and female clonal diploid gametes in this major crop. Successful implementation of the MiMe technology in the phylogenetically distant eudicot Arabidopsis and monocot rice opens doors for its application to any flowering plant and paves the way for introducing apomixis in crop species.

show abstract

Meiotic Gene Evolution: Can You Teach a New Dog New Tricks?

Cited by 54 publications

References 25 publications

Gene Duplicability of Core Genes Is Highly Consistent across All Angiosperms

Gene Duplicability of Core Genes Is Highly Consistent across All Angiosperms

High-Resolution Mapping of Crossover Events in the Hexaploid Wheat Genome Suggests a Universal Recombination Mechanism

Turning rice meiosis into mitosis

Contact Info

Product

Resources

About