Melatonin and Hyaluronic Acid Mixture as a Possible Therapeutic Agent in Dental Medicine

Abstract

The present work reports an efficient procedure for a simultaneous determination of melatonin (MEL) and hyaluronic acid (HA) mixture applying first derivative UV-Vis spectrophotometry. At selected wavelength (205 nm), the linearity range for each component of the mixture was established. The linear regression correlation coefficients for the direct UV-Vis spectra (RHA2 = 0.9627) and their first derivate (RHA2 = 0.9986) put in evidence a better fit for the derivative procedure. Through such simple analysis method, it was possible to emphasize that each component of MEL-HA mixture preserves its identity and characteristic properties. Consequently, the association of MEL and HA biomolecules could be used for obtaining a complex therapeutic agent with extensive applications in dental medicine.

Innovative Complex Formulation as Topical Treatment for Oral Health Improvment in Periodontal Disease

“…However, mechanical debridement has limitations, mostly regarding the inability to access deep pockets, surface irregularities, and furcation areas, therefore using adjunctive antimicrobial agents, to eradicate or reduce the numbers of pathogenic bacteria, is mandatory [6]. Besides the antibacterial effect, immunomodulator y and anti-inflammatory agents, addressing periodontal tissues, in conjunction with SRP, could significantly improve CAL and lead to PD reduction [15].…”

“…The natural hormone Melatonin (MEL) has important antioxidant, anti-inflammatory, anti-angiogenic role, also stimulating bone formation. The administration of MEL or synthetic analogs, in periodontal disease, either as systemic supplement [29] or topical cream [30], after SRP, improved periodontal parameters comparing to SRP alone [15].…”

“…The high molecular weight HA is anti-angiogenic, anti-inflammatory (inhibits endothelial cell growth, binds fibrinogen, reduces the recruitment of inflammator y cells, the levels of inflammatory cytokines and the migration of stem cells) and has a beneficiary role in tissue injury repair, wound healing and immunosuppression. The low molecular weight HA is pro-inflammatory and has pro-angiogenic activities [15,16,31]. However, both high and low molecular HA have bacteriostatic (especially against S. aureus and A. actinomycetemcomitans) [32] and fungistatic actions (against C. albicans) [33].…”

“…In our previous papers, we have proposed and analyzed, with promising results, a complex mixtures of MEL and HA [15,16] and also a complex formulation containing MEL, HA and T [17]. By including MZ, the active complex mixture will also address the anaerobic group of bacteria, harbored in the deep periodontal pockets, aiming to increase the efficacy of the topical treatment.…”

“…Beside the antibacterial effect, for reducing inflammatory parameters such as bleeding on probing (BOP) and PD, the use of hyaluronic acid (HA), melatonin (MEL) or the recently combined MEL and HA in a complex active compound, as adjunctive therapy after scaling and root planing (SRP) in periodontal compromise patients, was also considered [15][16][17].…”