Meloidogyne incognita genes involved in the repellent behavior in response to ascr#9

Abstract

Meloidogyne incognita is one of the globally serious plant parasitic nematodes. New control measure is urgently needed to replace the common chemical control method. Ascarosides are pheromones regulating the nematodes’ aggregation, avoidance, mating, dispersal and dauer recovery and formation. Ascr#9, one of the ascarosides, exhibits the potential to repel M. incognita . However, the nematode genes involved in the perception of ascr# 9 signal are totally unknown. In this study, the transcriptome of ascr#9-treated second stage M. incognita juveniles (J2s) was analyzed, 44 pathways were significantly affected, multiple ligand-receptor and mucin type O-glycan were induced, and olfactory transduction was disturbed. A total of 11 highly differentially expressed genes involved in neuroactive ligand-receptor interaction and FMRFamide-like peptide related process were identified and knocked down by RNAi. The dispersal rates of M. incognita with three knocked-down genes ( flp-14 , mgl-1 and ADOR-1 ) significantly decreased, respectively, when ascr#9 was present. The results demonstrate that flp-14 , mgl-1 , and ADOR-1 are involved in the dispersal behavior of M. incognita nematodes responding to ascr#9, which promotes the interaction study between ascarosides and M. incognita , and provides new ideas for the prevention and control of M. incognita by using pheromone ascarosides. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-76370-5.