Melatonin modulates the functions of porcine granulosa cells via its membrane receptor MT2 in vitro

He, Yamei; Deng, Hong-Hui; Shi, Meihong; Bodinga, Bello Musa; Chen, Huali; Han, Zengsheng; Jiang, Zhongliang; Li, Qingwang

doi:10.1016/j.anireprosci.2016.07.015

Cited by 48 publications

(33 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous research reports found that melatonin exerts a protective function in mouse oocytes and porcine oocytes . Our results agree with previous studies which suggest that melatonin plays an important role in steroid hormone synthesis of GCs, however, the mechanism underlying how melatonin affects steroid hormone synthesis in GCs is unclear.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Liu

Yang

et al. 2019

Journal of Pineal Research

View full text Add to dashboard Cite

The interaction of granulosa cells (GCs) with oocytes is important to regulate follicle development. The exogenous melatonin promoting the maturation of oocytes by GCs has been approved in pig, however, the transcriptome profile and the functions of the genes regulated by melatonin in GCs have not yet to be fully characterized. In this study, we found melatonin could stimulate the synthesis of estradiol in pig GCs. The RNA‐seq was used to explore the effects of melatonin on gene expression, a total of 89 differentially expressed genes (DEGs) were identified. Gene ontology analysis showed DEGs which associated with regulation of cell proliferation, cell cycle, and anti‐apoptosis were significantly enriched. The functions of two DEGs, NOTCH2 and FILIP1L, were studied in pig GCs. The results showed that NOTCH2 inhibited the synthesis of estradiol, but FILIP1L promoted the synthesis of estradiol. Furthermore, inhibiting NOTCH2 in granulosa cells cocultured with cumulus‐oocyte‐complexes had no obvious effect on the maturation of pig oocyte, but could upregulate the cleavage rate of oocyte. We proved that FILIP1L had no effect on the maturation and cleavage of pig oocytes. Our work deepens the understanding of melatonin's effects on GCs and oocyte. The DEGs we found will be beneficial to reveal mechanisms of melatonin acting on GCs and oocytes and design the pharmacological interventions.

show abstract

Section: Discussionsupporting

confidence: 91%

“…At the same time lesser P4 concentrations during early follicular development can accelerate follicular growth . Steroid hormone synthesis of GCs is regulated by many factors, including LH, FSH, PRL, and melatonin …”

Section: Introductionmentioning

confidence: 99%

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Liu

Yang

et al. 2019

Journal of Pineal Research

View full text Add to dashboard Cite

show abstract

“…Melatonin could enhance the maturation of oocytes and the development of follicles in mammals2324252627 and fish2829. Despite the great differences between birds and mammals, melatonin may play a similar role in the maturation of oocytes and the development of follicles in birds as it does in mammals.…”

Section: Discussionmentioning

confidence: 99%

Melatonin implantation improved the egg-laying rate and quality in hens past their peak egg-laying age

Yang

Zhu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

The egg-laying rates of hens approximately 470 days of age exhibited a positive correlation to blood melatonin levels. The hens with an egg-laying rate <30%, 30~90% and ≥90% had blood melatonin levels of 5.8 ± 2.6, 74.0 ± 32.9 and 445.9 ± 115.3 ng/ml, respectively. When 10 mg of melatonin was implanted into the hens at 300, 360, 470 and 550 days of age, the egg-laying rates increased 4.63 ± 0.46%, 8.38 ± 1.45%, 4.93 ± 0.85% and 7.93 ± 0.91%, respectively, compared to that of the controls. Melatonin implantation in hens at 300–470 days of age was observed to enhance egg production and reduce the rate of appearance of sharpei eggs. Melatonin (10 mg) implanted in hens 360 days of age did not influence the blood levels of progesterone (P4) or the gene expression levels of ovarian follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), oestradiol receptor alpha (ERα), superoxide dismutase 2 (SOD2) or melatonin receptor 1 (MT1). In contrast, melatonin significantly elevated the serum oestradiol-17β (E2) content, down-regulated the gene expression of gonadotropin-inhibitory hormone receptor (GnIHR), and enhanced the expression of melatonin receptor 2 (MT2). This result indicates that the improved egg-laying rate by melatonin was the result of increased serum oestradiol and decreased ovarian GnIHR. These alterations may be mediated by MT2 activation.

show abstract

“…In addition, it was found that melatonin preserve the function of mitochondria through reducing and preventing ROS [20], as well as enhancing mitochondrial distribution activity and patterns during nuclear maturation phase [31], and preventing or reducing apoptosis level [21]. Lately, it had been stated that melatonin can ensure its valuable effects through activation of MTNR1A receptorin both granulosa cells and oocyte [31,32], and MTNR1B receptor only in cumulus cells [31,32,41].…”

Section: Discussionmentioning

confidence: 99%

Effect of melatonin and/or cysteamine on development and vitrification of buffalo embryos

El-Sokary

Raey

Mahmoud

et al. 2017

APJR

View full text Add to dashboard Cite

Melatonin modulates the functions of porcine granulosa cells via its membrane receptor MT2 in vitro

Cited by 48 publications

References 41 publications

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Melatonin implantation improved the egg-laying rate and quality in hens past their peak egg-laying age

Effect of melatonin and/or cysteamine on development and vitrification of buffalo embryos

Contact Info

Product

Resources

About