Melatonin synchronizes protein synthesis rhythm in hepatocyte cultures as an agonist of intercellular calcium and protein kinases

Brodsky, V. Ya.; Голиченков, В. А.; Zvezdina, N. D.; Fateeva, V. I.; Мальченко, Л. А.

doi:10.1134/s1062360409030084

Cited by 4 publications

(8 citation statements)

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“…Like the signal factors we studied previously, it is a calcium agonist [12]. Blockade of changes in cytoplasmic calcium with BAPTA-AM [2] leads to liquidation of protein synthesis rhythm, initiated by melatonin in the control. Other drugs, modulating the concentration of calcium ions in cells and stimulating protein kinase activities, can be potential signal factors involved in protein synthesis rhythm organization.…”

Section: Resultsmentioning

confidence: 97%

“…The study was carried out on primary cultures of rat hepatocytes [1,2,7,8]. Solid cultures with cells lying close to each other were used.…”

Section: Methodsmentioning

confidence: 99%

“…Melatonin is used in cardiology and gastroenterology, as an antistress factor, and as a means for normalization of sleep duration and quality [4]. The chain of processes leading to synchronization of protein synthesis by melatonin includes its modifi cation of the cytoplasmic calcium [2], stage 1 of the signal factor work.…”

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confidence: 99%

“…One of the indicators of cell-cell interactions and synchronization of the cell population is circahoralian rhythm of protein synthesis in cell cultures. One of the studied signal molecules and rhythm synchronizers is melatonin, effectively synchronizing the protein synthesis rhythm being used in nanomolar doses [1,2]. Melatonin is used in cardiology and gastroenterology, as an antistress factor, and as a means for normalization of sleep duration and quality [4].…”

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confidence: 99%

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Melatonin Modifies the Rhythm of Protein Synthesis

Brodsky

Dubovaya

Zvezdina³

et al. 2010

Bull Exp Biol Med

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Melatonin (5 nM) added to medium with primary hepatocyte cultures shifted the phase of circahoralian rhythm of protein synthesis and hence, can be a factor synchronizing fluctuations in protein synthesis and rhythm organizer in the hepatocyte population. Blockade of melatonin receptors with luzindole (20 nM) arrested rhythm organization of protein synthesis by melatonin. Prospects of studying biochemical mechanisms of protein synthesis rhythm organization with other drugs (calcium agonists, similarly to melatonin) are discussed.

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Section: Resultsmentioning

confidence: 97%

“…The study was carried out on primary cultures of rat hepatocytes [1,2,7,8]. Solid cultures with cells lying close to each other were used.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Melatonin Modifies the Rhythm of Protein Synthesis

Brodsky

Dubovaya

Zvezdina³

et al. 2010

Bull Exp Biol Med

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“…A general population rhythm of protein synthesis has been observed in dense hepatocyte and kerati nocyte cultures (for recent data, see Brodsky et al, 2006Brodsky et al, , 2009Brodsky et al, , 2011. This rhythm is expressed soon after medium replacement, which indicates that fluctua tions in the level of protein synthesis in the cells become self synchronized as a result of direct cell interactions.…”

Section: Introductionmentioning

confidence: 90%

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

et al. 2012

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A comparative study was performed of dense 5 hour cultures of rat hepatocytes and equal density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen coated class slides in serum free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium che lator BAPTA AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchro nization in MSC consists in calcium dependent phosphorylation of cell proteins.

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Melatonin as the most effective organizer of the rhythm of protein synthesis in hepatocytes in vitro and in vivo

Brodsky

Zvezdina

2010

Cell Biology International

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Recent data has extended a large array of melatonin functions by the discovery of melatonin's involvement in the organization and regulation of the rhythm of intracellular protein synthesis. An ultradian rhythm in total protein synthesis has been detected in primary hepatocyte cultures 5 min after addition of 1-5 nM melatonin to the medium. The melatonin effect was mediated via its receptors (as shown in experiments with luzindole), leading to the cell synchronization as well as the mean rate of protein synthesis rate being increased. The chain of processes synchronizing the oscillation of the rate protein synthesis throughout the hepatocyte population includes Ca²+ fluxes {experiments with BAPTA-AM [1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetomethyl ester)]}. Inhibition of protein kinase activity (experiments with H7) inhibited the synchronizing function of melatonin. Activation of protein kinase activity results in a shift of the protein synthesis oscillation; the effect was the same as melatonin added to the culture medium. In another series of experiments, after melatonin was intraperitoneally injected to rat (0.015-0.020 μg/kg), hepatocytes were isolated and cultures established. A synchronizing effect of melatonin in vivo was detected as early as in the estimates from the direct action of melatonin on cell cultures. In the cultures obtained from old rats provided with melatonin, the amplitude of protein synthesis rhythm was enhanced, i.e. cell-cell interactions were increased, as well as rate of the protein synthesis being enhanced.

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Melatonin synchronizes protein synthesis rhythm in hepatocyte cultures as an agonist of intercellular calcium and protein kinases

Cited by 4 publications

References 5 publications

Melatonin Modifies the Rhythm of Protein Synthesis

Melatonin Modifies the Rhythm of Protein Synthesis

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

Melatonin as the most effective organizer of the rhythm of protein synthesis in hepatocytes in vitro and in vivo

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