Melanopsin in chicken melanocytes and retina

Lima, Leonardo Henrique Ribeiro Graciani de; Scarparo, Ana Cristina; Isoldi, Mauro César; Visconti, Maria Aparecida; Castrucci, Ana Maria de Lauro

doi:10.1080/09291010600870230

Cited by 21 publications

(23 citation statements)

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“…Propidium iodide, protease inhibitor, PLC inhibitor, and other biochemical reagents were from Sigma-Aldrich;-pluronic acid F-127 and Fluo-3 AM were from Invitrogen-Molecular Probes; and serum free supplement (B-27 Supplement 50X) was from Invitrogen-Gibco (Grand Island, NY). The primary antibody against the chicken Opn4x was raised in rabbit with the specific melanopsin x peptide 1 RQKRDLLPDSYSCSEE, 23 the kind gift of Martin Zatz and Arjun Natesan (NIH, Bethesda, MD) and of Ignacio Provencio and Ana M. Castrucci (University of Virginia Charlottesville, VA); the anti-chicken Opn4m was raised in rat and generated with the specific Opn4m peptide: CKHGNRELQKQYHR (BioSynthesis Inc., Lewisville, TX). This antiserum recognizes only the chicken Opn4m and was tested by immunochemistry with positive immunostaining in chicken RGCs of the developing and mature retinas and in primary RGC cultures.…”

Section: Methodsmentioning

confidence: 99%

“…[17][18][19][20] In the chicken, the expression of Opn4 genes has been reported in diverse retinal cell layers, including RGCs of developing and mature retinas of wild-type animals and the inner retina of blind birds. 6,[17][18][19][20][21][22][23] These two genes encode at least five isoforms, [17][18][19][20] two of which show blue light sensitivity. 18 Moreover, other putative nonopsin photopigments, or cryptochromes (Crys), have been reported to be expressed in the chicken retina.…”

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confidence: 99%

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Light Activation of the Phosphoinositide Cycle in Intrinsically Photosensitive Chicken Retinal Ganglion Cells

Contín

Verra

Salvador

et al. 2010

Invest. Ophthalmol. Vis. Sci.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Light Activation of the Phosphoinositide Cycle in Intrinsically Photosensitive Chicken Retinal Ganglion Cells

Contín

Verra

Salvador

et al. 2010

Invest. Ophthalmol. Vis. Sci.

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“…Evidence suggests that during the course of evolution, mammals lost some visual opsins and Opn4x as they entered the nocturnal niche. In the chicken retina, different laboratories have reported the expression of Opn4 genes in outer nuclear, inner nuclear, and ganglion cell layer (GCL) cells (12)(13)(14)(15)(16)(17)(18)(19)(20)(21). At the protein level, OPN4M was shown to be restricted to ipRGCs, whereas OPN4X was found in the GCL at embryo day 8 (E8) but mostly in PROX1 (+) horizontal cells (HCs) by E15 (21); the homeobox gene PROX1 is a universal HC marker that is expressed from E8 forward (22,23).…”

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confidence: 99%

Horizontal cells expressing melanopsin x are novel photoreceptors in the avian inner retina

Morera

Díaz

Guido

2016

Proc. Natl. Acad. Sci. U.S.A.

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In the vertebrate retina, three types of photoreceptors-visual photoreceptor cones and rods and the intrinsically photosensitive retinal ganglion cells (ipRGCs)-converged through evolution to detect light and regulate image-and nonimage-forming activities such as photic entrainment of circadian rhythms, pupillary light reflexes, etc. ipRGCs express the nonvisual photopigment melanopsin (OPN4), encoded by two genes: the Xenopus (Opn4x) and mammalian (Opn4m) orthologs. In the chicken retina, both OPN4 proteins are found in ipRGCs, and Opn4x is also present in retinal horizontal cells (HCs), which connect with visual photoreceptors. Here we investigate the intrinsic photosensitivity and functioning of HCs from primary cultures of embryonic retinas at day 15 by using calcium fluorescent fluo4 imaging, pharmacological inhibitory treatments, and Opn4x knockdown. Results show that HCs are avian photoreceptors with a retinal-based OPN4X photopigment conferring intrinsic photosensitivity. Light responses in HCs appear to be driven through an ancient type of phototransduction cascade similar to that in rhabdomeric photoreceptors involving a G-protein q, the activation of phospholipase C, calcium mobilization, and the release of the inhibitory neurotransmitter GABA. Based on their intrinsic photosensitivity, HCs may have a key dual function in the retina of vertebrates, potentially regulating nonvisual tasks together with their sister cells, ipRGCs, and with visual photoreceptors, modulating lateral interactions and retinal processing.retina | light responses | horizontal cell | phototransduction | melanopsin I n the vertebrate retina, photoreceptors can be classified according to their function as canonical or noncanonical. The first group comprises specialized ciliary retinal neurons, cones, and rods in the outer retina, which participate in the image-forming processes associated with day/night vision. The second group is composed of intrinsically photosensitive retinal ganglion cells (ipRGCs) in the inner retina, which preferentially participate in the processing of photic inputs related to nonimage-forming tasks (photic synchronization of circadian rhythms, pupillary light reflexes, inhibition of pineal melatonin, etc.) (1-7). The photopigment melanopsin (OPN4) (1) confers photosensitivity to ipRGCs as clearly shown through later experiments with OPN4 knockout mice or OPN4 heterologous expression in nonretinal cells for loss or gain of function, respectively (8-11). Through evolution, OPN4 appears to have been encoded by at least two genes in vertebrates: Opn4x and Opn4m, the Xenopus and mammalian ortholog genes, respectively (12). Interestingly, the first-appearing vertebrates, nonmammals including fish, amphibians, and birds, possess both Opn4 genes whereas mammals only have Opn4m. Evidence suggests that during the course of evolution, mammals lost some visual opsins and Opn4x as they entered the nocturnal niche. In the chicken retina, different laboratories have reported the expression of Opn4 genes in outer nuclear...

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“…Melanopsina é a opsina não visual mais importante, encontrada na retina de todos os vertebrados, de peixes a humanos (Provencio et al, 2000;Bellingham et al, 202;Provencio et al, 2002;Drivenes et al, 2003;Chaurasia et al, 2005;Lima et al, 2006), e em cefalocordados (Koyanagi et al, 2004), apresenta maior semelhança estrutural (Provencio et al, 2000) e funcional Kumbalasiri et al, 2006) com as opsinas de invertebrados. Na retina de mamíferos, esse fotopigmento é expresso em uma pequena população de células ganglionares, que formam com sua extensa árvore dendrítica, uma extensa rede de células intrinsecamente fotossensíveis, as ipRGCs .…”

Section: Em 1998 Provencio E Colaboradores Clonaram De Melanóforos Dunclassified

Expressão gênica temporal de Melanopsina (Opn4), Clock, Cry e Per e sua regulação por melatonina em células de Danio rerio

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Lauro Castrucci por sua orientação e confiança durante todo o processo, desde o estágio até a conclusão deste trabalho. Seu apoio foi fundamental! Ao meu marido Marcelo pelo amor e dedicação. AGRADECIMENTOSMeus sinceros agradecimentos à Profa. Dra. Ana Maria de Lauro Castrucci pela oportunidade, confiança, dedicação e paciência. Mil palavras seriam insuficientes para expressar toda minha gratidão e admiração por esta profissional exemplar.À Profa. Dra. Maria Aparecida Visconti por seu apoio e pela leitura crítica deste trabalho.Ao Prof. Dr. Luiz S. Menna-Barreto e Daniela Wey pela contribuição nas análises de ritmo. Ao Leonardo por sua ajuda nas análises estatísticas e seu companheirismo durante esses anos.Ao pessoal do laboratório, pela experiência e contribuição.À querida Gláucia que com sua competência e sabedoria me ensinou e ajudou em tudo que precisei, além de ser uma profissional excelente é minha grande amiga de momentos bons e não tão bons.À Fernanda por seu carinho e sincera amizade, em qualquer hora e para qualquer coisa pude contar com sua ajuda.

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Melanopsin in chicken melanocytes and retina

Cited by 21 publications

References 56 publications

Light Activation of the Phosphoinositide Cycle in Intrinsically Photosensitive Chicken Retinal Ganglion Cells

Light Activation of the Phosphoinositide Cycle in Intrinsically Photosensitive Chicken Retinal Ganglion Cells

Horizontal cells expressing melanopsin x are novel photoreceptors in the avian inner retina

Expressão gênica temporal de Melanopsina (Opn4), Clock, Cry e Per e sua regulação por melatonina em células de Danio rerio

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