Melanocyte stem cells and hair graying

Zhang, Xiaojiao; Zhu, Jing; Zhang, Jiezhi; Zhao, Hengguang

doi:10.1111/jocd.15652

Cited by 5 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the reasonable explanations could be the fact that melatonin may be metabolized through indolic and kynurenic pathways targeting the appearance of AFMK, 6(OH)MEL or 5-MT in melanoma cells [51]. To date, it was wellreported that the lightening effects of melatonin on the skin of lower vertebrates and inhibition of pigmentation in some furry animals are well appreciated [4,52]. In our report, we comparatively assessed the effect of MEL, AFMK, 6(OH)MEL and 5-MT where we not only noticed significant attenuation of TYR activity and melanin content but also a reduction of cAMP and downstream MITF responses.…”

Section: Discussionmentioning

confidence: 99%

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Möller,

Linowiecka,

Gagat

et al. 2023

IJMS

View full text Add to dashboard Cite

Melatonin (N-acetyl-5-methoxytryptamine, MEL), its kynurenic (N1-acetyl-N2-formyl-5-methoxykynurenine, AFMK) and indolic derivatives (6-hydroxymelatonin, 6(OH)MEL and 5-methoxytryptamine, 5-MT) are endogenously produced in human epidermis. Melatonin, produced by the pineal gland, brain and peripheral organs, displays a diversity of physiological functions including anti-inflammatory, immunomodulatory, and anti-tumor capacities. Herein, we assessed their regulatory effect on melanogenesis using amelanotic (A375, Sk-Mel-28) and highly pigmented (MNT-1, melanotic) human melanoma cell lines. We discovered that subjected compounds decrease the downstream pathway of melanin synthesis by causing a significant drop of cyclic adenosine monophosphate (cAMP) level, the microphthalmia-associated transcription factor (MITF) and resultant collapse of tyrosinase (TYR) activity, and melanin content comparatively to N-phenylthiourea (PTU, a positive control). We observed a reduction in pigment in melanosomes visualized by the transmission electron microscopy. Finally, we assessed the role of G-protein-coupled seven-transmembrane-domain receptors. Obtained results revealed that nonselective MT1 and MT2 receptor antagonist (luzindole) or selective MT2 receptor antagonist (4-P-PDOT) did not affect dysregulation of the melanin pathway indicating a receptor-independent mechanism. Our findings, together with the current state of the art, provide a convenient experimental model to study the complex relationship between metabolites of melatonin and the control of pigmentation serving as a future and rationale strategy for targeted therapies of melanoma-affected patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Möller,

Linowiecka,

Gagat

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

Abnormalities of Pigmentation

Pyeritz

2025

Emery and Rimoin’s Principles and Practice of Medical Genetics and Genomics

View full text Add to dashboard Cite

Deconvolving organogenesis in space and time via spatial transcriptomics in thick tissues

Asami,

Yin,

Garza

et al. 2024

Preprint

View full text Add to dashboard Cite

Organ development is guided by a space-time landscape that constraints cell behavior. This landscape is challenging to characterize for the hair follicle — the most abundant mini organ — due to its complex microscopic structure and asynchronous development. We developed 3DEEP, a tissue clearing and spatial transcriptomic strategy for characterizing tissue blocks up to 400 μm in thickness. We captured 371 hair follicles at different stages of organogenesis in 1 cubic millimeter of skin of a 12-hour-old mouse with 6 million transcripts from 81 genes. From this single time point, we deconvoluted follicles by age based on whole-organ molecular pseudotimes to animate a stop-motion 3D atlas of follicle development along its trajectory. We defined molecular stages for hair follicle organogenesis and characterized the order of emergence for its structures, differential signaling dynamics at its top and bottom, morphogen shifts preceding and accompanying structural changes, and series of structural changes leading to the formation of its canal and opening. We further found that hair follicle stem cells and their niche are established and stratified early in organogenesis, before the formation of the hair bulb. Overall, this work demonstrates the power of increased depth of spatial transcriptomics to provide a four-dimensional analysis of organogenesis.

show abstract

Melanocyte stem cells and hair graying

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 5 publications

References 39 publications

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Abnormalities of Pigmentation

Deconvolving organogenesis in space and time via spatial transcriptomics in thick tissues

Contact Info

Product

Resources

About