Melatonin finely tunes proliferation and senescence in hematopoietic stem cells

Cruciani, Sara; Garroni, Giuseppe; Pala, Renzo; Barcessat, Ana Rita Pinheiro; Facchin, Federica; Ventura, Carlo; Fozza, Claudio; Maioli, Margherita

doi:10.1016/j.ejcb.2022.151251

Cited by 7 publications

(3 citation statements)

References 74 publications

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“…The cell suspension obtained from each sample was mixed with 0.4% dye solution (Thermo Fisher Scientific, USA) in a proportion of 1:1, then counted using a LUNA-II TM Automated Cell Counter to obtain information about the cell viability and cell size distributions. The percentage of vital cells was calculated as the number of positive cells divided by the total number of counted cells (mean ± SD) [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of REAC Regenerative Endogenous Bioelectrical Cell Reprogramming on MCF7 Breast Cancer Cells

Fontani

Cruciani

Santaniello

et al. 2023

JPM

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Human breast adenocarcinoma is a form of cancer which has the tendency to metastasize to other tissues, including bones, lungs, brain, and liver. Several chemotherapeutic drugs are used to treat breast tumors. Their combination is used to simultaneously target different mechanisms involved in cell replication. Radio electric asymmetric conveyer (REAC) technology is an innovative technology, used both in vitro and in vivo, to induce cell reprogramming and counteract senescence processes. Within this context, we treated MCF-7 cells with a regenerative (RGN) REAC treatment for a period ranging between 3 and 7 days. We then analyzed cell viability by trypan blue assays and gene and protein expression by real time-qPCR and confocal microscope, respectively. We also detected the levels of the main proteins involved in tumor progression, DKK1 and SFRP1, by ELISA and cell senescence by β-galactosidase tests. Our results showed the ability of REAC RGN to counteract MCF-7 proliferation, probably inducing autophagy via the upregulation of Beclin-1 and LC3-I, and the modulation of specific tumorigenic biomarkers, such as DKK1 and SPFR1. Our results could suggest the application of the REAC RGN in future in vivo experiments, as an aid for the therapeutic strategies usually applied for breast cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Impact of REAC Regenerative Endogenous Bioelectrical Cell Reprogramming on MCF7 Breast Cancer Cells

Fontani

Cruciani

Santaniello

et al. 2023

JPM

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“…In addition, melatonin synthesis in bone marrow cells has been implicated in hematopoiesis and the regulation of circadian rhythms in bone marrow-derived cells. This area of research is expanding our understanding of the role of melatonin in bone health and its potential therapeutic applications in hematological disorders [ 31 ].…”

Section: Melatonin: a Regulator Of Circadian Rhythmsmentioning

confidence: 99%

Melatonin and Vascular Function

Mendes,

Queiroz,

Sena

2024

Antioxidants

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The indolamine hormone melatonin, also known as N-acetyl-5-methoxytrypamine, is frequently associated with circadian rhythm regulation. Light can suppress melatonin secretion, and photoperiod regulates melatonin levels by promoting its production and secretion at night in response to darkness. This hormone is becoming more and more understood for its functions as an immune-modulatory, anti-inflammatory, and antioxidant hormone. Melatonin may have a major effect on several diabetes-related disturbances, such as hormonal imbalances, oxidative stress, sleep disturbances, and mood disorders, according to recent research. This has raised interest in investigating the possible therapeutic advantages of melatonin in the treatment of diabetic complications. In addition, several studies have described that melatonin has been linked to the development of diabetes, cancer, Alzheimer’s disease, immune system disorders, and heart diseases. In this review, we will highlight some of the functions of melatonin regarding vascular biology.

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“…Oral administration of β-1,3-glucans to aged male mice modulated immunosenescence characterized by a significant increase in T helper cells, the delayed-type hypersensitivity response, and immunoglobulin production (Song et al 2020 ). The plant melatonin, i.e., phytomelatonin, is also emerging as a potential nutraceutical with pharmacological effects against cellular senescence and immunosenescence in experimental animals (Arnao and Hernández-Ruiz 2018 ; Atayik and Çakatay 2022a ; Cruciani et al 2022 ; Fernández-Ortiz et al 2022 ; Srinivasan et al 2005 ). The mechanisms governing the apparent cellular senescence and immunosenescence modulatory effects of phytochemicals are multifaceted but are linked to the longevity nutrient sensing pathways such as the Sirtuins and mTOR as well as transcription factors such as NRF-2 and NF-κB (Mannick et al 2014 ; Micó et al 2017 ; Robledinos-Antón et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Emerging cellular senescence-centric understanding of immunological aging and its potential modulation through dietary bioactive components

et al. 2022

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Immunological aging is strongly associated with the observable deleterious effects of human aging. Our understanding of the causes, effects, and therapeutics of aging immune cells has long been considered within the sole purview of immunosenescence. However, it is being progressively realized that immunosenescence may not be the only determinant of immunological aging. The cellular senescence-centric theory of aging proposes a more fundamental and specific role of immune cells in regulating senescent cell (SC) burden in aging tissues that has augmented the notion of senescence immunotherapy. Now, in addition, several emerging studies are suggesting that cellular senescence itself may be prevalent in aging immune cells, and that senescent immune cells exhibiting characteristic markers of cellular senescence, similar to non-leucocyte cells, could be among the key drivers of various facets of physiological aging. The present review integrates the current knowledge related to immunosenescence and cellular senescence in immune cells per se, and aims at providing a cohesive overview of these two phenomena and their significance in immunity and aging. We present evidence and rationalize that understanding the extent and impact of cellular senescence in immune cells vis-à-vis immunosenescence is necessary for truly comprehending the notion of an ‘aged immune cell’. In addition, we also discuss the emerging significance of dietary factors such as phytochemicals, probiotic bacteria, fatty acids, and micronutrients as possible modulators of immunosenescence and cellular senescence. Evidence and opportunities related to nutritional bioactive components and immunological aging have been deliberated to augment potential nutrition-oriented immunotherapy during aging.

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Melatonin finely tunes proliferation and senescence in hematopoietic stem cells

Cited by 7 publications

References 74 publications

Impact of REAC Regenerative Endogenous Bioelectrical Cell Reprogramming on MCF7 Breast Cancer Cells

Impact of REAC Regenerative Endogenous Bioelectrical Cell Reprogramming on MCF7 Breast Cancer Cells

Melatonin and Vascular Function

Emerging cellular senescence-centric understanding of immunological aging and its potential modulation through dietary bioactive components

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