Melatonin Prevents Brain Damage and Neurocognitive Impairment Induced by Plasmodium Berghei ANKA Infection in Murine Model of Cerebral Malaria

Ataide, Brenda Jaqueline de Azevedo; Kauffmann, Nayara; Mendes, Nívia de Souza Franco; Torres, Marjorie Lujan Marques; Anjos, Larissa Medeiros dos; Passos, Adelaide da Conceição Fonseca; Moraes, Suellen Alessandra Soares de; Batista, Evander de Jesus Oliveira; Herculano, Anderson Manoel; Oliveira, Karen Renata Matos

doi:10.3389/fcimb.2020.541624

Cited by 15 publications

(13 citation statements)

References 62 publications

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“…Body weight variation and survival rates curve were evaluated throughout the experimental period. The evolution of the infection was also monitored daily by determining the parasitemia by Giemsa-stained slides obtained from mice tail blood as described by Ataide et al [ 25 ]. Animals positive to CM show symptoms as poor reflexes, roll-over, ataxia, limb paralysis, seizures and coma between 6- and 10-days post infection.…”

Section: Methodsmentioning

confidence: 99%

Euterpe oleracea fruit (Açai)-enriched diet suppresses the development of experimental cerebral malaria induced by Plasmodium berghei (ANKA) infection

Oliveira¹,

Torres²,

Kauffmann³

et al. 2022

BMC Complement Med Ther

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Background Cerebral malaria is one of the most severe complications attributed to protozoal infection by Plasmodium falciparum, gaining prominence in children mortality rates in endemic areas. This condition has a complex pathogenesis associated with behavioral, cognitive and motor sequels in humans and current antimalarial therapies have shown little effect in those aspects. Natural products with antioxidant and anti-inflammatory properties have become a valuable alternative therapeutic option in the treatment of distinct conditions. In this context, this study investigated the neuroprotective effect of Euterpe oleracea (açai) enriched diet during the development of experimental cerebral malaria induced by the inoculation of Swiss albino mice with Plasmodium berghei ANKA strain. Methods After Plasmodium infection, animals were maintained on a feeding with Euterpe oleracea enriched ration and parameters such as survival curve, parasitemia and body weight were routinely monitored. The present study has also evaluated the effect of açai-enriched diet on the blood-brain barrier leakage, histological alterations and neurocognitive impairments in mice developing cerebral malaria. Results Our results demonstrate that between 7th–19th day post infection the survival rate of the group treated with açai enriched ration was higher when compared with Plasmodium-infected mice in which 100% of mice died until the 11th days post-infection, demonstrating that açai diet has a protective effect on the survival of infected treated animals. The same was observed in the brain vascular extravasation, where Evans blue dye assays showed significantly less dye extravasation in the brains of Plasmodium-infected mice treated with açai enriched ration, demonstrating more preserved blood-brain barrier integrity. Açai-enriched diet also attenuate the histopathological alterations elicited by Plasmodium berghei infection. We also showed a decrease of the neurological impairments arising from the exposure of cerebral parenchyma in the group treated with açai diet, ameliorating motor and neuropsychiatric changes, analyzed through the SHIRPA protocol. Conclusion With these results, we conclude that the treatment with açai enriched ration decreased the mortality of infected animals, as well as protected the blood-brain barrier and the neurocognitive deficits in Plasmodium-infected animals.

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Section: Methodsmentioning

confidence: 99%

Euterpe oleracea fruit (Açai)-enriched diet suppresses the development of experimental cerebral malaria induced by Plasmodium berghei (ANKA) infection

Oliveira¹,

Torres²,

Kauffmann³

et al. 2022

BMC Complement Med Ther

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“…Mice were inoculated intraperitoneally (i.p.) with 10 6 parasitized red blood cells (pRBCs), suspended in 200 μ l of phosphate-buffered saline (PBS; pH 7.4) as previously described by Ataide et al [ 23 ]. Treatments with GSH (8 mg/kg/day diluted in 100 μ l PBS) or vitamin C (8 mg/kg/day diluted in 100 μ l PBS) were performed by i.p.…”

Section: Methodsmentioning

confidence: 99%

Differential Effect of Antioxidants Glutathione and Vitamin C on the Hepatic Injuries Induced by Plasmodium berghei ANKA Infection

Kauffmann

Penha

Braga

et al. 2021

BioMed Research International

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Malaria is a life-threatening disease caused by Plasmodium and represents one of the main public health problems in the world. Among alterations associated with the disease, we highlight the hepatic impairment resulting from the generation of oxidative stress. Studies demonstrate that liver injuries caused by Plasmodium infection are associated with unbalance of the antioxidant system in hepatocytes, although little is known about the role of antioxidant molecules such as glutathione and vitamin C in the evolution of the disease and in the liver injury. To evaluate disease complications, murine models emerge as a valuable tool due to their similarities between the infectious species for human and mice. Herein, the aim of this study is to evaluate the effect of antioxidants glutathione and vitamin C on the evolution of murine malaria and in the liver damage caused by Plasmodium berghei ANKA infection. Mice were inoculated with parasitized erythrocytes and treated with glutathione and vitamin C, separately, both at 8 mg/kg during 7 consecutive days. Our data showed that during Plasmodium infection, treatment with glutathione promoted significant decrease in the survival of infected mice, accelerating the disease severity. However, treatment with vitamin C promoted an improvement in the clinical outcomes and prolonged the survival curve of infected animals. We also showed that glutathione promoted increase in the parasitemia rate of Plasmodium-infected animals, although treatment with vitamin C has induced significant decrease in parasitemia rates. Furthermore, histological analysis and enzyme biochemical measurement showed that treatment with glutathione exacerbates liver damage while treatment with vitamin C mitigates the hepatic injury induced by the infection. In summary, the current study provided evidences that antioxidant molecules could differently modulate the outcome of malaria disease; while glutathione aggravated the disease outcome and liver injury, the treatment with vitamin C protects the liver from damage and the evolution of the condition.

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“…The brains were then removed and placed in a Petri dish to assess the presence of vascular extravasation in the animal's encephalic microvasculature, performed with the naked eye and under a magnifying glass to capture images. Quantification of extravasated dye was performed as described by Ataíde et al [30].…”

Section: Vascular Permeability Testmentioning

confidence: 99%

Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii

Borges,

de Macedo,

da Silva

et al. 2024

Preprint

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Acanthamoeba genus amoebae can affect humans with diseases such as Granulomatous Amebic Encephalitis (GAE), a highly lethal neuroinfection. Several aspects about the disease still need to be elucidated. Animal models of GAE have allowed advances in these aspects. This work tested Wistar rats (Rattus norvegicus albinus) as an animal model of GAE. For this, 32 animals were infected with 1x106 A. castellanii trophozoites, T4 genotype. Ameba recovery tests were carried out on plates of agar, vascular extravasation assays, behavioral tests and histopathological technique with H/E staining. Data were submitted to linear regression analysis, one-way ANOVA and Tukey's test, performed in the GraphPad Prism® 8.0 program, with a significance level of p

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Melatonin Prevents Brain Damage and Neurocognitive Impairment Induced by Plasmodium Berghei ANKA Infection in Murine Model of Cerebral Malaria

Cited by 15 publications

References 62 publications

Euterpe oleracea fruit (Açai)-enriched diet suppresses the development of experimental cerebral malaria induced by Plasmodium berghei (ANKA) infection

Euterpe oleracea fruit (Açai)-enriched diet suppresses the development of experimental cerebral malaria induced by Plasmodium berghei (ANKA) infection

Differential Effect of Antioxidants Glutathione and Vitamin C on the Hepatic Injuries Induced by Plasmodium berghei ANKA Infection

Experimental Granulomatous Amebic Encephalitis Caused by Acanthamoeba castellanii

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