Melatonin biosynthesizing enzyme genes and clock genes in ovary and whole brain of zebrafish ( Danio rerio ): Differential expression and a possible interplay

Khan, Zeeshan Ahmad; Yumnamcha, Thangal; Rajiv, Chongtham; Devi, Haobijam Sanjita; Mondal, Gopinath; Devi, Sh. Dharmajyoti; Bharali, Rupjyoti; Chattoraj, Asamanja

doi:10.1016/j.ygcen.2016.05.014

Cited by 50 publications

(35 citation statements)

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“…Studies on tropical carp and zebrafish demonstrated the acceleration of oocyte maturation through melatonin and its receptor (Carnevali et al, ; Chattoraj et al, ; Chattoraj, Seth, & Maitra, ); our observed elevated ovarian melatonin and its receptor ( mtnr1aa and mtnr1ab ) in the zebrafish under DD conditions complements these previous findings. The presence of independent melatonin‐synthesizing machinery in various tissues, including the ovary of zebrafish and other fish (Khan et al, ; Rajiv et al, ), indicates that increased local, de novo synthesis of melatonin in the brain and ovary may enhance oocyte maturation − albeit, their mode of action may be different in each organ. One example of a possible conserved function comes from studies on mammals, which determined a role for melatonin in the maintenance of proper follicular progesterone production and during ovulation (El‐Raey et al, ; Maganhin et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies on zebrafish from our group demonstrated cross‐talk between the melatonin bio‐synthesizing machinery and clock‐associated genes in the whole brain and ovary (Khan et al, ), revealing an “orchestrate model” of synchronization (Khan, Sanjita Devi et al, ). The increase in ovarian melatonin receptors may be important for receiving melatonin signals from the brain, thereby coordinating and regulating ovarian melatonin bio‐synthesis (Khan et al, ) and/or inducing oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

“…Wild‐type, adult zebrafish ( Danio rerio ) were collected from the Indo‐Burma Biodiversity hotspot region of North East India, and acclimatized in 50‐L glass aquaria for 1–2 months at 28°C using a 100‐W immersion heater (RS Electrical, India), under LD photic condition (300 Lux with florescent tube; light was on at 05:00 hr and off at 17:00 hr with an automated timer) (Frontier Digital Timer, Taiwan) (Barney Reed, ), at the IBSD Zebrafish facility, Imphal in Manipur, India (Khan et al, ). The fish were fed three times a day: dry flakes (Taiyo Pet Products Pvt.…”

Section: Methodsmentioning

confidence: 99%

“…Tissue sampling was therefore scheduled for 17:00 hr after the completion of a 72‐hr period in all three experimental groups. During the collection of samples, irrespective of the photic condition, six ( n = 6) adult female zebrafish were anaesthetized in 0.1% tricaine (Sigma–Aldrich, St. Louis, MO) solution before they were euthanized by decapitation (Khan et al, ). Whole brain and ovary were removed by dissecting the skull and body cavity, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, this indoleamine acts on the gonad itself via the hypothalamo‐hypophyseal axis and/or receptor‐mediated or ‐independent direct actions (Chattoraj et al, ). Melatonin is de novo synthesized in the zebrafish ovary, and its interaction with the brain involves clock‐associated genes (Khan, Yumnamcha et al, ). Therefore, understanding of the overall interaction of the brain‐pituitary‐reproductive axis through photoperiod, melatonin, and Gnih is warranted.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis

et al. 2017

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Circadian cycles and photoperiod are known to influence reproductive physiology in several animals. Neuropeptides, such as gonadotropin-inhibitory hormone (GNIH) and gonadotropin-releasing hormone (GNRH), are influenced by melatonin in birds and mammals. The present study demonstrates the role of melatonin in oocyte maturation in the zebrafish (Danio rerio), via the brain-pituitary-reproductive axis, under different photic conditions. Melatonin was significantly higher both in the whole brain and ovary under continuous dark (DD) compared to continuous light (LL) conditions. Transcription of gnih in the brain was high in LL, but low in DD; similarly, melatonin exogenous treatment reduced gnih in cultured brain in a dose-dependent manner. Expression of gnrh3, however, was high in both continuous photic conditions (DD and LL), whereas fshb and lhb were high only during DD. kiss2, another neuropeptide, was high in LL, but kiss1 remain unchanged among the conditions. At the gonad level, expression of fshr, lhcgr, mtnr1aa, and mtnr1ab tracked with the expression of their respective ligand in DD and LL. The expression of mprb is high in DD ovary, although intra-ovarian growth factors (tgfb1a and bmp15) were low. The measured increased percentages of germinal vesicle breakdown, expression of Cyclin B1, and reduced Cdc2p34 phosphorylation are consistent with increased maturation in the dark. Our study thus links melatonin to the inhibition of gnih in the brain-pituitary-reproductive axis of zebrafish in response to photic conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis

et al. 2017

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Daily and Seasonal Expression Profile of Serum Melatonin and Its Biosynthesizing Enzyme Genes (tph1, aanat1, aanat2, andhiomt) in Pineal Organ and Retina: A Study under Natural Environmental Conditions in a Tropical Carp,Catla catla

et al. 2016

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The tropical carp Catla catla is gaining importance for the studies of the impact of environmental changes on aquatic animals due to its surface dwelling habitat. To date, no information is available on the transcriptional profile of melatonin biosynthesizing enzyme genes in any tropical carp under either natural or artificial photothermal conditions in pineal and retina. The present study is an attempt to demonstrate the temporal pattern of expression of melatonin biosynthesizing enzyme genes, tryptophan hydroxylase 1 (tph1), arylalkylamine N-acetyltransferase (aanat1 and aanat2), and hydroxyindole-O-methyltransferase (hiomt) collectively and simultaneously in pineal organ and retina in tropical fish, C. catla, on a daily and seasonal basis under natural environmental conditions along with the serum melatonin levels. Depending upon the changes of the natural photothermal conditions, in four phases of an annual cycle, the variation and/or shifting of the rhythm parameters of different melatonin biosynthesizing enzyme genes in these two organs are different. Moreover, relative expression of these genes varies based on tissue and season. The serum melatonin levels correspond to the expression pattern of pineal aanat2 and hiomt. This finding indicates a possible organization of melatonin biosynthesizing enzyme genes with reproductive phases differently in these two photoreceptive organs for maintaining its physiological functions.

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Rhythms in the endocrine system of fish: a review

2017

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The environment which living organisms inhabit is not constant and many factors, such as light, temperature, and food availability, display cyclic and predictable variations. To adapt to these cyclic changes, animals present biological rhythms in many of their physiological variables, timing their functions to occur when the possibility of success is greatest. Among these variables, many endocrine factors have been described as displaying rhythms in vertebrates. The aim of the present review is to provide a thorough review of the existing knowledge on the rhythms of the endocrine system of fish by examining the hormones that show rhythmicity, how environmental factors control these rhythms and the variation in the responses of the endocrine system depending on the time of the day. We mainly focused on the hypothalamic-pituitary axis, which can be considered as the master axis of the endocrine system of vertebrates and regulates a great variety of functions, including reproduction, growth, metabolism, energy homeostasis, stress response, and osmoregulation. In addition, the rhythms of other hormones, such as melatonin and the factors, produced in the gastrointestinal system of fish are reviewed.

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Melatonin biosynthesizing enzyme genes and clock genes in ovary and whole brain of zebrafish ( Danio rerio ): Differential expression and a possible interplay

Cited by 50 publications

References 64 publications

Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis

Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis

Daily and Seasonal Expression Profile of Serum Melatonin and Its Biosynthesizing Enzyme Genes (tph1, aanat1, aanat2, andhiomt) in Pineal Organ and Retina: A Study under Natural Environmental Conditions in a Tropical Carp,Catla catla

Rhythms in the endocrine system of fish: a review

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