Melatonin relieves diabetic complications and regenerates pancreatic beta cells by the reduction in NF-kB expression in streptozotocin induced diabetic rats

Farid, Alyaa; Moussa, Passant; Youssef, Manar; Haytham, Merna; Shamy, Ali; Safwat, Gehan

doi:10.1016/j.sjbs.2022.103313

“…Fructosamine (FTA) level was measured, by ab228558 fructosamine assay kit (Abcam, MA, USA), to detect the average glycemic level over the past three weeks [ 30 , 31 ]. Fasting blood glucose (FBG) and serum insulin were measured using ab65333 glucose assay kit (Abcam, MA, USA), and insulin ELISA kit (CSB-E05070r, CUSABIO, Texas, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Morphometric analysis, for pancreatic sections, was performed to determine the degree of improvement in each group (Table 2 ) according to Farid et al . [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Co-treatment with grape seed extract and mesenchymal stem cells in vivo regenerated beta cells of islets of Langerhans in pancreas of type I-induced diabetic rats

Farid

¹

,

Haridyy

²

,

Ashraf

³

et al. 2022

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Background Nowadays, diabetes mellitus is known as a silent killer because individual is not aware that he has the disease till the development of its complications. Many researchers have studied the use of stem cells in treatment of both types of diabetes. Mesenchymal stem cells (MSCs) hold a lot of potential for regenerative therapy. MSCs migrate and home at the damaged site, where they can aid in the repair of damaged tissues and restoring their function. Oxidative stress and inflammation represent a huge obstacle during MSCs transplantation. Therefore, the present study aimed to evaluate the role of grape seed extract (GSE) administration during MSCs transplantation in streptozotocin (STZ)-induced type I diabetes. Furthermore, testing some of GSE components [procyanidins(P)-B1 and P-C1] in conjunction with MSCs, in vivo, was performed to determine if one of them was more effective in relieving the measured attributes of diabetes more than the whole GSE. Methods Firstly, GSE was prepared from the seeds of Muscat of Alexandria grapes and characterized to identify its phytochemical components. Experimental design was composed of control group I, untreated diabetic group II, GSE (300 mg/kg)-treated diabetic group III, MSCs (2 × 106 cells/rat)-treated diabetic group IV and GSE (300 mg/kg)/MSCs (2 × 106 cells/rat)-treated diabetic group V. Type I diabetes was induced in rats by intravenous injection with 65 mg/kg of STZ. Treatment started when fasting blood glucose (FBG) level was more than 200 mg/dl; GSE oral administration started in the same day after MSCs intravenous injection and continued daily for 30 consecutive days. Results The results showed that GSE/MSCs therapy in type I-induced diabetic rats has dramatically managed homeostasis of glucose and insulin secretion; together with, improvement in levels of inflammatory markers and oxidative stress. Conclusion Co-treatment with GSE and MSCs in vivo regenerates beta cells in type I-induced diabetic rats.

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“…The pattern of STZ-induced liver injury usually shows severe pathological changes, including portal vein dilatation and congestion, wall destruction, surrounding leukocyte infiltration, and even severe cytoplasmic degeneration of hepatocytes [ 22 ]. Previous studies have shown mildly dilatated hyperemic central veins and marked hepatocyte microvesicular steatosis in liver sections of the untreated diabetes group, whereas administration of melatonin reduced inflammation and hepatocyte alignment in the liver sections [ 23 ]. Similar histological staining results were also obtained in the present study, indicating that STZ-induced liver tissue damage can be confirmed by observing the incomplete arrangement of liver cells, abnormal morphology of the portal triad (consisting of the portal vein, hepatic artery, and bile duct), loose liver tissue, widening of the hepatic sinusoidal space, and even the presence of infiltrating immune cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of Rice-Husk Silica Liquid in Streptozotocin-Induced Diabetic Mice

Chen

¹

,

Hong

²

,

Chiang

³

et al. 2022

Metabolites

2

0

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Type 2 diabetes mellitus is a complex multifactorial disease characterized by poor glucose tolerance and insulin resistance. Rice-husk silica liquid (RHSL) derived from rice husk has the ability to improve the dysfunction of pancreatic β-cells. This study aimed to confirm the potential protective effects of RHSL in streptozotocin (STZ)-induced diabetic mice. Diabetes was induced in male C57BL/6J mice by intraperitoneal administration of STZ (200 mg/kg BW). RHSL, food-grade silica liquid (FDSL), and rosiglitazone (RSG) were administered to diabetic mice for 12 weeks after successful induction of diabetes. During the experiment, fasting blood glucose, serum insulin, and organ weights were measured. The histopathology of liver tissue was evaluated by H&E staining. Western blotting was performed to assess protein expression levels. The results showed that RHSL significantly reversed the serum insulin levels and improved oral glucose tolerance test (OGTT) results (p < 0.05). In addition, liver sections of STZ-induced diabetic mice after RHSL treatment showed neatly arranged and intact hepatocytes. Furthermore, RHSL was more effective than FDSL in increasing the expression of SIRT1 and decreasing the expression of the PPAR-γ and p-NF-κB proteins. Taken together, this study demonstrated that RHSL ameliorated STZ-induced insulin resistance and liver tissue damage in C57BL/6J mice.

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“…Clinicians should pay more attention to the evaluation of the preoperative sleep quality of patients in clinical practice. Utilizing health education, improving the hospitalization environment, and using analgesic and anti-inflammatory drugs or melatonin [ 23 , 24 ], the patients sleep can be improved, to which will improve the quality of recovery, reduce postoperative pain, and accelerate postoperative rehabilitation.…”

Section: Discussionmentioning

confidence: 99%

Effects of Preoperative Sleep Disorders on Anesthesia Recovery and Postoperative Pain in Patients Undergoing Laparoscopic Gynecological Surgery under General Anesthesia

Yu

¹

,

Xiong

²

,

Lu

³

et al. 2022

Mediators of Inflammation

5

0

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Sleep disorder dramatically affects people’s physical and mental health. Here, we investigated the effect of preoperative sleep disorders on anesthesia recovery and postoperative pain in patients undergoing laparoscopic gynecological surgery under general anesthesia. 120 patients who underwent elective laparoscopic gynecological surgery under general anesthesia in Taizhou Central Hospital from November 2021 to March 2022 were included. According to the score of the Pittsburgh sleep quality index (PSQI), the participating patients were divided into four groups: control group (control group), mild sleep disorder group A (group A), moderate sleep disorder group B (group B), and severe sleep disorder group C (group C), with 30 patients in each group. The changes of mean arterial pressure (MAP) and heart rate (HR) at different time points, operation time, anesthesia time, extubation time, the time when Aldrete score reached 10 points, visual analog score (VAS) serum interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) were compared among different groups. Our study demonstrated that there were no significant differences in MAP and HR among the four groups at the same time points (all P > 0.05 ). Significant differences in the time of extubation and Aldrete score reaching 10 points had been found among the four groups (all P < 0.001 ), indicating more sleep disorder induced longer extubation and recovery time. There were significant differences in VAS scores among the four groups at both different and the same time points (all P < 0.001 ), suggesting more sleep disorders induced more pain in the sufferers. Serum IL-6 levels were significantly higher in the three sleep disorder groups than the control group at 6 h and 24 h after the operation (all P < 0.05 ), while group C has the highest IL-6 levels as compared to the other group ( P = 0.09 and P < 0.001 , respectively). At 6 h after operation, serum levels of TNF-α in group C were significantly higher than in the control group ( P = 0.044 ), but no significant differences were found in the other two groups (all P > 0.05 ). Positive correlation with preoperative PSQI score has been found with the times of extubation, the time of Aldrete score reaching 10 points, the VAS at 1 h, 6 h, and 24 h after operation, the level of serum IL-6 at 1 day before operation and 6 h and 24 h after operation, and the TNF-α at 6 h and 24 h after operation (all P < 0.001 ). The present study showed that the degree of preoperative sleep disorders could affect the quality of postoperative awakening and pain of patients undergoing laparoscopic gynecological surgery under general anesthesia, which might be associated with the aggravation of inflammatory reactions in the body.

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Melatonin relieves diabetic complications and regenerates pancreatic beta cells by the reduction in NF-kB expression in streptozotocin induced diabetic rats

Cited by 27 publications

References 80 publications

Co-treatment with grape seed extract and mesenchymal stem cells in vivo regenerated beta cells of islets of Langerhans in pancreas of type I-induced diabetic rats

Co-treatment with grape seed extract and mesenchymal stem cells in vivo regenerated beta cells of islets of Langerhans in pancreas of type I-induced diabetic rats

Effects of Rice-Husk Silica Liquid in Streptozotocin-Induced Diabetic Mice

Effects of Preoperative Sleep Disorders on Anesthesia Recovery and Postoperative Pain in Patients Undergoing Laparoscopic Gynecological Surgery under General Anesthesia

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