Melatonin Alleviates Copper Toxicity via Improving Copper Sequestration and ROS Scavenging in Cucumber

Cao, Yunyun; Qi, Chuandong; Li, Shuangtao; Wang, Zhirong; Wang, Xiaoyun; Wang, Jinfang; Ren, Shuxin; Li, Xingsheng; Zhang, Na; Guo, Yang‐Dong

doi:10.1093/pcp/pcy226

Cited by 119 publications

(61 citation statements)

References 79 publications

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“…In cadmium-exposed oat roots, GSH was found to be associated with cadmium 66 . In cucumber, GSH chelated excess Cu 2+ and formed heavy metal complexes that were transported to the vacuole 24 . In our study, we found that with and without melatonin pretreatment increased the level of GSH in copper-stressed melon roots.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic and metabolomic analyses reveal that melatonin promotes melon root development under copper stress by inhibiting jasmonic acid biosynthesis

Zheng

et al. 2020

Hortic Res

100

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Melatonin has been shown to alleviate the effects of abiotic stress and to regulate plant development. Copper, a common heavy metal and soil pollutant, can suppress plant growth and development. In this work, we explored the protective effects of exogenous melatonin on lateral root formation in response to copper stress using melon seeds subjected to three germination treatments: CK1 (control), CK2 (300 μmol/L CuSO 4), and MT3 (300 μmol/L melatonin + 300 μmol/L CuSO 4). Melatonin pretreatment increased the antioxidant enzyme activities and root vigor, and decreased the proline and malondialdehyde (MDA) contents in the roots of copper-stressed melon seedlings. We then used transcriptomic and metabolomic analyses to explore the mechanisms by which exogenous melatonin protects against copper stress. There were 70 significant differentially expressed genes (DEGs) (28 upregulated, 42 downregulated) and 318 significantly differentially expressed metabolites (DEMs) (168 upregulated, 150 downregulated) between the MT3 and CK2 treatments. Melatonin pretreatment altered the expression of genes related to redox and cell wall formation processes. In addition, we found that members of the AP2/ERF, BBR/BPC, GRAS, and HD-ZIP transcription factor families may have vital roles in lateral root development. Melatonin also increased the level of Glutathione (GSH), which chelates excess Cu 2+. The combined transcriptomic and metabolomic analysis revealed DEGs and DEMs involved in jasmonic acid (JA) biosynthesis, including four lipoxygenase-related genes and two metabolites (linoleic acid and lecithin) related to melatonin's alleviation effect on copper toxicity. This research elucidated the molecular mechanisms of melatonin's protective effects in copper-stressed melon.

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Section: Discussionmentioning

confidence: 99%

“…GSH can chelate Cu 2+ in cucumber under copper stress 24 . Our results showed that GSH exhibited different levels in different treatments.…”

Section: Melatonin Increased Glutathione Levels To Promote Copper Chementioning

confidence: 98%

Transcriptomic and metabolomic analyses reveal that melatonin promotes melon root development under copper stress by inhibiting jasmonic acid biosynthesis

Zheng

et al. 2020

Hortic Res

100

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“…Thus, the accepted view is that melatonin is a growth regulator in plants, with ability to control the root growth (Park & Back, 2012; Pelagio‐Flores, Muñoz‐Parra, Ortiz‐Castro, & López‐Bucio, 2012; Zhang, Zhang, Zhao, Sun, & Guo, 2013), leaf senescence (Byeon et al, 2012; Shi, Reiter, Tan, & Chan, 2015; Wang et al, 2013), and promotion of photosynthesis (Tan et al, 2019). In addition to serving as a biostimulator, melatonin defends against multiple abiotic stresses, such as cold (Bajwa, Shukla, Sherif, Murch, & Saxena, 2014; Shi & Chan, 2014), drought (Wang et al, 2013), and heavy metals (Cai et al, 2017; Cao et al, 2019). Melatonin enhances salt tolerance in a broad range of plant species, including Malus hupehensis (Li et al, 2012), Bermuda grass (Shi et al, 2015), sunflower (Arora & Bhatla, 2017), soybean (Wei et al, 2015), cucumber (Zhang et al, 2014), Arabidopsis (Chen et al, 2017), maize (Jiang et al, 2016), wheat (Ke et al, 2018), and rice (Li, Yu, Cui, & Yin, 2017; Liang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K⁺ transporters and K⁺ homeostasis

Liu

Shabala

Zhang

et al. 2020

Plant Cell & Environment

130

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This study aimed to reveal the mechanistic basis of the melatonin-mediated amelioration of salinity stress in plants. Electrophysiological experiments revealed that melatonin decreased salt-induced K + efflux (a critical determinant of plant salt tolerance) in a dose-and time-dependent manner and reduced sensitivity of the plasma membrane K +-permeable channels to hydroxyl radicals. These beneficial effects of melatonin were abolished by NADPH oxidase blocker DPI. Transcriptome analyses revealed that melatonin induced 585 (448 up-and 137 down-regulated) and 59 (54 up-and 5 down-regulated) differentially expressed genes (DEGs) in the root tip and mature zone, respectively. The most noticeable changes in the root tip were melatonininduced increase in the expression of several DEGs encoding respiratory burst NADPH oxidases (OsRBOHA and OsRBOHF), calcineurin B-like/calcineurin B-like-interacting protein kinase (OsCBL/OsCIPK), and calcium-dependent protein kinase (OsCDPK) under salt stress. Melatonin also enhanced the expression of potassium transporter genes (OsAKT1, OsHAK1, and OsHAK5). Taken together, these results indicate that melatonin improves salt tolerance in rice by enabling K + retention in roots, and that the latter process is conferred by melatonin scavenging of hydroxyl radicals and a concurrent OsRBOHF-dependent ROS signalling required to activate stress-responsive genes and increase the expression of K + uptake transporters in the root tip.

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“…Enhanced photo-consumption of melatonin by free radicals contributes to decrease the indoleamine on exposure to daylight in Symbiodinium, a dinoflagellate, but may have varied before rising irreversibly some 2.4 billion years ago during the Great Oxidation Event (GOE), by enhanced free radical production in relation with daily light/dark changes [85]. Melatonin also enhances antioxidant enzyme activities [154] and regenerates endogenous antioxidants like glutathione [155]. Relations of melatonin with oxygen may be traced back to emergence of the latter gas in the Earth's atmosphere [83].…”

Section: The Redox System An Axismentioning

confidence: 99%

Melatonin Relations with Energy Metabolism as Possibly Involved in Fatal Mountain Road Traffic Accidents

Behn

Gregorio

2020

IJMS

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Previous results evidenced acute exposure to high altitude (HA) weakening the relation between daily melatonin cycle and the respiratory quotient. This review deals with the threat extreme environments pose on body time order, particularly concerning energy metabolism. Working at HA, at poles, or in space challenge our ancestral inborn body timing system. This conflict may also mark many aspects of our current lifestyle, involving shift work, rapid time zone crossing, and even prolonged office work in closed buildings. Misalignments between external and internal rhythms, in the short term, traduce into risk of mental and physical performance shortfalls, mood changes, quarrels, drug and alcohol abuse, failure to accomplish with the mission and, finally, high rates of fatal accidents. Relations of melatonin with energy metabolism being altered under a condition of hypoxia focused our attention on interactions of the indoleamine with redox state, as well as, with autonomic regulations. Individual tolerance/susceptibility to such interactions may hint at adequately dealing with body timing disorders under extreme conditions.

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Melatonin Alleviates Copper Toxicity via Improving Copper Sequestration and ROS Scavenging in Cucumber

Cited by 119 publications

References 79 publications

Transcriptomic and metabolomic analyses reveal that melatonin promotes melon root development under copper stress by inhibiting jasmonic acid biosynthesis

Transcriptomic and metabolomic analyses reveal that melatonin promotes melon root development under copper stress by inhibiting jasmonic acid biosynthesis

Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K⁺ transporters and K⁺ homeostasis

Melatonin Relations with Energy Metabolism as Possibly Involved in Fatal Mountain Road Traffic Accidents

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Melatonin Alleviates Copper Toxicity via Improving Copper Sequestration and ROS Scavenging in Cucumber

Cited by 119 publications

References 79 publications

Transcriptomic and metabolomic analyses reveal that melatonin promotes melon root development under copper stress by inhibiting jasmonic acid biosynthesis

Transcriptomic and metabolomic analyses reveal that melatonin promotes melon root development under copper stress by inhibiting jasmonic acid biosynthesis

Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K+ transporters and K+ homeostasis

Melatonin Relations with Energy Metabolism as Possibly Involved in Fatal Mountain Road Traffic Accidents

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Melatonin improves rice salinity stress tolerance by NADPH oxidase‐dependent control of the plasma membrane K⁺ transporters and K⁺ homeostasis