Melatonin appears to protect against steroidogenic collapse in both mice fed with high‐fat diet and H ₂ O ₂ ‐treated TM3 cells

Abstract

High-fat diets (HFDs) are detrimental to steroidogenesis and male fertility. This study aimed to investigate the protective effects of melatonin (MT) treatment on testicular dysfunction in mice fed with HFD. C57BL/6J male mice were randomly divided into three groups: CTRL, HFD and HFD + MT. MT treatment mitigated the increase in body weight and adipose tissue in HFD-fed mice. Serum levels of sex hormones were improved upon MT supplementation, and the expression of the testosterone synthesis proteins, StAR and P450scc was rescued as well. MT treatment significantly up-regulated the expression of SIRT1, SOD2, and GPx4 and down-regulated the expression of GRP78 and CHOP, indicating an attenuation of oxidative stress (OS) and endoplasmic reticulum (ER) stress. In TM3 cells, MT treatment protected against H 2 O 2 -induced steroidogenic collapse by improving mitochondrial function and attenuating OS and ER stress. These results indicate that MT treatment can improve steroidogenesis in mice fed with HFD and may have therapeutic value in the treatment of obesity-associated hypogonadism. K E Y W O R D Shigh-fat-diet, Leydig cells, melatonin, oxidative stress, steroidogenesis

Protective effects of melatonin against oxidative stress induced by metabolic disorders in the male reproductive system: a systematic review and meta-analysis of rodent models

“…The rodents included in the studies comprised mice (n=3) ( 28 – 30 ) and rats (n=21) ( 17 , 18 , 31 – 49 ). Across the included studies, rodents were subjected to induction of diabetes (n=11) ( 18 , 28 , 31 , 34 , 35 , 38 – 41 , 46 , 48 ), obesity (n=8) ( 17 , 30 , 32 , 33 , 36 , 37 , 42 , 49 ), hyperthyroidism (n=3) ( 43 , 44 , 47 ) and hypothyroidism (n=1) ( 44 ), hyperlipidemia (n=1) ( 29 ), and food deprivation (n=1) ( 45 ). To induce diabetes, streptozotocin ( 18 , 28 , 34 , 35 , 38 – 40 , 46 , 48 ) and alloxan ( 31 , 41 ) were used.…”

Meta‐analysis and machine learning reveal the antiobesity effects of melatonin on obese rodents

“…To ensure the quality of the database, only articles from peer‐reviewed journals were further evaluated. After a rigorous assessment of the abstracts and full texts, a database was established for meta‐analysis and machine learning using 31 articles 7–11,20–45 . The recruited dataset comprised 286 effect sizes from the 31 studies ( k = 49 for body weight, k = 22 for liver weight, k = 17 for eWAT, k = 22 for GLU, k = 45 for TG, k = 36 for LDL, k = 39 for HDL, k = 36 for TC, and k = 20 for clock‐related genes).…”

Coenzyme Q10 and Melatonin for the Treatment of Male Infertility: A Narrative Review

“…Consequently, melatonin exerts anti-proliferative and anti-inflammatory effects on testicular macrophages [ 69 ] and mast cells [ 63 ], and its testicular concentrations demonstrated a negative correlation with the concentration of pro-apoptotic molecules [ 63 ] and the number of macrophages [ 70 ] in biopsies from infertile patients. In addition, melatonin functions as a free radical scavenger [ 71 ], preventing apoptosis and restoring testicular function [ 72 , 73 , 74 ]. Accordingly, melatonin treatment reduces the severity of induced testicular damage in animal models [ 75 , 76 , 77 , 78 , 79 , 80 , 81 ].…”