Melatonin enhances lipid production in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions via a multi-level mechanism

Zhao, Yongteng; Li, Dafei; Xu, Junwei; Zhao, Peng; Li, Tao; Ma, Huixian; Yu, Xuya

doi:10.1016/j.biortech.2018.03.014

Cited by 80 publications

(28 citation statements)

References 48 publications

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“…Likewise, elevated lipid accumulation was also observed on the 10th day of cultivation in nitrogen-starved cells. An increase in lipid content when subjected to nitrate limitation has been exhibited in microalgal strains, such as P. kessleri, Monoraphidium sp., Chlorella zofingiensis (Feng et al, 2012;Zhao et al, 2018;Shaikh et al, 2019). The morphology of the cells in nitrogen stress indicates changes in the form and size after the 4th day of starvation as confirmation by the NR microscopy by dwindling and acquiring lipids.…”

Section: Discussionmentioning

confidence: 69%

Nutrient Deprivation Mobilizes the Production of Unique Tocopherols as a Stress-Promoting Response in a New Indigenous Isolate Monoraphidium sp.

et al. 2020

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The economic feasibility of the next-generation algal biofuels rely largely on the simultaneous production of high-value-added bioproducts (HVABs) in these photosynthetic cell factories. In this study, a newly isolated indigenous microalga belonging to the selenastraceae family Monoraphidium sp. (designated as CABeR41) has been isolated and characterized for its relevant biofuel precursors along with other HVABs, i.e., tocopherols. Our preliminary data sets demonstrate accumulation of higher amounts of lipids (266.6 mg g −1 dcw) along with antioxidant molecules, such as αand δ-tocopherols (456 µg g −1 dcw and 974 µg g −1 dcw), simultaneously when subjected to nutrient deprivation. The total tocopherol content has been increased in nitrogen-deplete conditions to 1450.24 µg g −1 dcw from 638.2 µg g −1 dcw. In conclusion, bioprospecting of new isolates from natural habitats and fine-tuning of specific conditions without compromising growth has potential for developing into a sustainable industrial strain for concomitant production of biofuels and bioproducts.

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Section: Discussionmentioning

confidence: 69%

Nutrient Deprivation Mobilizes the Production of Unique Tocopherols as a Stress-Promoting Response in a New Indigenous Isolate Monoraphidium sp.

et al. 2020

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“…QLY with no change in biomass content, whereas plant growth regulator addition alone increased both the acyl CoA carboxylase and ME levels and decreased the PEPC level. The addition of plant growth regulator (melatonin) increased the lipid biosynthetic genes expression levels leading to the increase in lipid content [33]. It is thus likely that the improved lipid content in Chlorella sp.…”

Section: Effect Of Plant Hormones On Lipid Content and Yieldmentioning

confidence: 99%

Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids

Sivaramakrishnan

Incharoensakdi

2020

Biotechnol Biofuels

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Background: Omega-3 fatty acids have various health benefits in combating against neurological problems, cancers, cardiac problems and hypertriglyceridemia. The main dietary omega-3 fatty acids are obtained from marine fish. Due to the pollution of marine environment, recently microalgae are considered as the promising source for the omega-3 fatty acid production. However, the demand and high production cost associated with microalgal biomass make it necessary to implement novel strategies in improving the biomass and omega-3 fatty acids from microalgae. Results: Four plant hormones zeatin, indole acetic acid (IAA), gibberellic acid (GBA) and abscisic acid (ABA) were investigated for their effect on the production of biomass and lipid in isolated Chlorella sp. The cells showed an increase of the biomass and lipid content after treatments with the plant hormones where the highest stimulatory effect was observed in ABA-treated cells. On the other hand, IAA showed the highest stimulatory effect on the omega-3 fatty acids content, eicosapentaenoic acid (EPA) (23.25%) and docosahexaenoic acid (DHA) (26.06%). On the other hand, cells treated with ABA had highest lipid content suitable for the biodiesel applications. The determination of ROS markers, antioxidant enzymes, and fatty acid biosynthesis genes after plant hormones treatment helped elucidate the mechanism underlying the improvement in biomass, lipid content and omega-3 fatty acids. All four plant hormones upregulated the fatty acid biosynthesis genes, whereas IAA particularly increased omega-3-fatty acids as a result of the upregulation of omega-3 fatty acid desaturase. Conclusions: The contents of omega-3 fatty acids, the clinically important compounds, were considerably improved in IAA-treated cells. The highest lipid content obtained from ABA-treated biomass can be used for biodiesel application according to its biodiesel properties. The EPA and DHA enriched ethyl esters are an approved form of omega-3 fatty acids by US Food and Drug Administration (FDA) which can be utilized as the therapeutic treatment for the severe hypertriglyceridemia.

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“…Phytohormone could increase the antioxidant system in the microalgae, which keeps a redox balance state under stressful conditions. The combination of plant hormones with a two-stage culture system may be efficient in increasing the higher biomass and lipid production in microalgae [131,134]. For instance, the integration of a two-stage strategy (heterotrophic-photoautotrophic) with fulvic acid results in an increase of 54-65% in the lipid content of Monoraphidium sp.…”

Section: Inducer Additionmentioning

confidence: 99%

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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Microalgae have received widespread interest owing to their potential in biofuel production. However, economical microalgal biomass production is conditioned by enhancing the lipid accumulation without decreasing growth rate or by increasing both simultaneously. While extensive investigation has been performed on promoting the economic feasibility of microalgal-based biofuel production that aims to increase the productivity of microalgae species, only a handful of them deal with increasing lipid productivity (based on lipid contents and growth rate) in the feedstock production process. The purpose of this review is to provide an overview of the recent advances and novel approaches in promoting lipid productivity (depends on biomass and lipid contents) in feedstock production from strain selection to after-harvesting stages. The current study comprises two parts. In the first part, bilateral improving biomass/lipid production will be investigated in upstream measures, including strain selection, genetic engineering, and cultivation stages. In the second part, the enhancement of lipid productivity will be discussed in the downstream measure included in the harvesting and after-harvesting stages. An integrated approach involving the strategies for increasing lipid productivity in up- and down-stream measures can be a breakthrough approach that would promote the commercialization of market-driven microalgae-derived biofuel production.

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Melatonin enhances lipid production in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions via a multi-level mechanism

Cited by 80 publications

References 48 publications

Nutrient Deprivation Mobilizes the Production of Unique Tocopherols as a Stress-Promoting Response in a New Indigenous Isolate Monoraphidium sp.

Nutrient Deprivation Mobilizes the Production of Unique Tocopherols as a Stress-Promoting Response in a New Indigenous Isolate Monoraphidium sp.

Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

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