Melatonin Reverses 10-Hydroxycamptothecin-Induced Apoptosis and Autophagy in Mouse Oocyte

Wang, Lining; Zhang, Jingwen; Zhao, Chengtian; Jia, Zhenzhen; Feng, Xisheng

doi:10.1007/s43032-020-00359-4

Cited by 12 publications

(10 citation statements)

References 46 publications

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“…However, this does not exclude some beneficial effects of melatonin, when present, regarding the protection against oxidative and other forms of cell stress. This assumption would be in line with findings on the protection of oocytes by melatonin [31][32][33][34][35], which was also described when present during in vitro maturation [36][37][38][39]. From a biological point of view, these results are not surprising, because the developing oocyte lives in the melatonin-containing environment of the follicle fluid, into which this compound is especially released by the oocyte and granulosa cells [36,[40][41][42][43].…”

Section: Pluripotent and Unipotent Stem Cells: Distinction Between Pr...supporting

confidence: 87%

Melatonin and the Programming of Stem Cells

Hardeland

2022

IJMS

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Melatonin interacts with various types of stem cells, in multiple ways that comprise stimulation of proliferation, maintenance of stemness and self-renewal, protection of survival, and programming toward functionally different cell lineages. These various properties are frequently intertwined but may not be always jointly present. Melatonin typically stimulates proliferation and transition to the mature cell type. For all sufficiently studied stem or progenitor cells, melatonin’s signaling pathways leading to expression of respective morphogenetic factors are discussed. The focus of this article will be laid on the aspect of programming, particularly in pluripotent cells. This is especially but not exclusively the case in neural stem cells (NSCs) and mesenchymal stem cells (MSCs). Concerning developmental bifurcations, decisions are not exclusively made by melatonin alone. In MSCs, melatonin promotes adipogenesis in a Wnt (Wingless-Integration-1)-independent mode, but chondrogenesis and osteogenesis Wnt-dependently. Melatonin upregulates Wnt, but not in the adipogenic lineage. This decision seems to depend on microenvironment and epigenetic memory. The decision for chondrogenesis instead of osteogenesis, both being Wnt-dependent, seems to involve fibroblast growth factor receptor 3. Stem cell-specific differences in melatonin and Wnt receptors, and contributions of transcription factors and noncoding RNAs are outlined, as well as possibilities and the medical importance of re-programming for transdifferentiation.

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Section: Pluripotent and Unipotent Stem Cells: Distinction Between Pr...supporting

confidence: 87%

Melatonin and the Programming of Stem Cells

Hardeland

2022

IJMS

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“…CHOP can trigger the intrinsic apoptotic pathway through the inhibition of BCL2, BCL-XL, and MCL-1 and the upregulation of BIM, which regulates BAX-BAK-mediated mitochondrial outer membrane permeabilization, leading to Cyto C release and the Caspase cascade ( Hu et al, 2018 ; Kim and Kim, 2018 ). Previous studies have identified that the expression of key regulators of oocyte apoptosis (Bax and Caspase 3) is increased in mouse oocyte apoptosis ( Escobar et al, 2019 ; Wang F. et al, 2020 ; Wang L. et al, 2020 ). Overexpression of Bax modulates the mitochondrial ΔΨ m and triggers Cyto C release and induces the activation of Caspase 3, leading to DNA fragmentation and thereby oocyte apoptosis ( Chaube et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes

Gao

Zhang

et al. 2021

Front. Cell Dev. Biol.

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The endoplasmic reticulum (ER) is a multifunctional organelle in the cytoplasm that plays important roles in female mammalian reproduction. The endoplasmic reticulum and mitochondria interact to maintain the normal function of cells by maintaining intracellular calcium homeostasis. As proven by previous research, glycine (Gly) can regulate the intracellular free calcium concentration ([Ca2+]i) and enhance mitochondrial function to improve oocyte maturation in vitro. The effect of Gly on ER function during oocyte in vitro maturation (IVM) is not clear. In this study, we induced an ER stress model with thapsigargin (TG) to explore whether Gly can reverse the ER stress induced by TG treatment and whether it is associated with calcium regulation. The results showed that the addition of Gly could improve the decrease in the average cumulus diameter, the first polar body excretion rate caused by TG-induced ER stress, the cleavage rate and the blastocyst rate. Gly supplementation could reduce the ER stress induced by TG by significantly improving the ER levels and significantly downregulating the expression of genes related to ER stress (Xbp1, ATF4, and ATF6). Moreover, Gly also significantly alleviated the increase in reactive oxygen species (ROS) levels and the decrease in mitochondrial membrane potential (ΔΨ m) to improve mitochondrial function in porcine oocytes exposed to TG. Furthermore, Gly reduced the [Ca2+]i and mitochondrial Ca2+ ([Ca2+]m) levels and restored the ER Ca2+ ([Ca2+]ER) levels in TG-exposed porcine oocytes. Moreover, we found that the increase in [Ca2+]i may be caused by changes in the distribution and expression of inositol 1,4,5-triphosphate receptor (IP3R1) and voltage-dependent anion channel 1 (VDAC1), while Gly can restore the distribution and expression of IP3R1 and VDAC1 to normal levels. Apoptosis-related indexes (Caspase 3 activity and Annexin-V) and gene expression Bax, Cyto C, and Caspase 3) were significantly increased in the TG group, but they could be restored by adding Gly. Our results suggest that Gly can ameliorate ER stress and apoptosis in TG-exposed porcine oocytes and can further enhance the developmental potential of porcine oocytes in vitro.

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“… 90 , 91 Functioning as an antioxidant, melatonin and its derivatives protects cells from oxidation, inflammation, and apoptosis. 92 …”

Section: Therapeutics Involving the Sirt Signaling Pathway To Improve...mentioning

confidence: 99%

“… 101 The addition of 1 mM melatonin decreased ROS levels, spindle abnormalities, and DNA breakage ratio in mice oocytes affected by 10‐Hydroxycamptothecin. 92 Similarly, in vivo supplementation of melatonin suppresses mitochondrial dysfunction, reversing oocyte meiotic deteriorations in mice. 103 Another data identified melatonin treatment delayed postovulatory mouse oocyte aging via a SIRT1‐MnSOD‐dependent pathway, suggesting molecular mechanisms support the further application of melatonin in infertility treatment.…”

Section: Therapeutics Involving the Sirt Signaling Pathway To Improve...mentioning

confidence: 99%

Improvement of oocyte quality through the SIRT signaling pathway

Sato

Kawamura

2023

Reprod Medicine & Biology

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Background Oocyte quality is one of the major deciding factors in female fertility competence. Methods PubMed database was searched for reviews by using the following keyword “oocyte quality” AND “Sirtuins”. The methodological quality of each literature review was assessed using the Preferred Reporting Items for Systematic reviews and Meta‐Analyses (PRISMA) 2020 statement. Main Findings Oxidative stress has been recognized as the mechanism attenuating oocyte quality. Increasing evidence from animal experiments and clinical studies has confirmed the protective roles of the sirtuin family in improving oocyte quality via an antioxidant effect. Conclusion The protective roles in the oocyte quality of the sirtuin family have been increasingly recognized.

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Melatonin Reverses 10-Hydroxycamptothecin-Induced Apoptosis and Autophagy in Mouse Oocyte

Cited by 12 publications

References 46 publications

Melatonin and the Programming of Stem Cells

Melatonin and the Programming of Stem Cells

Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes

Improvement of oocyte quality through the SIRT signaling pathway

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