Melanoma Metastasis

Fidler, Isaiah J.

doi:10.1177/107327489500200503

Cited by 22 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After transferring protein to a PVDF membrane, the membrane was blocked with 5% non-fat milk for 1h at room temperature and incubated with primary antibodies in blocking solution at 4°C overnight. The mem-year survival rate in the United States is 98% among the patients with localized disease and 17% in the metastatic group [13]. NPS-2143 (hydrochloride) is a calcification drug that acts as an antagonist at the Calcium-Sensing Receptor (CaSR), and consequently stimulates the release of parathyroid hormone [14].…”

Section: Western Blotmentioning

confidence: 99%

NPS - 2143 (hydrochloride) inhibits melanoma cancer cell proliferation and induces autophagy and apoptosis

2018

View full text Add to dashboard Cite

> Melanoma is a common and aggressive skin cancer caused by the oncogenic transformation of melanocytes. NPS-2143 (hydrochloride) is a calcification drug that acts as an antagonist of the calcium-sensing receptor (CaSR) and consequently stimulates the release of parathyroid hormone. In the present work, we treated cells from the human melanoma cell line M14 to investigate the effects of NPS-2143 on melanoma cells and elucidate their underlying mechanisms. We observed that NPS-2143 inhibits the survival and proliferation of M14 cells and suppresses the migration and proliferation of M14 cells by inducing apoptosis. The Bax/Bcl-2 ratio in M14 cells was enhanced by the NPS-2143 treatment, suggesting that the mitochondrial apoptotic pathway was activated. The expression and phosphorylation of proteins involved in the PI3K signaling pathway were altered by NPS-2143 treatment. Our data show that NPS-2143 impacts the viability and induces the apoptosis of melanoma M14 cells through its impact on the PI3K signaling pathway. It suggests that NPS-2143 could represent a promising candidate for melanoma treatment. <

show abstract

Section: Western Blotmentioning

confidence: 99%

NPS - 2143 (hydrochloride) inhibits melanoma cancer cell proliferation and induces autophagy and apoptosis

2018

View full text Add to dashboard Cite

show abstract

“…Importantly the results show TNFerade ± RT was able to suppress LN metastases both at the draining iliac LN and also at the distal axillary LNs. These distinct LNs are likely seeded by different routes, through lymphatic or hematogenous metastatic dissemination [35,55,56]. Consequently, if TNFerade is acting only on the local tumor site it seems to be impacting both routes of dissemination.…”

Section: Discussionmentioning

confidence: 99%

Local gene delivery of tumor necrosis factor alpha can impact primary tumor growth and metastases through a host-mediated response

MacGill

Davis

Macko

et al. 2007

Clin Exp Metastasis

View full text Add to dashboard Cite

TNFerade is a replication incompetent adenovector designed to express human TNFalpha under control of the Egr-1 radiation and chemotherapy enhanced promoter, and is currently in Phase II/III clinical testing. Data from Phase I clinical testing of TNFerade in a limited set of melanoma patients suggested the potential to impact distal metastases following intratumoral injections of TNFerade. These clinical observations and the multiple potential mechanisms of TNFerade led us to hypothesize local treatment with TNFerade + radiation may impact metastatic disease. We explored this hypothesis in preclinical models using the spontaneously metastatic, syngeneic B16F10 murine melanoma model. Established subcutaneous B16F10 tumors were treated with intratumoral injections of TNFerade and localized 2 Gy fractionated radiation therapy, modeling the clinical treatment regimen. Following 10-14 days of treatment, mice were evaluated for metastases development in the iliac and axillary lymph nodes. Comparisons of metastatic burden to control groups indicated TNFerade +/- radiation suppressed the formation of metastases in the lymph nodes. Additional experiments in TNF receptor knockout mice, where the only possible effects are on tumor cells containing the TNFalpha receptor, indicate TNFerade's local and distal activities are critically dependent on a host-mediated response. These data provide direct preclinical evidence local therapy of a solid tumor with TNFerade can also reduce metastatic disease, in addition to effects on the treated lesion. Furthermore, our finding of a host dependant response(s) for TNFerade at both the treated tumor and on lymph node metastases suggest the potential for broad activity independent of tumor histology.

show abstract

“…This promotes the generation of a melanocytic clone with greater proliferative capacity than its surrounding cells, and as carcinogenesis progresses, these malignant cells gain plasticity and a rather specialized capacity for tissue invasion; this, in turn, allows them to reach the blood and lymphatic vessels. As a result, melanoma cells adhere to tissues such as the brain, liver, or even bones, culminating in the formation of metastases 5. 6…”

Section: Introductionmentioning

confidence: 99%

Improvement of a Gas Intake Device and a Gas Mixing Study in a Large‐Scale Vessel

Chen

Fan

2014

Chem Eng & Technol

View full text Add to dashboard Cite

Gas mixing is a common process in chemical engineering. For actual industrial manufacturing of chemicals, most reactions commonly run in reactors or reaction vessels with large volumes (over 10 m3). In order to simulate a gas mixing process in a sealed reactor, and finally to improve the mixing effect between the gases, here the mixing process between methane and air in a reaction vessel with a volume of 10 m3 was studied via several approaches. Several specific evaluation indexes, including the mixing degree, the maximum concentration difference, and the mixing time, were obtained and recorded to evaluate the mixing effect on the gases. After a series of experiments targeting the determination of the size and pressure difference values of the gas intake device, the mixing effect between methane and air was finally optimized. The mixing degree was found to reach 0.97 within 1 h of monitoring, and the mixing time was decreased by 1700 % from the original situation.

show abstract

Melanoma Metastasis

Cited by 22 publications

References 18 publications

NPS - 2143 (hydrochloride) inhibits melanoma cancer cell proliferation and induces autophagy and apoptosis

NPS - 2143 (hydrochloride) inhibits melanoma cancer cell proliferation and induces autophagy and apoptosis

Local gene delivery of tumor necrosis factor alpha can impact primary tumor growth and metastases through a host-mediated response

Improvement of a Gas Intake Device and a Gas Mixing Study in a Large‐Scale Vessel

Contact Info

Product

Resources

About