Melatonin-encapsuled silk fibroin electrospun nanofibers promote vascularized bone regeneration through regulation of osteogenesis-angiogenesis coupling

Deng, Lei; Hou, Mingzhuang; Lv, Nanning; Zhou, Quan; Hua, Xi; Hu, Xiayu; Ge, Xiaoyang; Zhu, Xuesong; Xu, Yong; Yang, Huilin; Chen, Xi; Liu, Hao; He, Fan

doi:10.1016/j.mtbio.2024.100985

Cited by 5 publications

(1 citation statement)

References 46 publications

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“…We also found that the controlled release of Mel from Zn–Ga:mHA/Mel nanoparticles improved new bone formation. Deng et al 103 developed Mel-encapsulated silk fibroin electrospun nanofibers and studied the role of Mel release on bone formation around critical-sized defects on the rat calvarium. Results revealed increased levels of osteogenesis-related markers (OCN) and angiogenesis-related markers (VEGF and CD31) in Mel-treated rats.…”

Section: Resultsmentioning

confidence: 99%

Melatonin-loaded mesoporous zinc- and gallium-doped hydroxyapatite nanoparticles to control infection and bone repair

Shokri,

Kharaziha,

Ahmadi Tafti

et al. 2024

Biomater. Sci.

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Section: Resultsmentioning

confidence: 99%

Melatonin-loaded mesoporous zinc- and gallium-doped hydroxyapatite nanoparticles to control infection and bone repair

Shokri,

Kharaziha,

Ahmadi Tafti

et al. 2024

Biomater. Sci.

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A facile nanopattern modification of silk fibroin electrospun scaffold and the corresponding impact on cell proliferation and osteogenesis

Liu,

Ouyang,

Yao

et al. 2024

Regenerative Biomaterials

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As a well-known natural protein biomaterial, silk fibroin (SF) has shown broad application prospects in typical biomedical fields. However, the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites and other bioactive peptide sequences, and there is still significant room for further improvement of their biological functions. Therefore, it is crucial to develop a facile and effective modification strategy for this widely researched biomaterial. In this study, the SF electrospun scaffold has been chosen as a typical SF biomaterial, and air plasma etching has been adopted as a facile nanopattern modification strategy to promote its biological functions. Results demonstrated that the plasma etching could feasibly and effectively create nano-island-like patterns on the complex surface of SF scaffolds, and the detailed nanopattern features could be easily regulated by adjusting the etching time. In addition, the mesenchymal stem cell responses have illustrated that the nanopattern modification could significantly regulate corresponding cell behaviors. Compared with the non-etched scaffold, the 10 min-etched scaffold (10E scaffold) significantly promoted stem cell proliferation and osteogenic differentiation. Moreover, 10E scaffold has also been confirmed to effectively accelerate vascularization and ectopic osteogenesis in vivo using a rat subcutaneous implantation model. However, the mentioned promoting effects would be weakened or even counteracted with the increase of etching time. In conclusion, this facile modification strategy demonstrated great application potential for promoting cell proliferation and differentiation. Thus, it provided useful guidance to develop excellent SF-based scaffolds suitable for bone and other tissue engineering.

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SUN1 inhibits osteogenesis and promotes adipogenesis of human adipose‐derived stem cells by regulating α‐tubulin and CD36 expression

Fan,

Zhu,

Liu

et al. 2024

J Cellular Molecular Medi

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Sad and UNC84 domain 1 (SUN1) is a kind of nuclear envelope protein with established involvement in cellular processes, including nuclear motility and meiosis. SUN1 plays an intriguing role in human adipose‐derived stem cells (hASCs) differentiation; however, this role remains largely undefined. This study was undertaken to investigate the role of SUN1 in hASCs differentiation, as well as its underlying mechanisms. Employing siRNAs, we selectively downregulated SUN1 and CD36 expression. Microtubules were depolymerized using nocodazole, and PPARγ was activated using rosiglitazone. Western blotting was performed to quantify SUN1, PPARγ, α‐tubulin, CD36, OPN, and adiponectin protein expression levels. Alkaline phosphatase and Oil red O staining were used to assess osteogenesis and adipogenesis, respectively. Downregulated SUN1 expression increased osteogenesis and decreased adipogenesis in hASCs, concomitant with upregulated α‐tubulin expression and downregulated CD36 expression, alongside reduced nuclear localization of PPARγ. Microtubule depolymerization increased CD36 expression. Rescue experiments indicated that microtubule depolymerization counteracted the downregulated SUN1‐induced phenotypic changes. This study demonstrates that SUN1 influences the differentiation of hASCs towards osteogenic and adipogenic lineages, indicating its essential role in cell fate.

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Melatonin-encapsuled silk fibroin electrospun nanofibers promote vascularized bone regeneration through regulation of osteogenesis-angiogenesis coupling

Cited by 5 publications

References 46 publications

Melatonin-loaded mesoporous zinc- and gallium-doped hydroxyapatite nanoparticles to control infection and bone repair

Melatonin-loaded mesoporous zinc- and gallium-doped hydroxyapatite nanoparticles to control infection and bone repair

A facile nanopattern modification of silk fibroin electrospun scaffold and the corresponding impact on cell proliferation and osteogenesis

SUN1 inhibits osteogenesis and promotes adipogenesis of human adipose‐derived stem cells by regulating α‐tubulin and CD36 expression

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