Melittin induces autophagy to alleviate chronic renal failure in 5/6-nephrectomized rats and angiotensin II-induced damage in podocytes

Zhang, Yufan; Xu, Huaping; Qiao, Hongwei; Zhao, Ya; Jiang, Minmin

doi:10.4162/nrp.2024.18.2.210

Cited by 3 publications

(2 citation statements)

References 44 publications

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“…Our studies confirmed no toxicological impacts from Melittin, even at the highest concentration of 100 µg/kg tested. Furthermore, prior studies using intraperitoneal administration in mice (at doses of 0.75 and 1.5 mg/kg) and rats (at 25, 50, and 100 µg/kg) showed no significant changes in immune parameters, underlining its immunological safety [32,33]. Notably, the highest dose in rat studies, 100 µg/kg, matches the high concentration used in our research.…”

Section: Discussionsupporting

confidence: 71%

Comparison of Muscle Regeneration Effects at Different Melittin Concentrations in Rabbit Atrophied Muscle

Jang,

Kwon,

Lee

et al. 2024

IJMS

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This research aimed to explore the healing impacts of Melittin treatment on gastrocnemius muscle wasting caused by immobilization with a cast in rabbits. Twenty-four rabbits were randomly allocated to four groups. The procedures included different injections: 0.2 mL of normal saline to Group 1 (G1-NS); 4 μg/kg of Melittin to Group 2 (G2-4 μg/kg Melittin); 20 μg/kg of Melittin to Group 3 (G3-20 μg/kg Melittin); and 100 μg/kg of Melittin to Group 4 (G4-100 μg/kg Melittin). Ultrasound was used to guide the injections into the rabbits’ atrophied calf muscles following two weeks of immobilization via casting. Clinical measurements, including the length of the calf, the compound muscle action potential (CMAP) of the tibial nerve, and the gastrocnemius muscle thickness, were assessed. Additionally, cross-sectional slices of gastrocnemius muscle fibers were examined, and immunohistochemistry and Western blot analyses were performed following two weeks of therapy. The mean regenerative changes, as indicated by clinical parameters, in Group 4 were significantly more pronounced than in the other groups (p < 0.05). Furthermore, the cross-sectional area of the gastrocnemius muscle fibers and immunohistochemical indicators in Group 4 exceeded those in the remaining groups (p < 0.05). Western blot analysis also showed a more significant presence of anti-inflammatory and angiogenic cytokines in Group 4 compared to the others (p < 0.05). Melittin therapy at a higher dosage can more efficiently activate regeneration in atrophied gastrocnemius muscle compared to lower doses of Melittin or normal saline.

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Section: Discussionsupporting

confidence: 71%

Comparison of Muscle Regeneration Effects at Different Melittin Concentrations in Rabbit Atrophied Muscle

Jang,

Kwon,

Lee

et al. 2024

IJMS

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“…Renal tubular atrophy and interstitial fibrosis were evaluated by Masson’s trichrome staining using a Masson staining kit, and semiquantitative analysis was performed according to the percentage of collagen area stained in the visual field [ 26 ]. For periodic acid-Schiff (PAS) staining, renal tissue slices were incubated with a periodic acid solution for 5 min and sequentially with Schiff reagent for 30 min at 37 °C in the dark after being washed with ddH 2 O and then counterstained with hematoxylin [ 27 ]. We used PAS staining to observe the mesangial matrix and basement membrane and evaluate glomerular sclerosis.…”

Section: Methodsmentioning

confidence: 99%

Astragaloside IV relieves passive heymann nephritis and podocyte injury by suppressing the TRAF6/NF-κb axis

Ma,

Hu,

Ruan

et al. 2024

Renal Failure

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The pathogenesis of membranous nephropathy (MN) involves podocyte injury that is attributed to inflammatory responses induced by local immune deposits. Astragaloside IV (AS-IV) is known for its robust anti-inflammatory properties. Here, we investigated the effects of AS-IV on passive Heymann nephritis (PHN) rats and TNF-α-induced podocytes to determine the underlying molecular mechanisms of MN. Serum biochemical parameters, 24-h urine protein excretion and renal histopathology were evaluated in PHN and control rats. The expression of tumor necrosis factor receptor associated factor 6 (TRAF6), the phosphorylation of nuclear factor kappa B (p-NF-κB), the expression of associated proinflammatory cytokines (TNF-α, IL-6 and IL-1β) and the ubiquitination of TRAF6 were measured in PHN rats and TNF-α-induced podocytes. We detected a marked increase in mRNA expression of TNF-α, IL-6 and IL-1β and in the protein abundance of p-NF-κB and TRAF6 within the renal tissues of PHN rats and TNF-α-induced podocytes. Conversely, there was a reduction in the K48-linked ubiquitination of TRAF6. Additionally, AS-IV was effective in ameliorating serum creatinine, proteinuria, and renal histopathology in PHN rats. This effect was concomitant with the suppression of NF-κB pathway activation and decreased expression of TNF-α, IL-6, IL-1β and TRAF6. AS-IV decreased TRAF6 levels by promoting K48-linked ubiquitin conjugation to TRAF6, which triggered ubiquitin-mediated degradation. In summary, AS-IV averted renal impairment in PHN rats and TNF-α-induced podocytes, likely by modulating the inflammatory response through the TRAF6/NF-κB axis. Targeting TRAF6 holds therapeutic promise for managing MN.

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Melittin treatment suppressed malignant NSCLC progression through enhancing CTSB-mediated hyperautophagy

Wang,

Yuan,

et al. 2024

Biomedicine & Pharmacotherapy

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Melittin induces autophagy to alleviate chronic renal failure in 5/6-nephrectomized rats and angiotensin II-induced damage in podocytes

Cited by 3 publications

References 44 publications

Comparison of Muscle Regeneration Effects at Different Melittin Concentrations in Rabbit Atrophied Muscle

Comparison of Muscle Regeneration Effects at Different Melittin Concentrations in Rabbit Atrophied Muscle

Astragaloside IV relieves passive heymann nephritis and podocyte injury by suppressing the TRAF6/NF-κb axis

Melittin treatment suppressed malignant NSCLC progression through enhancing CTSB-mediated hyperautophagy

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