Melatonin alleviates arsenic (As) toxicity in rice plants via modulating antioxidant defense system and secondary metabolites and reducing oxidative stress

Jan, Rahmatullah; Asif, Saleem; Asaf, Sajjad; Lubna,; Du, Xiao-Xuan; Park, Jae-Ryoung; Kim, Nari; Bhatta, Dibya; Lee, In‐Jung; Kim, Kyung Min

doi:10.1016/j.envpol.2022.120868

Cited by 40 publications

(12 citation statements)

References 74 publications

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“…EH143 regulated vegetative indices, including the deposition of pigments such as anthocyanin and its biosynthesis, under stresses (Figure 2C). Similar results have been reported by 69 who showed the role of the arsenicmelatonin interaction in inducing anthocyanin biosynthesis, resulting in increases in cyanidin and delphinidin. Plant root exudates can provide the necessary nutrients for microorganisms to survive and protect these microorganisms from unfavorable living conditions.…”

Section: Discussionsupporting

confidence: 90%

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Kwon,

Adhikari,

Imran

et al. 2024

Journal of Pineal Research

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Recently, microorganism and exogenous melatonin application has been recognized as an efficient biological tool for enhancing salt tolerance and heavy metal detoxification in agriculture crops. Thus, the goal of this study was to isolate and evaluate a novel melatonin‐producing plant growth promoting bacterium. With high‐throughput whole genome sequencing, phytohormone measurements, expression profiling, and biochemical analysis, we can identify a novel PGPB that produces melatonin and unravel how it promotes soybean growth and development and protects against salt and Cd stress. We identify the melatonin synthesis pathway (tryptophan→tryptamine→serotonin melatonin) of the halotolerant (NaCl > 800 mM) and heavy metal‐resistant (Cd >3 mM) rhizobacterium Bacillus safensis EH143 and use it to treat soybean plants subjected to Cd and NaCl stresses. Results show that EH143 will highly bioaccumulate heavy metals and significantly improve P and Ca2+ uptake and the K+/Na+ (93%↑under salt stress) ratio while reducing Cd uptake (49% under Cd stress) in shoots. This activity was supported by the expression of the ion regulator HKT1, MYPB67, and the calcium sensors CDPK5 and CaMK1 which ultimately led to increased plant growth. EH143 significantly decreased ABA content in shoots by 13%, 20%, and 34% and increased SA biosynthesis in shoots by 14.8%, 31%, and 48.2% in control, salt, and Cd‐treated plants, upregulating CYP707A1 and CYP707A2 and PAL1 and ICS, respectively. The melatonin content significantly decreased along with a reduced expression of ASMT3 following treatment with EH143; moreover, reduced expression of peroxidase (POD) and superoxide dismutase (SOD) by 134.5% and 39% under salt+Cd stress, respectively and increased level of total amino acids were observed. Whole‐genome sequencing and annotation of EH143 revealed the presence of the melatonin precursor tryptophan synthase (trpA, trpB, trpS), metal and other ion regulators (Cd: cadA, potassium: KtrA and KtrB, phosphate: glpT, calcium: yloB, the sodium/glucose cotransporter: sgIT, and the magnesium transporter: mgtE), and enzyme activators (including the siderophore transport proteins yfiZ and yfhA, the SOD sodA, the catalase katA1, and the glutathione regulator KefG) that may be involved in programming the plant metabolic system. As a consequence, EH143 treatment significantly reduced the contents of lipid peroxidation (O2‐, MDA, and H2O2) up to 69%, 46%, and 29% in plants under salt+Cd stress, respectively. These findings suggest that EH143 could be a potent biofertilizer to alleviate NaCl and Cd toxicity in crops and serve as an alternative substitute for exogenous melatonin application.

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Section: Discussionsupporting

confidence: 90%

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Kwon,

Adhikari,

Imran

et al. 2024

Journal of Pineal Research

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“…As a multifunctional molecule, melatonin plays an essential role in regulating physiological and biochemical processes such as root development, seed germination, photosynthesis, stomatal movement, plant flowering, and post-ripening [ 21 , 22 , 23 ]. More importantly, melatonin is involved in the response to multiple abiotic stresses, including drought, salt, low/high temperatures, heavy metals, and nutrient deficits [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. There have been some reports on the mitigating effects of melatonin on Cr toxicity.…”

Section: Introductionmentioning

confidence: 99%

Melatonin Alleviates Chromium Toxicity in Maize by Modulation of Cell Wall Polysaccharides Biosynthesis, Glutathione Metabolism, and Antioxidant Capacity

Yang

Ren

Lin

et al. 2023

IJMS

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Melatonin, a pleiotropic regulatory molecule, is involved in the defense against heavy metal stress. Here, we used a combined transcriptomic and physiological approach to investigate the underlying mechanism of melatonin in mitigating chromium (Cr) toxicity in Zea mays L. Maize plants were treated with either melatonin (10, 25, 50 and 100 μM) or water and exposed to 100 μM K2Cr2O7 for seven days. We showed that melatonin treatment significantly decreased the Cr content in leaves. However, the Cr content in the roots was not affected by melatonin. Analyses of RNA sequencing, enzyme activities, and metabolite contents showed that melatonin affected cell wall polysaccharide biosynthesis, glutathione (GSH) metabolism, and redox homeostasis. During Cr stress, melatonin treatment increased cell wall polysaccharide contents, thereby retaining more Cr in the cell wall. Meanwhile, melatonin improved the GSH and phytochelatin contents to chelate Cr, and the chelated complexes were then transported to the vacuoles for sequestration. Furthermore, melatonin mitigated Cr-induced oxidative stress by enhancing the capacity of enzymatic and non-enzymatic antioxidants. Moreover, melatonin biosynthesis-defective mutants exhibited decreased Cr stress resistance, which was related to lower pectin, hemicellulose 1, and hemicellulose 2 than wild-type plants. These results suggest that melatonin alleviates Cr toxicity in maize by promoting Cr sequestration, re-establishing redox homeostasis, and inhibiting Cr transport from the root to the shoot.

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“…Naringenin, cyanidin, delphinidin, and total flavonoids were extracted using a solvent mixture comprising 70 mL of methanol, water, formic acid, and trifluoroacetic acid in the ratio of 70:27:2:1 (v/v), following the procedure outlined by Jan et al (2023a). Each sample, with an approximate volume of 2 ml, was combined with 98 ml of 50% acetonitrile (CAN) containing 0.1% formic acid.…”

Section: Isolation Quantification Of Anthocyanin Total Flavonoids And...mentioning

confidence: 99%

“…NBP2-62160) by Novus, USA. The method was followed as described by Jan et al (2023a). Each of the experiment was repeated three times.…”

Section: Isolation Quantification Of Anthocyanin Total Flavonoids And...mentioning

confidence: 99%

Unveiling the protective role of anthocyanin in rice: insights into drought-induced oxidative stress and metabolic regulation

Jan,

Asif,

Asaf

et al. 2024

Front. Plant Sci.

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This study investigates the impact of anthocyanin treatment on rice plants under drought stress, focusing on phenotypic, molecular, and biochemical responses. Anthocyanin were treated to one month old plants one week before the droughtexposure. Drought stress was imposed by using 10% polyethylene glycol (PEG 6000). Anthocyanin-treated plants exhibited significant enhancements in various traits, including growth parameters and reproductive characteristics, under normal conditions. When subjected to drought stress, these plants displayed resilience, maintaining or improving essential morphological and physiological features compared to non-treated counterparts. Notably, anthocyanin application mitigated drought-induced oxidative stress, as evidenced by reduced levels of reactive oxygen species (ROS) and lipid membrane peroxidation. The study also elucidates the regulatory role of anthocyanins in the expression of flavonoid biosynthetic genes, leading to increased levels of key secondary metabolites. Furthermore, anthocyanin treatment influenced the levels of stress-related signaling molecules, including melatonin, proline, abscisic acid (ABA), and salicylic acid (SA), contributing to enhanced stress tolerance. The enzymatic activity of antioxidants and the expression of drought-responsive genes were modulated by anthocyanins, emphasizing their role in antioxidant defense and stress response. Additionally, anthocyanin treatment positively influenced macronutrient concentrations, particularly calcium ion (Ca+), potassium ion (K+), and sodium ion (Na+), essential for cell wall and membrane stability. The findings collectively highlight the multifaceted protective effects of anthocyanins, positioning them as potential key players in conferring resilience to drought stress in rice plants. The study provides valuable insights into the molecular and physiological mechanisms underlying anthocyanin-mediated enhancement of drought stress tolerance, suggesting promising applications in agricultural practices for sustainable crop production.

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Melatonin alleviates arsenic (As) toxicity in rice plants via modulating antioxidant defense system and secondary metabolites and reducing oxidative stress

Cited by 40 publications

References 74 publications

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Melatonin Alleviates Chromium Toxicity in Maize by Modulation of Cell Wall Polysaccharides Biosynthesis, Glutathione Metabolism, and Antioxidant Capacity

Unveiling the protective role of anthocyanin in rice: insights into drought-induced oxidative stress and metabolic regulation

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