Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder

Erşahin, Mehmet; Ozdemır, Zarıfe Nıgar; Özsavcı, Derya; Akakın, Dilek; Yeğen, Berrak Ç.; Reíter, Russel J.; Şener, Göksel

doi:10.1111/j.1600-079x.2011.00948.x

Cited by 30 publications

(18 citation statements)

References 55 publications

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“…It was reported that, in animal models of SCI, melatonin has been found highly effective in reducing the level of lipid peroxidation following injury, and the effects are attributed to its antioxidative actions. 20,21 Furthermore, our results are also in agreement with the study of Samantaray et al 1 who demonstrated that melatonin significantly decreased caspase-3 activity in SCI animals. Melatonin prevents cell damage and death.…”

Section: Discussionsupporting

confidence: 92%

“…It is well known that the formation of MDA, an end product of lipid peroxidation, is a major indicator of oxidative injury. 1,5,20 In the current study, SCI caused significant elevations in MDA levels in spinal cord tissues demonstrating oxidative stress, while caspase-3 activity was also increased. It was reported that, in animal models of SCI, melatonin has been found highly effective in reducing the level of lipid peroxidation following injury, and the effects are attributed to its antioxidative actions.…”

Section: Discussionsupporting

confidence: 51%

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The effect of melatonin on spinal cord after ischemia in rats

et al. 2015

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Study design: Experimental animal model to assess ischemic spinal cord injury (SCI) following occlusion of the thoracoabdominal aorta. Objectives: In the present study, we aimed to investigate the role of melatonin on SCI induced by ischemia and following reperfusion. Setting: Animal Research Laboratory, Inonu University, Malatya, Turkey. Methods: We evaluated oxidative damage and caspase-3 activity. In total, 32 adult Wistar albino rats were divided into four groups: Group 1, control (n = 8); Group 2 (n = 8), those subjected to ischemia/reperfusion (IR) by clamping the thoraco-abdominal aorta; Group 3 (n = 8), melatonin (50 mg kg −1 ) treated; and Group 4 (n = 8), melatonin (50 mg kg −1 ) followed by ischemia. All animals were kept alive for 48 h, and then spinal cord samples were removed. We assayed oxidative damage by measuring malondialdehyde (MDA), apoptosis by measuring activated caspase-3 (using immunoblots) and intrinsic antioxidative capacity by measuring reduced glutathione (GSH) levels in the spinal cord. Results: The results indicated a significant decrease in activity of caspase-3 in SCI animals after treatment with melatonin, as it significantly decreased the formation of MDA and decelerated the loss of GSH. Conclusion: This study suggested that melatonin could be an effective neuroprotective agent for treatment of SCI.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 51%

The effect of melatonin on spinal cord after ischemia in rats

et al. 2015

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“…It functions as a signalling molecule . In a previous study, we demonstrated that SCI reduced NGF levels in bladder tissue in which contractility of bladder strips was impaired . There are limited published data regarding NGF in penile tissues or its relationship with ED.…”

Section: Discussionmentioning

confidence: 92%

Melatonin and tadalafil treatment improves erectile dysfunction after spinal cord injury in rats

Tavukçu

Şener

Tınay

et al. 2014

Clin Exp Pharma Physio

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Oxidative stress plays an important role both in spinal cord injury (SCI) and erectile dysfunction (ED). The present study investigated the effects of melatonin and tadalafil treatment alone or in combination on SCI-induced ED. Male Wistar albino rats (n = 40) were divided into five groups: sham-operated control and SCI-injured rats given either vehicle, melatonin (10 mg/kg, i.p.), tadalafil (10 mg/kg, p.o.) or a combination of melatonin and tadalafil. Spinal cord injury was induced using a standard weight-drop method. On Day 7 after SCI, intracavernosal pressure (ICP) was measured and all rats were decapitated. Cavernosal tissues were obtained to examine caspase 3, nitric oxide synthase (NOS), myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, as well as cGMP, nerve growth factor (NGF), malondialdehyde (MDA) and glutathione (GSH) levels. Spinal cord injury caused oxidative damage, as evidenced by increases in MDA and cGMP levels. In addition, MPO and caspase 3 activites were increased after SCI, whereas GSH and NGF levels and SOD activity were reduced. Melatonin effectively reversed these oxidative changes. Furthermore, in rats treated with both melatonin and tadalafil, the recoveries were more pronounced than in rats given either melatonin or tadalafil alone. The ICP/mean arterial pressure value in vehicle-treated SCI rats was significantly higher than in the control group, whereas in the tadalafil- and tadalafil + melatonin-treated groups have returned this value had returned to control levels. As an individual treatment, and especially when combined with tadalafil, a well-known agent in the treatment of ED, melatonin prevented SCI-induced oxidative damage to cavernosal tissues and restored ED, most likely due to its anti-oxidant effects.

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“…Melatonin and its metabolic derivatives are very effective, direct, free radical scavengers and also stimulate activities of several antioxidative enzymes. Oxidative stress induced by spinal cord injury (SCI) affects multiple organ systems, and melatonin has gained recent popularity as an antioxidant treatment in the nervous system . However, the effects of melatonin on promoting differentiation of neural stem cells remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Combination of melatonin and Wnt‐4 promotes neural cell differentiation in bovine amniotic epithelial cells and recovery from spinal cord injury

Gao

Bai

Zheng

et al. 2016

Journal of Pineal Research

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Although melatonin has been shown to exhibit a wide variety of biological functions, its effects on promoting differentiation of neural cells remain unknown. Wnt signaling mediates major developmental processes during embryogenesis and regulates maintenance, self-renewal, and differentiation of adult mammalian stem cells. However, the role of the noncanonical Wnt pathway during neurogenesis remains poorly understood. In this study, the amniotic epithelial cells ( AECs) were isolated from bovine amnion and incubated with various melatonin concentrations (0.01, 0.1, 1, 10, or 100 μm) and 5 × 10(-5) m all-trans retinoic acid (RA) for screening optimum culture medium of neural differentiation, compared with each groups, 1 μm melatonin and 5 × 10(-5) m RA were selected to induce neural differentiation of AECs, and then siMT1, siMT2, oWnt-4, and siWnt-4 were expressed in AECs to research role of these genes in neural differentiation. Efficiency of neural differentiation was evaluated after expressed above genes using flow cytometry. Cell function of neural cells was demonstrated in vivo using spinal cord injury model after cell transplantation, and damage repair of spinal cord was assessed using cell tracking and Basso, Beattie, Bresnahan Locomotor Rating Scale scores. Results demonstrated that melatonin stimulated melatonin receptor 1, which subsequently increased bovine amniotic epithelial cell vitality and promoted differentiation into neural cells. This took place through cooperation with Wnt-4. Additionally, following cotreatment with melatonin and Wnt-4, neurogenesis gene expression was significantly altered. Furthermore, single inhibition of melatonin receptor 1 or Wnt-4 expression decreased expression of neurogenesis-related genes, and bovine amniotic epithelial cell-derived neural cells were successfully colonized into injured spinal cord, which suggested participation in tissue repair.

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Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder

Cited by 30 publications

References 55 publications

The effect of melatonin on spinal cord after ischemia in rats

The effect of melatonin on spinal cord after ischemia in rats

Melatonin and tadalafil treatment improves erectile dysfunction after spinal cord injury in rats

Combination of melatonin and Wnt‐4 promotes neural cell differentiation in bovine amniotic epithelial cells and recovery from spinal cord injury

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