Melaleuca quinquenervia essential oil inhibits α-melanocyte-stimulating hormone-induced melanin production and oxidative stress in B16 melanoma cells

Chao, Wen-Wan; Su, Chia‐Chi; Peng, Hsien Yu; Chou, Su‐Tze

doi:10.1016/j.phymed.2017.08.024

Cited by 51 publications

(55 citation statements)

References 61 publications

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“…To understand the mechanism by which 1c inhibits enzymatic activity of tyrosinase, we conducted in silico molecular docking simulation using AutoDock 4.2 software (SantaFe, NM, USA). Kojic acid has been widely used as a selective competitive inhibitor in numerous studies [ 33 , 34 ]. The docking simulation was effective with significant scores.…”

Section: Resultsmentioning

confidence: 99%

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

et al. 2018

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In this study, we designed and synthesized eight thiophene chalcone derivatives (1a–h) as tyrosinase inhibitors and evaluated their mushroom tyrosinase inhibitory activities. Of these eight compounds, (E)-3-(2,4-dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one (1c) showed strong competitive inhibition activity against mushroom tyrosinase with IC50 values of 0.013 μM for tyrosine hydroxylase and 0.93 μM for dopa oxidase. In addition, we used enzyme kinetics study and docking program to further evaluate the inhibitory mechanism of 1c toward tyrosinase. As an underlying mechanism of 1c mediated anti-melanogenic effect, we investigated the inhibitory activity against melanin contents and cellular tyrosinase in B16F10 melanoma cells. As the results, the enzyme kinetics and docking results supports that 1c highly interacts with tyrosinase residues in the tyrosinase active site and it can directly inhibit tyrosinase as competitive inhibitor. In addition, 1c exhibited dose-dependent inhibitory effects in melanin contents and intracellular tyrosinase on α-MSH and IBMX-induced B16F10 cells. Overall, our results suggested that 1c might be considered potent tyrosinase inhibitor for use in the development of therapeutic agents for diseases associated with hyperpigment disorders.

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Section: Resultsmentioning

confidence: 99%

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

et al. 2018

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“…In addition, Melanogenesis is described to result in the generation of free radicals like hydrogen peroxide in the melanocytes which again result in oxidative stress. Therefore ROS plays a significant role in the regulation of melanin synthesis and so ROS scavengers help in reduce the UV-induced melanogenesis [5]. Hyperpigmentation can be reduced by free radical scavengers and tyrosinase inhibitors that suppress melanogenesis in the skin [6].…”

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confidence: 99%

Comparative study on skin protection activity of polyphenol-rich extract and polysaccharide-rich extract from Sargassum vachellianum

Jesumani

Pei

et al. 2020

PLoS ONE

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Seaweed polyphenols and polysaccharide plays a broad range of biological activity. The objective of the present study was to study and compare the skin protection activity of fucoidan rich polysaccharide extract (SPS) and polyphenol-rich extract (SPP) from the seaweed Sargassum vachellianum. The skin protection activity was analyzed based on their ability to scavenge free radicals such as hydrogen peroxide and hydroxyl radicals, UV absorption potential, tyrosinase inhibition, moisture preservation, and antibacterial activity. From the results, both SPP and SPS protects the skin from UV damage. SPP showed good free radical scavenging ability, antimicrobial activity against E.coli and S. aureus and effectively absorbed the UVB and UVA rays whereas SPS hardly absorbs the UVA and UVB rays and showed weak free radical scavenging ability and no antimicrobial activity. SPS showed considerable inhibition on tyrosinase (51.21%) and had better moisture absorption (52.1%) and retention (63.24%) abilities than SPP. The results specified that both SPS and SPP have balancing potential on skin protection and suitable combinations of both could act as an active ingredient in cosmetics.calculating skin-lightening activity. In addition, Melanogenesis is described to result in the generation of free radicals like hydrogen peroxide in the melanocytes which again result in oxidative stress. Therefore ROS plays a significant role in the regulation of melanin synthesis and so ROS scavengers help in reduce the UV-induced melanogenesis [5]. Hyperpigmentation can be reduced by free radical scavengers and tyrosinase inhibitors that suppress melanogenesis in the skin [6]. Moisture absorption and retention capacity of the skin is getting disturbed due to age factor and exposure to environmental stress. Once the moisture in the skin is reduced, then the skin will become dry with wrinkles which also results in premature aging. Retaining moisture in the skin is also another important function of the skincare products. Compound that is rich in antioxidant activity associated with anti-tyrosinase and moisture-preserving properties and also has the ability to block the UV rays plays an important role in skin whitening and sunscreen products.Many synthetic chemicals like arbutin, hydroquinone and kojic acid are being used as tyrosinase inhibitors in skin whitening product, but it was testified to side effect like dermatitis, genotoxicity and also induce cancer. Hence, the exploration of an effective skin care agent is still continuing in cosmetic research. In recent years, natural products have become more attractive in the cosmetic industry due to no side effects. The sea harbors a natural compound that is promising to act as biologically active metabolites. Marine algae are highly studied for their potent antioxidant capability [7,8,9,10]. The seaweed bioactive compound can play a vital role in skincare formulation because of its antioxidant properties. The active compound protects the skin in several ways, which includes scavenging the ROS, supp...

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“…Indeed, a previous report has shown that essential oil of Zingiber officinale rhizome contains numerous bioactive components, such as α-pinene, valencene, and zingiberene [ 21 ]. Moreover, anti-melanogenic effects of α-pinene and valencene have also been observed in B16F10 mouse melanoma cells [ 22 , 23 ]. In addition, melanogenesis-inhibitory effect of [ 6 ]-shogaol, the major shogaol in Zingiber officinale rhizomes, has been observed to be through acceleration of ERK1/2-mediated MITF degradation [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells

Jung

Lee

et al. 2018

IJMS

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Zerumbone (ZER), an active constituent of the Zingiberaceae family, has been shown to exhibit several biological activities, such as anti-inflammatory, anti-allergic, anti-microbial, and anti-cancer; however, it has not been studied for anti-melanogenic properties. In the present study, we demonstrate that ZER and Zingiber officinale (ZO) extract significantly attenuate melanin accumulation in α-melanocyte-stimulating hormone (α-MSH)-stimulated mouse melanogenic B16F10 cells. Further, to elucidate the molecular mechanism by which ZER suppresses melanin accumulation, we analyzed the expression of melanogenesis-associated transcription factor, microphthalmia-associated transcription factor (MITF), and its target genes, such as tyrosinase, tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (TYRP2), in B16F10 cells that are stimulated by α-MSH. Here, we found that ZER inhibits the MITF-mediated expression of melanogenic genes upon α-MSH stimulation. Additionally, cells treated with different concentrations of zerumbone and ZO showed increased extracellular signal-regulated kinases 1 and 2 (ERK1/2) phosphorylation, which are involved in the degradation mechanism of MITF. Pharmacological inhibition of ERK1/2 using U0126 sufficiently reversed the anti-melanogenic effect of ZER, suggesting that increased phosphorylation of ERK1/2 is required for its anti-melanogenic activity. Taken together, these results suggest that ZER and ZO extract can be used as active ingredients in skin-whitening cosmetics because of their anti-melanogenic effect.

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Melaleuca quinquenervia essential oil inhibits α-melanocyte-stimulating hormone-induced melanin production and oxidative stress in B16 melanoma cells

Cited by 51 publications

References 61 publications

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

A Potent Tyrosinase Inhibitor, (E)-3-(2,4-Dihydroxyphenyl)-1-(thiophen-2-yl)prop-2-en-1-one, with Anti-Melanogenesis Properties in α-MSH and IBMX-Induced B16F10 Melanoma Cells

Comparative study on skin protection activity of polyphenol-rich extract and polysaccharide-rich extract from Sargassum vachellianum

Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells

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