Melatonin improves the therapeutic role of mesenchymal stem cells in diabetic rats

Abstract

received phosphate-buffered saline (PBS); melatonin group received melatonin (10 mg/kg b.wt./day for 2 months by oral gavage); diabetic untreated group; diabetic group treated with melatonin; diabetic group treated with MSCs (a single intravenous injection of 3 × 10 cell in PBS); and diabetic group co-treated with stem cells and melatonin. The results showed significant improvement in glucose, insulin, total antioxidant, and malondialdehyde level in diabetic rats treated with either MSCs alone or in combination with melatonin. The imumuno-histochemical analysis showed that MSCs and/or melatonin treatment reduced the rate of inflammation and apoptosis of the islet cells as well as increased the rate of pancreatic cell division. Such results were indicated by a significant improvement in the level of TNF-α, IL-10, PCNA, and caspase-3 to levels very close to the control. Co-treatment of MSCs and MT resulted in an improvement in the tissue of the pancreas and reduced number of damaged β-cells. It can be concluded that co-treatment of stem cells and melatonin has a significant role in restoring the structural and functional efficiency of β-cells in the pancreas more than stem cells alone. Such results may be due to the role of melatonin as an antioxidant in increasing the efficiency and vitality of stem cells.

Melatonin and Mesenchymal Stem Cells as a Key for Functional Integrity for Liver Cancer Treatment

“…In this regard, MSCs pulsed with tumor-derived microvesicles showed an enhanced antitumor activity in HCC [ 166 ]. Some previous report revealed the use of melatonin enhanced the potential therapeutic role MSCs in treatment various diseases such as acute kidney injury, metabolic syndromes including diabetes through various mechanisms that include through the activation of antioxidative pathways, inhibition of the inflammatory response and reduction of apoptosis and fibrosis [ 19 , 167 , 168 , 169 , 170 ]. More interestingly, few recent reports revealed the beneficial effects of the combination between melatonin and MSCs on targeting inflammation in HCC [ 171 , 172 , 173 , 174 , 175 ].…”

Melatonin plays critical role in mesenchymal stem cell-based regenerative medicine in vitro and in vivo

“…Thus, MSC transplantation is also taken into account for one of the effective treatments for diabetes. The cotreatment of MSCs and MT significantly improved the levels of glucose, insulin, total antioxidant, and malondialdehyde and reduced the number of damaged β-cells in diabetic rats compared with the MSC group alone [11]. Diabetic nephropathy (DN) is one of the microvascular complications of diabetes that may progress to a serious end-stage renal failure.…”

Treatment of refractory secondary hemophagocytic lymphohistiocytosis with umbilical cord mesenchymal stem cells

“…The MSC treatment was found to have a therapeutic effect on insulin resistance in this patient (who had type 2 diabetes), which is consistent with previous findings. 20,21 We conclude that treatment with MSCs may be beneficial for certain patients with refractory secondary HLH of unknown origin. We can confirm transient effects of MSC infusion in HLH and type 2 diabetes mellitus.…”

“…The MSC treatment was found to have a therapeutic effect on insulin resistance in this patient (who had type 2 diabetes), which is consistent with previous findings. 20,21…”