Melatonin Can Not Significantly Increase Platelet Count

Abstract

Background: Burn injury defines as skin or tissue damage caused by heat, radioactivity, electricity, or chemicals. Burn injury have an impact on physiological homeostasis. Burn injury will cause an increase in free radicals, it can also cause changes in the platelet count. Melatonin has been proposed as burn supportive therapy because it acts as an antioxidant that can eliminate free radicals. Melatonin can increase the number of the platelets through the mechanism of increasing megakaryocyte fragmentation and modulating the cytokines involved in platelet production. Aim: Proving the effects of melatonin supplementation on the number of platelets in male Wistar rats with third degree of burn injury. Methods: This research was an experimental with randomized control group pre-post test design. Samples are 12 healthy male Wistar rats then randomly divide into two groups, control and experimental group. Each rat was induced 30% burn injury under anesthesia. Rats in control group were given placebo at 0, 8, and 16 hours after burn injury, while rats in experimental group were treated with melatonin intraperitoneal at 0, 8 and 16 hours after burn injury. Blood samples were collected from the retroorbital sinuses at 0, 3, and 24 hours. Data were analyzed statistically by Paired t-Test and Independent t-Test. Results: In control group, the number of platelets at 0-3 hours (p=0,024) and 0-24 hours (p=0,039) showed a significant decrease in platelet count. In experimental group, the number of platelets at 0-3 hours (p=0,047), 0-24 hours (0,015), 3-24 hours (p=0,04) showed a significant decrease in platelet count.Conclusions: Melatonin administration did not cause a significant increase of platelets number. Keywords: Burns, Platelet count, Melatonin

The Effect of Melatonin on Platelet Levels in Wistar Rat After Burns in Two Days

“…Then the platelets are mobilized for coagulation as part of the thrombotic process, resulting in a very high consumption of platelets 6 . The decrease in platelet levels in T2 is also in line with the results of previous studies where the administration of melatonin did not have an effect in the form of an increase in platelets at 24 hours after burn, but on the contrary, platelet levels continued to decrease for 24 hours after burns 13 .…”

“…Based on the explanation above, it is necessary to conduct further research on the effect of giving melatonin on platelet levels in Wistar rats by administering burn control within two days. The choice of a duration of two days was based on the results of a study on the effect of melatonin on platelets previously conducted by Tania Meysavira Fawzi, which obtained results of a less significant increase in platelet levels because the time was too short 13 . This is related to the formation of melatonin in the dark so that it will only cause significant changes in a period of more than 24 hours.…”