“…Protein extracts were prepared with 40 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM EDTA, 10% glycerol, 0.2% Triton X-100, and 1 × Roche Protease Inhibitor Cocktail (Roche Applied Science, Indianapolis, IN, USA), and then centrifuged at 10,000× g for 10 min at 4 • C. Aliquots of the supernatant were mixed with sample buffer (Tris-HCL, pH 6.8, 10% sodium dodecyl sulfate [SDS], 10 mM DTT, 20% glycerol, and 0.05% bromophenol blue). SDS-polyacrylamide gel electrophoresis (PAGE) and a blot assay were performed as described previously in [27]. Antibodies against Lhcb1, Lhcb4, RBCL, and ClpR1 were purchased from Agrisera (Vannas, Sweden).…”