Melatonin alters the secondary metabolite profile of grape berry skin by promoting VvMYB14-mediated ethylene biosynthesis

Abstract

The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely unclear. Here, widely targeted metabolomics analysis revealed a total of 464 metabolites present in berry skin. Among them, 27 significantly differentially accumulated metabolites (DAMs) were produced in response to melatonin treatment in the presence or absence of 1-MCP. Most of the DAMs were secondary metabolites, including flavonoids, phenolic acids, stilbenes, and flavonols. Additionally, the accumulation of 25 DAMs was regulated by melatonin via ethylene. RNA-seq analysis indicated that melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ethylene. Gene-metabolite association analysis showed that melatonin regulated the expression of the VvSTS1, VvF3H, VvLAR2, and VvDFR genes, suggesting that these genes may play key roles in regulating secondary metabolites in the skin; additionally, VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary metabolites. Further experiments revealed that melatonin induced the expression of VvMYB14 and that VvMYB14 increased ethylene production by transcriptionally activating VvACS1, thereby affecting the accumulation of secondary metabolites. Collectively, melatonin promotes ethylene biosynthesis and alters secondary metabolite accumulation through the regulation of VvACS1 by VvMYB14.

McMYB4 improves temperature adaptation by regulating phenylpropanoid metabolism and hormone signaling in apple

“…Then, 200 ng of pGADT7-McMYB4 was added to an Eppendorf (EP) tube, 50 μl of Y1H gold ( pCAD-Bait-AbAi , pF5H-Bait-AbAi , pAUX-Bait-AbAi , pAUX Mutant-AbAi , pARF-Bait-AbAi , pARF Mutant-AbAi , pBRI-Bait-AbAi , pBRI Mutant-AbAi , pBIN-Bait-AbAi , and pBIN Mutant-AbAi ) was added, and Y1H gold ( p53-AbAi ) was used as a positive control. Cells at different concentrations were selected on SD/-Ura, SD/-Leu, and SD/-Leu/500 ng AbA culture plates, and positive colonies grew normally 53 .…”

“…The recombinant protein was purified using a One-Step MBP-Tagged Protein Mini-prep Pack (BioLab Co., Ltd., Beijing, China). EMSAs were conducted using the Light Shift ® Chemiluminescent EMSA Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol with 10 μg of purified McMYB4 protein and CHS / FLS / CAD / F5H and AUX / ARF / BRI / BIN probes with biotin-labeled oligonucleotides 53 .…”

Melatonin Relieves Ozone Stress in Grape Leaves by Inhibiting Ethylene Biosynthesis

“…The potted seedlings were cultivated in a greenhouse. When the new shoot leaves grew to 10-12 pieces, the plants with the same growth potential were selected and treated with water, 50 µM MT (Xu et al, 2019), 250 mg L −1 ethephon (Ma et al, 2021), or 2 µM aminoethoxyvinylglycine (AVG) (Xu et al, 2019) every 2 days at 6 p.m. (three times in total), and each treatment (1.5 L) was replicated in five plants. After that, the plants were exposed to 110 nL L −1 O 3 for 3 h at 800 µmol m −2 s −1 light intensity at 8 a.m. (Geng et al, 2016(Geng et al, , 2017.…”

Integrated Transcriptomics and Nontargeted Metabolomics Analysis Reveal Key Metabolic Pathways in Ganoderma lucidum in Response to Ethylene

“…Ethylene is thought to play an important role in both development and stress responses since it can regulate growth, tissue differentiation, maturation, and responses to numerous stresses. Additionally, ethylene is critical for secondary metabolite accumulation in plants [ 14 , 15 , 16 ]. Ethylene biosynthesis and signal transduction pathways in the model plant Arabidopsis thaliana are well-characterized [ 17 ].…”