Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos

An, Quanli; Peng, Wei; Cheng, Yulin; Lu, Zhenzhen; Zhou, Chuan; Zhang, Yong; Su, Jianmin

doi:10.1002/jcp.28357

Cited by 95 publications

(61 citation statements)

References 54 publications

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“…Because melatonin reduces ROS and oxidative stress via its direct antioxidative functions, it improves cellular integrity ( Figure 3). However, indirect effects of melatonin via membrane receptors (MT1, MT2) and/or nuclear receptors (RORα) are also important to understand the processes by which melatonin alters the level of oxidative stress [56,57]. Figure 3 summarizes the mechanism of melatonin that are assumed to improve oocyte maturation and quality based on reports to date.…”

Section: Oocyte Maturation Embryo Development and Melatoninmentioning

confidence: 99%

Importance of Melatonin in Assisted Reproductive Technology and Ovarian Aging

Tamura

Jozaki

Tanabe

et al. 2020

IJMS

162

105

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Melatonin is probably produced in all cells but is only secreted by the pineal gland. The pineal secretion of melatonin is determined by the light-dark cycle, and it is only released at night. Melatonin regulates biological rhythms via its receptors located in the suprachiasmatic nuclei of the hypothalamus. Melatonin also has strong antioxidant activities to scavenge free radicals such as reactive oxygen species (ROS). The direct free radical scavenging actions are receptor independent. ROS play an important role in reproductive function including in the ovulatory process. However, excessive ROS can also have an adverse effect on oocytes because of oxidative stress, thereby causing infertility. It is becoming clear that melatonin is located in the ovarian follicular fluid and in the oocytes themselves, which protects these cells from oxidative damage as well as having other beneficial actions in oocyte maturation, fertilization, and embryo development. Trials on humans have investigated the improvement of outcomes of assisted reproductive technology (ART), such as in vitro fertilization and embryo transfer (IVF-ET), by way of administering melatonin to patients suffering from infertility. In addition, clinical research has examined melatonin as an anti-aging molecule via its antioxidative actions, and its relationship with the aging diseases, e.g., Alzheimer's and Parkinson's disease, is also underway. Melatonin may also reduce ovarian aging, which is a major issue in assisted reproductive technology. This review explains the relationship between melatonin and human reproductive function, as well as the clinical applications expected to improve the outcomes of assisted reproductive technology such as IVF, while also discussing possibilities for melatonin in preventing ovarian aging.

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Section: Oocyte Maturation Embryo Development and Melatoninmentioning

confidence: 99%

Importance of Melatonin in Assisted Reproductive Technology and Ovarian Aging

Tamura

Jozaki

Tanabe

et al. 2020

IJMS

162

105

View full text Add to dashboard Cite

show abstract

“…Up to now, electrofusion was the most commonly used method for most SCNT studies 8,9,18,19 . Regarding the piezo microinjection method, researchers were concerned about the risk of mercury contamination during the injection using micropipettes 20 .…”

Section: Discussionmentioning

confidence: 99%

“…Melatonin is an antioxidant and free-radical scavenger that may be beneficial for the production of developmentally competent embryos when electrofusion is employed 5 . Melatonin effectively reduced oxidative stress and improves the development and hatchability rates of preimplantation fertilized embryos and SCNT embryos in cows, pigs, goats, mice and other species 5,18,28,29 . The results in the present study showed that greater electrical stimulation induced higher ROS level in SCNT embryos, which were reduced by incubation with melatonin.…”

Section: Figure 1 Effects Of Electrofusion On Rabbit Scnt Embryos (A)mentioning

confidence: 99%

Melatonin Protects Rabbit Somatic Cell Nuclear Transfer (SCNT) Embryos from Electrofusion Damage

Shen

et al. 2020

Sci Rep

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The study's objectives were to examine the effects of electrofusion on rabbit somatic cell nuclear transfer (SCNT) embryos, and to test melatonin as a protective agent against electrofusion damage to SCNT embryos. The levels of reactive oxygen species (ROS), the epigenetic state (H3K9me3), and the content of endoplasmic reticulum (ER) stress-associated transcripts (IRE-1 and CHOP) were measured. Melatonin was added during the preimplantation development period. The total blastocyst cell numbers were counted, and the fragmentation rate and apoptotic index were determined and used to assess embryonic development. Electrofusion increased (1) ROS levels at the 1-, 2-, 4-, and 8-cell stages; (2) H3K9me3 levels at the 2-, 4-, and 8-cell stage; and (3) the expression of IRE-1 and CHOP at the 8-cell, 16-cell, morula, and blastocyst stages. The treatment of SCNT embryos with melatonin significantly reduced the level of ROS and H3K9me3, and the expression levels of IRE-1 and CHOP. This treatment also significantly reduced the fragmentation rate and apoptotic index of blastocysts and increased their total cell number. In conclusion, the electrofusion of rabbit SCNT embryos induced oxidative stress, disturbed the epigenetic state, and caused ER stress, while melatonin reduced this damage. Our findings are of signal importance for improving the efficiency of SCNT and for optimizing the application of electrical stimulation in other biomedical areas. open Scientific RepoRtS | (2020) 10:2186 | https://doi.

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“…The purity of isolated cortical stromal cells and theca cells was evaluated using previously described methods with minor modifications (An et al, ; Qiu et al, ; Wang et al, ). Briefly, cortical stromal cells and theca cells were fixed with Immunol Staining Fix Solution (Beyotime Institute of Biotechnology) for 20 min and then were permeabilized with 0.2% Triton X‐100 for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Evidence that downregulation of Wilms’ tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

Meng

Wang

et al. 2019

Molecular Reproduction Devel

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Bovine theca cells are thought to differentiate from cortical stromal cells, and ovary‐derived Wilms’ tumor 1+ (WT1+) cells are the primary source of mouse theca cells. However, it is not known whether the differentiation of cortical stromal cells is regulated by WT1. Here, we identified WT1 in the cortical stroma and theca layer of the bovine ovary and analyzed the theca cell functional markers in cortical stromal cells and theca cells; in addition, we determined the effects of this gene on the secretion of androstenedione and progesterone by cortical stromal cells and the responsiveness of cortical stromal cells to luteinizing hormone (LH) in vitro. We used quantitative reverse‐transcription polymerase chain reaction (RT‐qPCR), western blot analysis, and immunohistochemistry to discover that the cortical stroma had higher WT1 expression than the theca layer. We used RT‐qPCR and ELISA analyses to determine that the cortical stromal cells had lower levels of androstenedione and progesterone secretion and LHR messenger RNA expression than the levels of the theca cells. In cultured bovine cortical stromal cells, we found that WT1 downregulation increased androstenedione and progesterone secretion but had no effect on the LH responsiveness. Notably, the increase in androstenedione and progesterone secretion was associated with an increase in 3‐β‐hydroxysteroid dehydrogenase expression. In conclusion, the results suggest that WT1 is involved in the differentiation of cortical stromal cells into cells with characteristics similar to theca cells of antral follicles in adult bovine ovaries.

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Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos

Cited by 95 publications

References 54 publications

Importance of Melatonin in Assisted Reproductive Technology and Ovarian Aging

Importance of Melatonin in Assisted Reproductive Technology and Ovarian Aging

Melatonin Protects Rabbit Somatic Cell Nuclear Transfer (SCNT) Embryos from Electrofusion Damage

Evidence that downregulation of Wilms’ tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

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