Meiotic study of Zea mays ssp. mays (2n = 40) x Tripsacum dactyloides (2 n = 72) hybrid and its progeny

Molina, Marı́a del Carmen; García, María Dina; Chorzempa, Silvia Elena

doi:10.2225/vol9-issue3-fulltext-25

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…In the P-genome (T-genome) from tripsazea, the percentage of chromosome participating in heterologous pairing was 31.11% (1.70%). These results, together with previous research (De Wet and Harlan 1974;Molina et al 2006;Iqbal et al 2018), revealed that a) T. dactyloides is more closely related to Z. mays than Z. perennis, b) three genomes sharing one nucleus led to a reduction in heterologous pairings in comparison with an intrageneric dihybrid, and c) intergenomic pairing seemed to be higher but still rare in the trihybrid in comparison with its intergeneric dihybrid parent.…”

Section: Discussion Previous Trihybrids Involving Maize Tripsacum Ansupporting

confidence: 78%

“…These authors and De developed the second type of Tripsacum bridge (2n = 3x = 10Zm + 36Td, MTT) from a cross of diploid Z. mays • tetraploid T. dactyloides. Kindiger et al (1996a) and Molina et al (2006) reported the development of the third type of Tripsacum bridge (2n = 4x = 20Zm + 36Td, MMTT) from a cross between tetraploid Z. mays and tetraploid T. dactyloides. Backcrossed derivatives of three types of Tripsacum bridges with maize are also Tripsacum bridges and the only pathway to improve maize by Tripsacum.…”

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confidence: 99%

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Tripsazea, a Novel Trihybrid of Zea mays, Tripsacum dactyloides, and Zea perennis

Cheng

et al. 2020

G3 Genes|Genomes|Genetics

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A trispecific hybrid, MTP (hereafter called tripsazea), was developed from intergeneric crosses involving tetraploid Zea mays (2n = 4x = 40, genome: MMMM), tetraploid Tripsacum dactyloides (2n = 4x = 72, TTTT), and tetraploid Z. perennis (2n = 4x = 40, PPPP). On crossing maize-Tripsacum (2n = 4x = 56, MMTT) with Z. perennis, 37 progenies with varying chromosome numbers (36-74) were obtained, and a special one (i.e., tripsazea) possessing 2n = 74 chromosomes was generated. Tripsazea is perennial and expresses phenotypic characteristics affected by its progenitor parent. Flow cytometry analysis of tripsazea and its parents showed that tripsazea underwent DNA sequence elimination during allohexaploidization. Of all the chromosomes in diakinesis I, 18.42% participated in heterogenetic pairing, including 16.43% between the M- and P-genomes, 1.59% between the M- and T-genomes, and 0.39% in T- and P-genome pairing. Tripsazea is male sterile and partly female fertile. In comparison with previously synthesized trihybrids containing maize, Tripsacum and teosinte, tripsazea has a higher chromosome number, higher seed setting rate, and vegetative propagation ability of stand and stem. However, few trihybrids possess these valuable traits at the same time. The potential of tripsazea is discussed with respect to the deployment of the genetic bridge for maize improvement and forage breeding.

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Section: Discussion Previous Trihybrids Involving Maize Tripsacum Ansupporting

confidence: 78%

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confidence: 99%

Tripsazea, a Novel Trihybrid of Zea mays, Tripsacum dactyloides, and Zea perennis

Cheng

et al. 2020

G3 Genes|Genomes|Genetics

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“…Pollen grains viability was estimated using Lugol's iodine staining technique (Molina et al, 2006). Pollen grains were stained a dark-blue being considered viable, while those non stained pollen grains were considered non viable.…”

Section: Determination Of Meiotic Chromosomal Anomalies and Pollen Grains Viabilitymentioning

confidence: 99%

Meiotic Chromosomal Anomalies Resulting From Fungal Infection of Maize Compared With Those Resulting From Gamma Irradiation

El-Diasty

Zaied

Kosba

et al. 2009

Journal of Agricultural Chemistry and Biotechnology

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Meiotic chromosomal anomalies resulting from fungal infection with Fusarium verticillioides on some maize hybrids in Egypt were studied compared with those resulting from gamma irradiation. Fungal infection and gamma irradiation treatments elicited similar types of chromosomal anomalies. The percentage of these chromosomal anomalies types differed between treatments. The results illustrated that the differences among all doses of radiation as well as between infection and uninfection treatments were more clearly in case of stickiness % and unorientation % at metaphasic stages, respectively. The results also indicated that the percentage of total abnormal meiotic cells (T. Ab.%) was increased as the doses of gammairradiation increased. Similarly, most of the mean values of T. Ab.% in case of infection were highest than their corresponding of uninfection. On the other hand, the results indicated diminishing the effect of infection with the exposure to radiation. In the same times, the results indicated that the infection treatments induced highly increases in multipolarity and bridges in case of non-irradiated plants compared with those resulting in case of irradiated plants. Generally, the results indicated that the fungal infection caused a significant increase in meiotic chromosomal anomalies percentage and significant decreased in pollen viability percentage. This effect of infection treatment were lowest than that the effect of irradiation treatment.

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“…Artificial crosses are possible between corn and T. dactyloides with more or less success but generally involve embryo rescue (Leblanc et al 2009;Belova et al 2010). Although F1 hybrids between maize and T. dactyloides showed an abnormal meiosis, pairing between chromosomes from both parental species indicated the possibility of genetic recombination between them (Molina et al 2006). Previous studies reported that maize chromosomes 2, 5, 8, and 9 have a potential for genetic exchange with T. dactyloides chromosomes (Galinat 1973).…”

Section: Introductionmentioning

confidence: 99%

Allopolyploidization facilitates gene flow and speciation among corn, Zea perennis and Tripsacum dactyloides

Iqbal

Cheng

et al. 2019

Planta

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Main conclusion Tripsacum dactyloides is closely related to Zea mays since Zea perennis and the MTP tri-species hybrid have four possible reproductive modes. Eastern gamagrass (Tripsacum dactyloides L.) and tetraploid perennial teosinte (Zea perennis) are well known to possess genes conferring resistance against biotic and abiotic stresses as well as adaptation to flood and aluminum toxic soils. However, plant breeders have been hampered to utilize these and other beneficial traits for maize improvement due to sterility in their hybrids. By crossing a tetraploid maize-inbred line × T. dactyloides, a female fertile hybrid was produced that was crossed with Z. perennis to yield a tri-genomic female fertile hybrid, which was backcrossed with diploid maize to produce BC 1 and BC 2. The tri-genomic hybrid provided a new way to transfer genetic material from both species into maize by utilizing conventional plant breeding methods. On the basis of cytogenetic observations using multi-color genomic in situ hybridization, the progenies were classified into four groups, in which chromosomes could be scaled both up and down with ease to produce material for varying breeding and genetic purposes via apomixis or sexual reproduction. In the present study, pathways were found to recover maize and to obtain specific translocations as well as a speedy recovery of the T. dactyloides-maize addition line in a second backcross generation. However, phenotypes of the recovered maize were in most cases far from maize as a result of genetic load from T. dactyloides and Z. perennis, and could not be directly used as a maize-inbred line but could serve as an intermediate material for maize improvement. A series of hybrids was produced (having varying chromosome number, constitution, and translocations) with agronomic traits from all three parental species. The present study provides an application of overcoming the initial interspecific barriers among these species. Moreover, T. dactyloides is closely related to Z. mays L. ssp. mays since Z. perennis and the MTP tri-species hybrid have four possible reproductive modes. Keywords Apomixes • Chromosome translocation • Eastern gamagrass • Germplasm expansion • Perennial tetraploid teosinte • Tri-genomic bridge plant Abbreviations mcGISH Multi-color Genomic in Situ-Hybridization MTP Maize, T. dactyloides, and Z. perennis hybrid Mz Maize, Zea mays L. ssp. mays Zp Zea perennis

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Meiotic study of Zea mays ssp. mays (2n = 40) x Tripsacum dactyloides (2 n = 72) hybrid and its progeny

Cited by 4 publications

References 15 publications

Tripsazea, a Novel Trihybrid of Zea mays, Tripsacum dactyloides, and Zea perennis

Tripsazea, a Novel Trihybrid of Zea mays, Tripsacum dactyloides, and Zea perennis

Meiotic Chromosomal Anomalies Resulting From Fungal Infection of Maize Compared With Those Resulting From Gamma Irradiation

Allopolyploidization facilitates gene flow and speciation among corn, Zea perennis and Tripsacum dactyloides

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