2016
DOI: 10.1111/jpi.12365
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Melatonin suppresses TPA‐induced metastasis by downregulating matrix metalloproteinase‐9 expression through JNK/SP‐1 signaling in nasopharyngeal carcinoma

Abstract: Nasopharyngeal carcinoma (NPC), a disease common in the South-East Asian population, has high lymph node metastatic ability. Melatonin, an endogenously produced substance present in animals, plants, fungi, and bacteria, has oncostatic activity via several mechanisms. The molecular mechanisms involved in melatonin-mediated tumor inhibitory potential are not completely defined. Here, we show that melatonin treatment inhibits TPA-induced cell motility by regulating the matrix metalloproteinase-9 (MMP-9) expressio… Show more

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Cited by 98 publications
(105 citation statements)
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References 67 publications
(110 reference statements)
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“…5 cells/ well) in 24-well plates and then treated with PSH (0, 20, 40 and 80 μM) for 24 h. The cultured medium was collected after treatment, mixed with a loading buffer and subjected to 0.1% gelatin-8% SDS-PAGE electrophoresis. After electrophoresis were completed, the gels were washed 30 minutes twice in 2.5% Triton X-100 then incubated in reaction buffer (40 mM Tris-HCl, pH 8.0, 0.02% NaN 3 , 10 mM CaCl 2 ) at 37°C for 20 h. Finally, the gel was stained with Coomassie brilliant blue R-250 for visualization as previously described [24]. Bands corresponding to proteolytic activity of MMP-2 were evaluated by ImageJ software.…”
Section: Cell Viability (Mtt Assay)mentioning
confidence: 99%
“…5 cells/ well) in 24-well plates and then treated with PSH (0, 20, 40 and 80 μM) for 24 h. The cultured medium was collected after treatment, mixed with a loading buffer and subjected to 0.1% gelatin-8% SDS-PAGE electrophoresis. After electrophoresis were completed, the gels were washed 30 minutes twice in 2.5% Triton X-100 then incubated in reaction buffer (40 mM Tris-HCl, pH 8.0, 0.02% NaN 3 , 10 mM CaCl 2 ) at 37°C for 20 h. Finally, the gel was stained with Coomassie brilliant blue R-250 for visualization as previously described [24]. Bands corresponding to proteolytic activity of MMP-2 were evaluated by ImageJ software.…”
Section: Cell Viability (Mtt Assay)mentioning
confidence: 99%
“…No. G1272) at 37 °C in an atmosphere of 5% CO 2 [20]. To inhibit mitochondrial fission, Mdivi1 was used for 2 hours.…”
Section: Cell Culturementioning
confidence: 99%
“…Cell migration and invasion were assayed according to the methods described by Ho et al (Ho et al, 2016). After treatment with RIE for 24 h, the surviving HONE-1, NPC-39 and NPC-BM cells were harvested and seeded to a Boyden chamber (Neuro Probe, Cabin John, MD, USA) at 10 4 cells per well in serum-free medium, and then incubated for 24 h at 37 C. To determine cell migration, the cells were seeded into the Boyden chamber on membrane filters that were not coated with Matrigel as described previously (Ho et al, 2016).…”
Section: Cell Migration and Invasion Assaysmentioning
confidence: 99%
“…Unlike other head and neck cancers, the mainstay therapy for NPC is external beam radiotherapy to the nasopharynx and neck bilaterally, because the tumor cells are sensitive to radiation and the incidence of lymph node metastasis is noticeably high, even in the early stages of the disease (Su et al, 2011). Although the prognosis of NPC is considered to be relatively good among all head and neck cancers, treatment failure in the form of regional failure due to lymph node metastasis is common for patients following definitive therapy (Ng et al, 2011;Ho et al, 2016). Therefore, new agents capable of preventing or inhibiting metastasis of NPC cells are needed to improve the therapeutic outcomes of this disease.…”
Section: Introductionmentioning
confidence: 99%