Melatonin receptor expression in rat cerebral artery

Chucharoen, Prapapun; Chetsawang, Banthit; Srikiatkhachorn, Anan; Govitrapong, Piyarat

doi:10.1016/s0304-3940(03)00214-3

Cited by 14 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We confirmed the presence of MT 1 mRNA by two different RT-PCR techniques applying two different sets of primers and identified the amplicon by sequencing. Methodological differences, especially different numbers of amplification cycles may explain the discrepancy observed between our result and the results of Chucharoen et al (2003) who were not able to demonstrate MT 1 mRNA in the rat aorta. In contrast to the distinct expression of MT 1 receptors in the rat aorta, we failed to demonstrate the presence of MT 2 mRNA in this vessel.…”

Section: Discussioncontrasting

confidence: 99%

“…Using 125 I-MEL binding, specific binding sites were detected in rat cerebral arteries and the smooth muscle layer of caudal arteries, while in the same study, aorta, carotid, coronary, mesenteric, or renal arteries lacked binding sites (Viswanathan et al 1990). By RT-PCR, the MEL binding sites in the tail artery and the cerebral artery were unequivocally identified as being MT 1 (Ting et al 1999;Chucharoen et al 2003;Masana et al 2002). Expression of MT 2 mRNA was not demonstrated in cerebral arteries (Chucharoen et al 2003) and remains contradictory in caudal arteries (Ting et al 1999;Masana et al 2002).…”

Section: Introductionmentioning

confidence: 93%

“…While the expression of MT 1 protein had been shown (Benova et al 2009), demonstration of mRNA expression failed (Chucharoen et al 2003), and 125 I-MEL binding was not observed (Viswanathan et al 1990). Finally, while MT 2 mRNA expression was shown in human aorta (Ekmekcioglu et al 2003), a study in the rat aorta failed to detect the same (Chucharoen et al 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Presence and Localization of Melatonin Receptors in the Rat Aorta

et al. 2011

View full text Add to dashboard Cite

Melatonin is involved in blood pressure modulation in rats and humans. Some of the effects of melatonin are presumably mediated via two G-protein-coupled receptors (MT(1) and MT(2)), but the distribution of MT(1) and MT(2) in the cardiovascular system remains to be explored comprehensively. We investigated the expression of both the receptors in the rat aorta on mRNA level by RT-PCR and real time RT-PCR as well as on protein level via western blotting and immunofluorescence microscopy. We verified MT(1) mRNA expression in the rat aorta and demonstrated the absence of MT(2) mRNA in this vessel type. MT(1) receptors were confirmed also at the protein level, and surprisingly they were preferentially localized to the tunica adventitia. Since no daily changes in MT(1) mRNA expression were detected, we suppose that the circadian changes in circulating melatonin concentrations are sufficient to mediate circadian effects of melatonin in the aorta. The localization of MT(1) in the tunica adventitia suggests an influence of melatonin on vasa vasorum function and signal transduction in the aorta wall.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Presence and Localization of Melatonin Receptors in the Rat Aorta

et al. 2011

View full text Add to dashboard Cite

show abstract

“…32) The two melatonin receptor subtypes that have been identified in mammals have been designated as MT1 (1a) and MT2 (1b). 33) Decreased MT2 immunoreactivity and increased MT1 immunoreactivity have been reported in the hippocampus of AD patients. 34) Endothelial adhesion molecules, including ICAM-1, are primarily involved in inflammatory reactions.…”

Section: Discussionmentioning

confidence: 98%

The Effect of Circadian Melatonin Levels on Inflammation and Neurocognitive Functions Following Coronary Bypass Surgery

Geyik

Yıgıter

Akcali

et al. 2015

ATCS

View full text Add to dashboard Cite

Purpose: In this study, the relationship between the plasma levels of melatonin and intercellular adhesion molecule-1 (ICAM-1), which plays role in several intercellular interactions including inflammatory and immune responses, and early neurocognitive functions associated with ischaemia-reperfusion injury during open heart surgery is examined. Methods: Forty patients who were to undergo elective coronary artery bypass grafting (CABG) were divided into two groups, those who underwent their operations at 8 AM (group I; n = 20) and those who underwent their operations at 1 PM (group II; n = 20). Blood samples were collected prior to surgery (S1), when the aortic cross clamp was removed (S2) and 4 (S3) and 24 h after the surgery (S4). Neuropsychiatric assessment was conducted one day before and seven days after surgery. Results: Melatonin levels measured during and after surgery were also significantly higher in Group 1. ICAM-1 levels were significantly lower in Group 1 at S2 and S3. Significant deterioration was observed in postoperative neurocognitive function compared with preoperative functions in Group 2 more than Group 1. Conclusion: We hypothesise that the greater preservation of neurocognitive functions in the morning patients is associated with elevated melatonin levels, which reduce the damage from ischaemia-reperfusion injury.

show abstract

“…Geary et al, (1998) suggested that melatonin inhibits endothelial K+ channels to decrease flow-induced release of nitric oxide as well as block smooth muscle K+ channels to enhance vascular tone. A few recent publications indicated a vasoconstrictive effect of melatonin in cerebral, caudal and coronary arteries via activation of either melatonin 1 or melatonin 2 membrane receptors in experimental animals and humans (Laitinen et al, 1992;Viswanathan et al, 1993;Doolen et al, 1998;Regrigny et al, 1999;Ekmekcioglu et al, 2001;Vandeputte et al, 2001;Masana et al, 2002;Pogan et al, 2002;Chucharoen et al, 2003;O'Rourke et al, 2003). Regrigny et al (2001) demonstrated that melatonin deprival makes the arteriolar wall thinner and decreases distensibility of the cerebral arteriolar wall.…”

Section: Discussionmentioning

confidence: 99%

Effects Of Pınealectomy On Morphologıcal Features Of Blood Vessel In Chıcken: An Electron Mıcroscopıc And Stereologıcal Study

TURGUT¹,

Kaplan²,

ÜNAL³

et al. 2009

jecm

View full text Add to dashboard Cite

Although the effects of melatonin on vascular smooth muscle and vascular reactivity are described in last decade, the effects of melatonin on vascular tissues are still vague. The current study investigated the effects of pinealectomy on the blood vessel wall in the chicken. Fifteen chicks were divided into three groups, namely unoperated control, sham-operated and pinealectomized chicks. At the end of the experiment, a quantitative structural analysis of 15 sciatic artery samples from these groups was investigated. Effects of pinealectomy were quantified using volume fraction approach of stereological method. In this study, total cross-sectional area of the artery in pinealectomy group was significantly higher than those in unoperated control and sham-operated animals (p < 0.05). However, surgical pinealectomy procedure resulted in a significantly decreased thickness of the tunica adventitia of the vessel wall, in contrast to those of tunica media (p < 0.01). The present results indicate that pinealectomy had a vasodilator effect on the sciatic artery. In the light of current study, the structural changes in the vessel demonstrated by quantitative morphometric methods have been interpreted as a reflection of the role of melatonin on vessel wall and vascular reactivity, but this suggestion need to be validated in the human setting.

show abstract

Melatonin receptor expression in rat cerebral artery

Cited by 14 publications

References 17 publications

The Presence and Localization of Melatonin Receptors in the Rat Aorta

The Presence and Localization of Melatonin Receptors in the Rat Aorta

The Effect of Circadian Melatonin Levels on Inflammation and Neurocognitive Functions Following Coronary Bypass Surgery

Effects Of Pınealectomy On Morphologıcal Features Of Blood Vessel In Chıcken: An Electron Mıcroscopıc And Stereologıcal Study

Contact Info

Product

Resources

About