Melatonin protects human cells from clustered DNA damages, killing and acquisition of soft agar growth induced by X-rays or 970 MeV/n Fe ions

Das, Birajalaxmi; Bennett, Paula V.; Cutter, Noelle C.; Sutherland, John C.; Sutherland, Betsy M.

doi:10.3109/09553002.2011.560993

Cited by 14 publications

(28 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(76–78)] and fruit extracts (79, 80). In addition, there are a number of new potential cancer preventive agents that have been shown to mitigate in vitro SEBs of the space radiation cancer risk; examples include melatonin (81) and a synthetic triterpenoid, bardoxolone methyl, which protects against space radiation-induced transformation of human colon epithelial cells (82). …”

Section: Introductionmentioning

confidence: 99%

Biological effects of space radiation and development of effective countermeasures

Kennedy

2014

Life Sciences in Space Research

156

117

View full text Add to dashboard Cite

As part of a program to assess the adverse biological effects expected from astronaut exposure to space radiation, numerous different biological effects relating to astronaut health have been evaluated. There has been major focus recently on the assessment of risks related to exposure to solar particle event (SPE) radiation. The effects related to various types of space radiation exposure that have been evaluated are: gene expression changes (primarily associated with programmed cell death and extracellular matrix (ECM) remodeling), oxidative stress, gastrointestinal tract bacterial translocation and immune system activation, peripheral hematopoietic cell counts, emesis, blood coagulation, skin, behavior/fatigue (including social exploration, submaximal exercise treadmill and spontaneous locomotor activity), heart functions, alterations in biological endpoints related to astronaut vision problems (lumbar puncture/intracranial pressure, ocular ultrasound and histopathology studies), and survival, as well as long-term effects such as cancer and cataract development. A number of different countermeasures have been identified that can potentially mitigate or prevent the adverse biological effects resulting from exposure to space radiation.

show abstract

Section: Introductionmentioning

confidence: 99%

Biological effects of space radiation and development of effective countermeasures

Kennedy

2014

Life Sciences in Space Research

156

117

View full text Add to dashboard Cite

show abstract

“…The results of a recent study published by Das and colleagues [84] also emphasize the high efficacy of melatonin in abating molecular damage inflicted by ionizing radiation. In this case, melatonin (0.0-3.5 mM) was mixed in a solution of supercoiled plasmid DNA and the samples were then irradiated in the cold with either 0.15 or 25 Gy of X-rays (100 kVp) delivered by a Phillips Xray machine at a dose rate of 0.9 Gy/min.…”

Section: Melatonin As a Radioprotector: In Vitro Studiesmentioning

confidence: 86%

“…Melatonin also has a very long shelf-life and is inexpensive. To re-emphasize its high efficacy as a radioprotective agent, after a series of sophisticated tests of melatonin as a radiation protector at the Brookhaven National Laboratory in the United States, the authors of the published report concluded that melatonin ''is an excellent candidate as a countermeasure against radiation exposure'' [84].…”

Section: Discussionmentioning

confidence: 99%

“…In another aspect of the study, human 28SC monocytes were exposed to 5 Gy of X-rays with or without the addition of melatonin to the culture medium [84]. Both the exposure of cells to 2.5 or 5.0 Gy X-irradiation in the absence of melatonin led to the linear accumulation of double strand breaks.…”

Section: Melatonin As a Radioprotector: In Vitro Studiesmentioning

confidence: 99%

“…Clearly, these results prove that melatonin protects the genome from damage by ionizing photons. The data obtained led the authors [84] to suggest that Table 1 Studies in which melatonin and its metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), protected cells/animals from damage/death resulting from exposure to ionizing radiation. Both melatonin and AFMK are proven antioxidants and both were found to be radioprotective.…”

Section: Melatonin As a Radioprotector: In Vitro Studiesmentioning

confidence: 99%

See 2 more Smart Citations

Melatonin protection from chronic, low-level ionizing radiation

Reíter

Korkmaz

et al. 2012

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

Radio-Protective Effects of Melatonin on Subventricular Zone in Irradiated Rat: Decrease in Apoptosis and Upregulation of Nestin

et al. 2017

View full text Add to dashboard Cite

Neural stem cells are self-renewing, multipotent cells that can be found in subventricular (SVZ) and subgranular (SGZ) zones of the brain. These zones are susceptible to irradiation-induced apoptosis and oxidative stress. Melatonin (MLT) is a natural protector of neural cells against toxicity. The aim of this study was to evaluate the effects of MLT as a radio-protective material effective in reducing tissue lesions in the SVZ of the brain and changing local apoptotic potential in rats. Twenty-five Gray irradiation was applied on adult rat brain for this study. One hour before irradiation, 100 mg/kg/IP MLT was injected, and 6 h later, the animals were sacrificed. The antioxidant enzymes and MDA activity levels were measured post-sacrifice. Also, the expression level of Nestin and caspase 3 were studied by immunohistochemistry. Spectrophotometric analysis showed significant increases in the amount of malondialdehyde (MDA) levels in the irradiation-exposed (RAD) group compared to that of the control (Co) group (P < 0.05). Pre-treatment with MLT (100 mg/kg) ameliorates the harmful effects of the aforementioned 25 Gy irradiation by increasing antioxidant enzyme activity and decreasing MDA levels. A significant reduction in apoptotic cells was observed in rats treated with MLT 1 h before exposure (P < 0.001). Nestin-positive cells were also reduced in the RAD group (P < 0.001). Our results confirm the anti-apoptotic and antioxidant role of MLT. The MLT concentration used may serve as a threshold for significant protection against 25 Gy gamma irradiations on neural stem cells in SVZ.

show abstract

Melatonin protects human cells from clustered DNA damages, killing and acquisition of soft agar growth induced by X-rays or 970 MeV/n Fe ions

Abstract: Melatonin's effective reduction of radiation-induced critical DNA damages, cell killing, and striking decrease of transformation suggest that it is an excellent candidate as a countermeasure against radiation exposure, including radiation exposure to astronaut crews in space travel.

Cited by 14 publications

References 46 publications

Biological effects of space radiation and development of effective countermeasures

Biological effects of space radiation and development of effective countermeasures

Melatonin protection from chronic, low-level ionizing radiation

Radio-Protective Effects of Melatonin on Subventricular Zone in Irradiated Rat: Decrease in Apoptosis and Upregulation of Nestin

Contact Info

Product

Resources

About