“…In horses, in contrast to most domestic animals but similar to humans, mare or stallion subfertility is the main indication for the use of ART (Benammar et al, 2021; Hinrichs, 2018). Because of the poor development so far using in vitro fertilization, intra‐cytoplasmic sperm injection (ICSI) is the main technology used, which has not been associated with obvious developmental abnormalities in equidae (Clérico et al, 2020; Heras et al, 2017; Hinrichs, 2018; Valenzuela et al, 2018). Nevertheless, there is a general agreement regarding, first, the link between sperm DNA fragmentation and male infertility, and second, the possibility of inadvertently microinjecting a DNA‐damaged sperm during ICSI, resulting in worse embryo quality and lower reproductive results, as well as the future animal's health (Fernández‐Gonzalez et al, 2008).…”