1998
DOI: 10.1210/endo.139.1.5699
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Mel 1a Melatonin Receptor Expression Is Regulated by Protein Kinase C and an Additional Pathway Addressed by the Protein Kinase C Inhibitor Ro 31–8220 in Ovine Pars Tuberalis Cells*

Abstract: The expression of the melatonin receptor is positively regulated by cAMP and negatively regulated by melatonin in the ovine pars tuberalis (PT). Furthermore, when PT cells are dispersed in primary culture, both messenger RNA (mRNA) and protein levels spontaneously increase through a process that can be blocked by melatonin, but does not involve cAMP. This suggests that other second messengers may be regulated by melatonin, which, in turn, regulates melatonin receptor mRNA and protein levels. In this study usin… Show more

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Cited by 16 publications
(4 citation statements)
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“…A substantial body of evidence supports a primary role for cAMP and downstream signaling events in mediating the effects of melatonin in the ovine PT cells (14, 16, 29 -33). In particular, elevation of cAMP has been shown to contribute to the regulation of the Mel 1a melatonin receptor mRNA and the clock gene oPer1 mRNA levels in PT cells (15,34,35). These observations support a role of central importance for cAMP signal transduction in regulating the state of activation of PT cells.…”
Section: Discussionmentioning
confidence: 60%
“…A substantial body of evidence supports a primary role for cAMP and downstream signaling events in mediating the effects of melatonin in the ovine PT cells (14, 16, 29 -33). In particular, elevation of cAMP has been shown to contribute to the regulation of the Mel 1a melatonin receptor mRNA and the clock gene oPer1 mRNA levels in PT cells (15,34,35). These observations support a role of central importance for cAMP signal transduction in regulating the state of activation of PT cells.…”
Section: Discussionmentioning
confidence: 60%
“…In ovine pars tuberalis cells, it has been shown that melatonin does not act through PKC activation to reduce mt1 mRNA expression and furthermore it has been suggested that other as yet undefined pathways must play an important role in the regulation of mt1 receptor by melatonin (6). Using GT1-7 cells, we have previously demonstrated that melatonin (1 nM) down-regulates GnRH gene expression in a 24-h cyclical pattern (24).…”
Section: Melatonin-mediated Regulation Of Gnrh Gene Expression In Gt1mentioning
confidence: 96%
“…A number of studies have investigated the signal transduction mechanisms in tissues associated with the hypothalamicpituitary-gonadal axis. At the level of the pituitary, melatonin inhibits adenylate cyclase through both PTX (pertussis toxin)-sensitive and PTX-insensitive G-proteins in ovine pars tuberalis cells (5,6). Melatonin also inhibits gonadotropin-releasing hormone (GnRH)-induced increases in cAMP, diacylglycerol, [Ca 2ϩ ] i , and c-Fos in neonatal rat gonadotrophs (7,8).…”
mentioning
confidence: 99%
“…PMA has been reported to cause a reduction in the levels of mt1 mRNA and protein expression in ovine pars tuberalis cells, indicating an inhibitory role for PKC in regulating melatonin membrane receptor [Barret et al, 1998]. Fig.…”
Section: Correlation Between Melatonin Receptors Expression and Enhanmentioning
confidence: 99%