The connection between two fundamental processes in cells, the stress response and circadian timekeeping, were analyzed on Leydig cells from stressed rats. Stress increased glucocorticoids and decreased testosterone blood level. Transcriptional analysis revealed different sensitivity to stress events depending on the circadian time: the majority of steroidogenesis-related genes (Lhcgr, Nr3c1, Cyp11a1, Cyp17a1, Hsd3b1/2) were down-regulated by stress in the inactive (light) phase, but in the active (dark) phase of the day, they were unchanged or even up-regulated. Stress potentiates the expression of clock elements Bmal1/BMAL1, Per1/2/PER1, and Rev-erba. Glucocorticoid-treated rats showed a similar response, likewise stress on the negative clock regulators (Rev-erba/b, Cry1/2, Per1/2), suggesting that stress-induced glucocorticoids regulate clock loops but also outside genes through E-/RORE-box. Blockade of intratesticular glucocorticoid receptors prevented stress-induced change in expression negative clock regulators. The results reveal glucocorticoid-mediated communication between the stress- and circadian-system resets the Leydig cell's clock.