Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa

Aprilyani, Aprilyani; Supramana, Supramana; Suastika, Gede

doi:10.14692/jfi.11.5.143

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…incognita, M. javanica, M. arenaria, M. hapla, M. fallax dan M. chitwoodi merupakan nematoda yang merugikan secara ekonomi pada pertanaman kentang (Adam et al 2007). Tiga spesies Meloidogyne ditemukan menginfeksi pertanaman kentang di Pulau Jawa, yaitu M. javanica, M. incognita dan M. arenaria (Aprilyani et al 2015). NPA secara morfologi sangat mirip satu dengan lain dan beberapa spesies nematoda puru akar sering ditemukan bersamaan menginfeksi tanaman.…”

Section: Pendahuluanunclassified

“…Runutan (Aprilyani et al 2015), sedangkan pada tanaman wortel di Pulau Jawa dan Gowa, Sulawesi Selatan telah berhasil diidentifikasi M. incognita, M. javanica, M. arenaria, dan M. hapla (Taher et al 2012;Hikmia et al 2012;Halimah et al 2013;Mirsam et al 2015). Berdasarkan hasil wawancara di lokasi pengambilan sampel, bibit kentang yang ditanam di Sulawesi Utara berasal dari Pengalengan, Jawa Barat.…”

Section: Spesies Meloidogyneunclassified

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Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara

Utami

Supramana

Giyanto

2017

J Fitopatol Indones

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ABSTRAKNematoda puru akar (Meloidogyne spp.) adalah salah satu penyebab menurunnya produksi kentang di Sulawesi Utara. Hingga saat ini spesies Meloidogyne pada kentang di Sulawesi Utara belum diidentifikasi. Penelitian ini bertujuan mendeteksi dan mengidentifikasi spesies Meloidogyne pada kentang dan mengetahui hubungan kekerabatannya dengan spesies dari negara lain. Sampel umbi kentang bergejala bintil dikumpulkan dari 3 sentra produksi kentang di Provinsi Sulawesi Utara, yaitu Kakenturan (Minahasa Selatan), Purworejo, dan Singsingon (Bolaang Mongondow Timur). Identifikasi morfologi dilakukan berdasarkan pola perineal nematoda betina. Identifikasi molekuler dengan teknik PCR menggunakan primer spesifik untuk mengamplifikasi daerah ITS-rDNA, dilanjutkan dengan sikuensing fragmen DNA dan analisis filogenetika. Dua spesies Meloidogyne berhasil diidentifikasi, yaitu M. javanica (sampel asal Kakenturan, Purworejo, Singsingon), dan M. incognita (sampel asal Purworejo). M. javanica dan M. incognita sampel asal Sulawesi Utara memiliki tingkat homologi berturut-turut hingga 97.5% dan 100 % dengan spesies yang sama asal Cina.Kata kunci: homologi, identifikasi molekuler, ITS-rDNA, morfologi ABSTRACT Root-knot nematode (Meloidogyne spp.). is one of the main constraint of potato production in North Sulawesi. Little is known about Meloidogyne species infecting potatoes in North Sulawesi. Therefore, research was conducted to identify Meloidogyne spp. on potatoes in North Sulawesi and further study their relationship with related species from other countries. Infected potato tubers with pimple-like knot symptom were collected from three potato production centers, i.e. Kakenturan (South Minahasa), Purworejo, and Singsingon (East Bolaang Mongondow). Morphological identification was conducted based on the perineal pattern of the female; whereas molecular identification was conducted by PCR using specific primer for ITS-rDNA, followed by DNA sequencing and phylogenetic analysis. Two Meloidogyne species were identified i.e. M. javanica (samples form Kakenturan, Purworejo and Singsingon) and M. incognita (samples from Purworejo). M. javanica and M. incognita from North Sulawesi are similar to the related species from China with homology level of 97.5 % and 100 %, respectively.

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Section: Pendahuluanunclassified

Section: Spesies Meloidogyneunclassified

Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara

Utami

Supramana

Giyanto

2017

J Fitopatol Indones

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“…The presence of PPNs on potato plants in Indonesia has been documented in some prior studies; these studies indicated that major potato-growing regions across the country, including Wonosobo, Batu City, and several locations in West Java, were all likely to be infected by nematodes (Aprilyani et al 2015;Syafii et al 2018). However, in Indonesia, the diversity of PPNs that infect potato plants and their diversity at various altitudes still needs further study.…”

Section: Introductionmentioning

confidence: 95%

Diversity of plant-parasitic nematodes in potato fields at different altitudes in Probolinggo District, East Java, Indonesia

2022

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Abstract. Prada AP, Pratiwi GNC, Wagiyana. 2022. Diversity of plant-parasitic nematodes in potato fields at different altitudes in Probolinggo District, East Java, Indonesia. Cell Biol Dev 6: 61-67. Plant parasitic nematodes (PPNs) are microorganisms sensitive to environmental conditions. Therefore, plant parasitic nematode diversity in a field may affect the decision of effective management tactics. This study aimed to assess the diversity of plant parasitic nematodes in potato fields at three different altitudes. The study was carried out in Probolinggo District, East Java, Indonesia. The sampling area was divided into three groups based on altitude variations, namely 1,008 m asl., 1,413 m asl., and 1,875 m asl. Soil samples were collected randomly in a zigzag pattern. Samples were collected from 20 points across each field at a depth of 20-30 cm below the soil surface. Each sub-sample included up to 500 g of soil. The white head tray method extracted nematodes from 500 g of soil from each subsample. After characterizing the extracted nematodes, the absolute density and dominance values were determined. The results revealed variations in the three areas with different altitudes. The total number of plant-parasitic nematodes (PPN) discovered on the field at an altitude of 1,008 m asl. was 57.00 ± 16.43. Furthermore, at an altitude of 1,413 m asl., the total number of PPNs discovered is 41.67 ± 10.98. The total number of nematodes on the field at an altitude of 1,875 m asl. was 50.50 ± 12.60. The findings of this study also indicated that Meloidogyne sp. was the genus with the highest population on the field, at an altitude of 1,008 m asl. On the other hand, Pratylenchus sp. is the most abundant nematode in the field between 1,413 and 1,875 m asl. An identical situation also occurred with the nematode dominance values in the three fields studied.

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Major Constraints to Potato Production in Indonesia: a Review

Taylor¹,

Dawson²

2021

Am. J. Potato Res.

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Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa

Cited by 4 publications

References 5 publications

Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara

Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara

Diversity of plant-parasitic nematodes in potato fields at different altitudes in Probolinggo District, East Java, Indonesia

Major Constraints to Potato Production in Indonesia: a Review

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