Melatonin regulation of antioxidant enzyme gene expression

Mayo, Juan C.; Sainz, Rosa M.; Antolı́n, Isaac; Herrera, Federico; Martı́n, Vanesa; Rodríguez, Carmen

doi:10.1007/pl00012498

Cited by 274 publications

(215 citation statements)

References 32 publications

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“…Evidence of melatonin as an enhancer of antioxidant enzyme activity and expression with activation of its membrane receptors (MT1, MT2), via G inhibitory protein, supported our results [59,62]. Melatonin is a highly electroreactive molecule that acts primarily as a powerful electron donor and detoxifies electrodeficient ROS.…”

Section: Discussionsupporting

confidence: 82%

Long-term melatonin administration attenuates low-LET γ-radiation-induced lymphatic tissue injury during the reproductively active and inactive phases of Indian palm squirrels (Funambulus pennanti)

Sharma

Haldar²,

Chaube³

et al. 2010

BJR

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ABSTRACT. A comparative analysis of low linear energy transfer (LET) c-radiationinduced damage in the lymphatic tissue of a tropical seasonal breeder, Indian palm squirrel (Funambulus pennanti), during its reproductively active phase (RAP) and inactive phase (RIP) was performed with simultaneous investigation of the effects of long-term melatonin pre-treatment (100 mg/100 g body weight). A total of 120 squirrels (60 during RAP and 60 during RIP) were divided into 12 groups and sacrificed at 4, 24, 48, 72 and 168 h following 5 Gy c-radiation exposure; control groups were excluded from exposure. Total leukocyte count and absolute lymphocyte count (ALC) and melatonin only of peripheral blood, stimulation index, thiobarbituric-acid-reactive substances (TBARS) level, superoxide dismutase (SOD) activity, and the apoptotic index of spleen as analysed by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labelling (TUNEL) noted at observed time-points were significantly reduced in melatonin pre-treated groups during RAP and RIP. Long-term melatonin pretreatment mitigated radiation-induced alterations more prominently during RIP, as assessed by ALC, TBARS, SOD, TUNEL and caspase-3 activity, at some time-points. Our results demonstrate an inhibitory role of melatonin on caspase-3 activity in splenocytes during RAP and RIP following c-radiation-induced caspase-mediated apoptosis. Hence, we propose that melatonin might preserve the viability of immune cells of a seasonal breeder against background radiation, which is constantly present in the environment. The increasing number of personnel working with radiation, together with the development of nuclear weapons, stimulated an interest in the study of radiation effects on immunity. Radiation-induced injury due to free radicals and reactive oxygen intermediates (ROI) could impair specific immune responses. Besides ultraviolet (UV) radiation, a common environmental stressor that influences animals [1] and humans [2], X-rays and c-rays are readily available from natural and artificial sources [3][4][5]. The earth and all living things are constantly bombarded by the background radiation present in soil, water, earth and vegetation [6,7]; one of the major contributors to background radiation is c-radiation [8,9]. Funambulus pennanti (Indian tropical palm squirrel), a photoperiodic seasonal breeder that lives close to human populations, is exposed daily to solar radiation and UV as well as to background radiation. Immune functions of this squirrel are always under the effect of environmental and biotic factors, such as ambient temperature, food availability, shelter, rainfall, humidity and other stressful conditions, with hormonal variations in different seasons. As F. pennanti is sensitive to seasonal changes and stressors, it is a suitable model for studying high-dose cradiation effects on the immune function under seasonally recurrent stressors. Reports regarding the destructive effect of low linear energy transfer (LET) ionising rad...

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Section: Discussionsupporting

confidence: 82%

Long-term melatonin administration attenuates low-LET γ-radiation-induced lymphatic tissue injury during the reproductively active and inactive phases of Indian palm squirrels (Funambulus pennanti)

Sharma

Haldar²,

Chaube³

et al. 2010

BJR

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“…Melatonin has been reported to increase tissue levels of glutathione peroxidase [66,67], an enzyme that metabolizes glutathione (an antioxidant) to its oxidized form [68] and to increase the mrna for glutathione peroxidase [69,70] in the rat brain. the possible interaction of ubiquitin in this effect of melatonin has not been directly tested.…”

Section: Melatonin/ubiquitin Interaction In Oxidative Stressmentioning

confidence: 99%

Melatonin and ubiquitin: what’s the connection?

Vriend

Reíter

2014

Cell. Mol. Life Sci.

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“…25 Given that melatonin is an antioxidant agent either as a direct scavenger or indirectly by modulating the expression of antioxidant enzymes or glutathione content, 26 we decided to study the levels of MnSOD in melatonininduced NE-differentiated cells and compared with control, nondifferentiated cells. MnSOD but not CuZnSOD immunoreactivity increased in NE-like differentiated cells, either with MEL, cs-FBS or with db-cAMP (Fig.…”

Section: Ne-like Cells Show Higher Levels Of Mnsodmentioning

confidence: 99%

Upregulation of manganese superoxide dismutase (SOD2) is a common pathway for neuroendocrine differentiation in prostate cancer cells

Quiros

Sáinz

Hevia

et al. 2009

Intl Journal of Cancer

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Despite improvements in diagnosis of advanced prostate cancer (PCa), treatment is not efficient and 5-year survival is still low. Initially, the less abundant of cell types, neuroendocrine cells (NE), are involved in regulatory process but their physiological role is not fully understood. Among others, an increase in NE cells along with tumor progression has been commonly reported but their role in tumorigenesis or the molecular mechanisms of transdifferentiation is still a matter of debate. We have used human PCa cells (LNCaP) induced to differentiate to NE cells with several stimuli: androgen withdrawal, cyclic AMP or treatment with the antioxidant pineal hormone melatonin. PCa patients' specimens were also analyzed by western blotting and by immunocytochemistry. NE-like LNCaP cells express high levels of mitochondrial superoxide dismutase (MnSOD/SOD2) in addition to NE markers. MnSOD upregulation is mediated by NFjB transcription factor, mainly through p65 translocation into the nuclei. More importantly, overexpression of MnSOD induces the rise of NE-markers in LNCaP cells, showing that MnSOD upregulation might be instrumental for NE differentiation in PCa cells. Furthermore, MnSOD is highly expressed in advanced tumors of patients' when compared with control, nonpathological samples or with low-grade tumors, along with the presence of synaptophysin, a common NE marker. Also, fluorescence immunohistochemical analysis revealed that MnSOD colocalizes with NE markers in most of NE cells observed in PCa specimens. The present findings indicate that MnSOD is essential for NE transdifferentiation and mediates in part the differentiation process, which appears also to be critical in vivo. ' 2009 UICC

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Melatonin regulation of antioxidant enzyme gene expression

Cited by 274 publications

References 32 publications

Long-term melatonin administration attenuates low-LET γ-radiation-induced lymphatic tissue injury during the reproductively active and inactive phases of Indian palm squirrels (Funambulus pennanti)

Long-term melatonin administration attenuates low-LET γ-radiation-induced lymphatic tissue injury during the reproductively active and inactive phases of Indian palm squirrels (Funambulus pennanti)

Melatonin and ubiquitin: what’s the connection?

Upregulation of manganese superoxide dismutase (SOD2) is a common pathway for neuroendocrine differentiation in prostate cancer cells

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