Melatonin mitigates thioacetamide-induced hepatic fibrosis via antioxidant activity and modulation of proinflammatory cytokines and fibrogenic genes

Lebda, Mohamed A.; Sadek, Kadry M.; Abouzed, Tarek K.; Tohamy, Hossam G.; El‐Sayed, Yasser S.

doi:10.1016/j.lfs.2017.11.036

Cited by 52 publications

(40 citation statements)

References 54 publications

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“…One-way analysis of variance (ANOVA) was used, followed by Posthoc least significant difference (LSD) test to evaluate the differences between the groups. For all comparison P<0.05 were considered as significant difference [28] .…”

Section: Resultsmentioning

confidence: 99%

Effect of Silymarin versus Silymarin and Green Coffee Extract on Thioacetamide Induced Liver Injury in Adult Male Albino Rats (Histological and Immunohistochemical Study)

Abdelaal

Mousa

Ahmed

2019

Egyptian Journal of Histology

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Background: Thioacetamide (TAA) is a potent hepatotoxic agent used to induce liver toxicity in experimental animal models. Protective role of silymarin in liver intoxication was proved. Coffee is the most popular beverage used in the world. Consumption of green coffee bean extract has many benefits on health in general, and liver in particular. Aim of work: To evaluate the potential role of adding green coffee extract to silymarin in improving thioacetamide induced hepatic injury in rats. Materials and methods: Thirty six albino rats were divided into 4 groups; group I (control group), group II (thioacetamide group), group III (Silymarin group) and Group IV (Silymarin and green coffee extract group). Biochemical analysis for liver function, antioxidant enzyme assay were performed. Liver sections were stained using hematoxylin and eosin, Masson trichrome stains and immunohistochemical expression for caspase-3. Morphometric and statistical analysis were done. Results: Liver functions of group II showed significant deterioration which slightly improved in group III and tend to be normal in group IV. Antioxidant enzyme levels of groupII and group III were significantly lower than that of group I; group III and group IV levels were significantly higher than that of group II. Liver sections of group II showed massive dilated congested portal vein, bile duct proliferation, and mononuclear cellular infiltrates. Group III showed slightly dilated congested portal vein in some areas while appeared normal in other areas. In group IV portal area appeared almostly normal. Marked increased collagen deposition was detected in group II, decreased in group III and markedly decreased in group IV. Immunohistochemical expression of caspase-3 was increased in group II, lower in group III and significantly low in group IV. In conclusion: Adding green coffee extract to silymarin potentiate the therapeutic effect of silymarin in improving experimentally induced hepatic injury by thioacetamide in rats.

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Section: Resultsmentioning

confidence: 99%

Effect of Silymarin versus Silymarin and Green Coffee Extract on Thioacetamide Induced Liver Injury in Adult Male Albino Rats (Histological and Immunohistochemical Study)

Abdelaal

Mousa

Ahmed

2019

Egyptian Journal of Histology

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“…A previous study has demonstrated that melatonin administration (5 mg/kg body weight per day for 1 week via i.p. injection) improved liver conditions with lower serum alanine ALT and aspartate aminotransferase (AST) levels, decreased proinflammatory cytokine expression, such as IL‐1β and TNFα, as well as profibrogenic factors such as transforming growth factor beta 1 (TGF‐β1) and collagen type I in the liver by attenuating oxidative stress during TAA‐induced liver injury in rats . Melatonin supplementation may be a promising approach to regulate cholangiocyte functions and maintain liver homeostasis.…”

Section: Functional Roles and Therapeutic Potentials Of Melatonin Andmentioning

confidence: 99%

Melatonin and circadian rhythms in liver diseases: Functional roles and potential therapies

Sato

Meng

Francis

et al. 2020

Journal of Pineal Research

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Circadian rhythms and clock gene expressions are regulated by the suprachiasmatic nucleus in the hypothalamus, and melatonin is produced in the pineal gland. Although the brain detects the light through retinas and regulates rhythms and melatonin secretion throughout the body, the liver has independent circadian rhythms and expressions as well as melatonin production. Previous studies indicate the association between circadian rhythms with various liver diseases, and disruption of rhythms or clock gene expression may promote liver steatosis, inflammation, or cancer development. It is well known that melatonin has strong antioxidant effects. Alcohol drinking or excess fatty acid accumulation produces reactive oxygen species and oxidative stress in the liver leading to liver injuries. Melatonin administration protects these oxidative stress‐induced liver damage and improves liver conditions. Recent studies have demonstrated that melatonin administration is not limited to antioxidant effects and it has various other effects contributing to the management of liver conditions. Accumulating evidence suggests that restoring circadian rhythms or expressions as well as melatonin supplementation may be promising therapeutic strategies for liver diseases. This review summarizes recent findings for the functional roles and therapeutic potentials of circadian rhythms and melatonin in liver diseases.

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“…Actually, the potential therapeutic approach for treatment of fibrosis is focused on deletion of the soluble factors released by hepatocytes, including inflammatory cytokines and ROS, which leading to the activation of hepatic stellate cells (HCSs) that are responsible of collagen secretion and fibrogenesis [ 219 ]. Moreover, in different experimental pathological conditions melatonin has been shown to decrease liver fibrosis [ 165 , 220 , 221 ].…”

Section: Melatonin Treatment In Liver Alterationsmentioning

confidence: 99%

Dietary Melatonin Supplementation Could Be a Promising Preventing/Therapeutic Approach for a Variety of Liver Diseases

et al. 2018

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In the therapeutic strategies, the role of diet is a well-established factor that can also have an important role in liver diseases. Melatonin, identified in animals, has many antioxidant properties and it was after discovered also in plants, named phytomelatonin. These substances have a positive effect during aging and in pathological conditions too. In particular, it is important to underline that the amount of melatonin produced by pineal gland in human decreases during lifetime and its reduction in blood could be related to pathological conditions in which mitochondria and oxidative stress play a pivotal role. Moreover, it has been indicated that melatonin/phytomelatonin containing foods may provide dietary melatonin, so their ingestion through balanced diets could be sufficient to confer health benefits. In this review, the classification of liver diseases and an overview of the most important aspects of melatonin/phytomelatonin, concerning the differences among their synthesis, their presence in foods and their role in health and diseases, are summarized. The findings suggest that melatonin/phytomelatonin supplementation with diet should be considered important in preventing different disease settings, in particular in liver. Currently, more studies are needed to strengthen the potential beneficial effects of melatonin/phytomelatonin in liver diseases and to better clarify the molecular mechanisms of action.

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Melatonin mitigates thioacetamide-induced hepatic fibrosis via antioxidant activity and modulation of proinflammatory cytokines and fibrogenic genes

Cited by 52 publications

References 54 publications

Effect of Silymarin versus Silymarin and Green Coffee Extract on Thioacetamide Induced Liver Injury in Adult Male Albino Rats (Histological and Immunohistochemical Study)

Effect of Silymarin versus Silymarin and Green Coffee Extract on Thioacetamide Induced Liver Injury in Adult Male Albino Rats (Histological and Immunohistochemical Study)

Melatonin and circadian rhythms in liver diseases: Functional roles and potential therapies

Dietary Melatonin Supplementation Could Be a Promising Preventing/Therapeutic Approach for a Variety of Liver Diseases

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