Melatonin promotes parthenogenetic development of vitrified-warmed mouse MII oocytes, potentially by reducing oxidative stress through SIRT1

Lv, Tianyi; Cao, Beijia; Qin, Jianpeng; Wei, Yuanhang; Pan, Bo; Ye, Jiangfeng; Zhou, Guangbin

doi:10.1016/j.theriogenology.2023.06.012

Cited by 2 publications

(2 citation statements)

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“…In addition, vitrification also led to delayed progression of pronucleus formation, which was consistent with previous findings [ 12 ]. Previous researches suggested that exogenous antioxidants, such as melatonin [ 12 , 17 , 45 ] and resveratrol [ 20 , 66 , 67 ], could improve vitrified oocyte quality and developmental potential. In this study, similarly, we found that supplementation of TW-7 in oocyte warming, recovery, PA, and embryo culture medium could significantly alleviate oxidative damage caused by vitrification in oocytes and improve the development rate of PA embryos from vitrified MII oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, the straws (0.25 mL, IMV, France) were heat-softened and quickly pulled to get a length of 3 cm, with an inner diameter of about 0.10 mm and an outer diameter of about 0.15 mm. Vitrification-warming procedures were performed as described previously [ 45 ]. For vitrification, 8–10 oocytes were aspirated with OPS straws and exposed to phosphate buffer saline (PBS) containing 10% ethylene glycol and 10% dimethyl sulfoxide for 30 s. Then, the oocytes were transferred to PBS containing 15% ethylene glycol, 15% dimethyl sulfoxide, 300 g/L Ficoll, 0.5 mol/L sucrose, and 3 g/L fetal bovine albumin for 25 s. Finally, the straws containing oocytes were plunged into liquid nitrogen quickly.…”

Section: Methodsmentioning

confidence: 99%

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The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

Wei,

Pan,

Qin

et al. 2024

J Animal Sci Biotechnol

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Background Previous studies have shown that the vitrification of metaphase II (MII) oocytes significantly represses their developmental potential. Abnormally increased oxidative stress is the probable factor; however, the underlying mechanism remains unclear. The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate. Accumulating evidences implied that TW-7 was a powerful antioxidant, while its prospective application in oocyte cryopreservation has not been reported. Result Here, we found that parthenogenetic activation (PA) zygotes derived from vitrified MII oocytes showed elevated ROS level and delayed progression of pronucleus formation. Addition of 25 μmol/L TW-7 in warming, recovery, PA, and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MII oocytes, furtherly increase proteins related to histone lactylation such as LDHA, LDHB, and EP300 and finally improve histone lactylation in PA zygotes. The elevated histone lactylation facilitated the expression of minor zygotic genome activation (ZGA) genes and preimplantation embryo development. Conclusions Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse MII oocytes and found a potent and easy-obtained short peptide that could significantly rescue the decreased developmental potential of vitrified oocytes, which would potentially contribute to reproductive medicine, animal protection, and breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

Wei,

Pan,

Qin

et al. 2024

J Animal Sci Biotechnol

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Melatonin Regulates the Expression of VEGF and HOXA10 in Bovine Endometrial Epithelial Cells through the SIRT1/PI3K/AKT Pathway

Li,

Tang,

Chen

et al. 2024

Animals

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Melatonin plays a critical role in regulating embryo attachment in ruminants. While numerous studies have investigated its effects on early embryo development in vitro, the precise mechanisms by which melatonin influences the receptivity of endometrial epithelial cells in dairy cows remain unclear. The prerequisite for embryo implantation is the specific physiological condition of the endometrium that allows the embryo to implant, also known as endometrial receptivity. In addition to this, endometrial cells undergo processes such as proliferation, differentiation, and renewal, which makes the embryo more easily implanted. In this study, bovine endometrial epithelial cells were cultured and treated with melatonin, Silent Information Regulator 1 (SIRT1) inhibitor (EX527), and protein kinase B (AKT) phosphorylation inhibitor (periposine). RT-qPCR, Western blot, and immunofluorescence analysis were performed to investigate the effects of melatonin on the expression of target gene (SIRT1); cell proliferative genes, phosphatidylinositol-4,5-bisphosphate 3-Kinase (PI3K), AKT, cyclinD1, cyclinE1; and receptive genes (Leukemia Inhibitory Factor (LIF), Vascular Endothelial Growth Factor (VEGF), Homeobox Structure Gene 10 (HOXA10)). Additionally, microRNA (miRNA) mimics and inhibitors were used to transfect the cells to study the regulatory relationship between miRNA and receptive genes. Results indicated that melatonin activates the PI3K/AKT signaling pathway, upregulates cyclinD1 and cyclinE1, and promotes the proliferation of bovine endometrial epithelial cells. Melatonin also upregulated the expression of VEGF and HOXA10 and downregulated the expression of bta-miR-497 and bta-miR-27a-3p through SIRT1/PI3K/AKT signaling pathway. Further, bta-miR-497 and bta-miR-27a-3p were found to negatively regulate VEGF and HOXA10, respectively. Therefore, melatonin regulates the expression of VEGF and HOXA10 through the SIRT1/PI3K/AKT pathway and promotes the establishment of receptivity in bovine endometrial epithelial cells.

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Melatonin promotes parthenogenetic development of vitrified-warmed mouse MII oocytes, potentially by reducing oxidative stress through SIRT1

Cited by 2 publications

References 45 publications

The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

Melatonin Regulates the Expression of VEGF and HOXA10 in Bovine Endometrial Epithelial Cells through the SIRT1/PI3K/AKT Pathway

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