Melon juice concentrate supplementation in an animal model of obesity: Involvement of relaxin and fatty acid pathways

Carillon, Julie; Saby, Marion; Barial, Sandy; Sansone, Anna; Scanferlato, Roberta; Gayrard, Nathalie; Lajoix, Anne‐Dominique; Jover, Bernard; Chatgilialoglu, Chryssostomos; Ferreri, Carla

doi:10.1016/j.jff.2019.05.027

Cited by 1 publication

(1 citation statement)

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“…The work-up of the blood samples followed the described procedures [23][24][25]. In brief, each whole blood sample (100 µL) in EDTA was added to PBS (0.5 mL) and centrifuged at 5000× g for 10 min at 4 • C for 5 consecutive cycles to eliminate any traces of plasma.…”

Section: Lipid Analysismentioning

confidence: 99%

Erythrocyte Plasma Membrane Lipid Composition Mirrors That of Neurons and Glial Cells in Murine Experimental In Vitro and In Vivo Inflammation

Stanzani

Sansone

Brenna

et al. 2023

Cells

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Lipid membrane turnover and myelin repair play a central role in diseases and lesions of the central nervous system (CNS). The aim of the present study was to analyze lipid composition changes due to inflammatory conditions. We measured the fatty acid (FA) composition in erythrocytes (RBCs) and spinal cord tissue (gas chromatography) derived from mice affected by experimental allergic encephalomyelitis (EAE) in acute and remission phases; cholesterol membrane content (Filipin) and GM1 membrane assembly (CT-B) in EAE mouse RBCs, and in cultured neurons, oligodendroglial cells and macrophages exposed to inflammatory challenges. During the EAE acute phase, the RBC membrane showed a reduction in polyunsaturated FAs (PUFAs) and an increase in saturated FAs (SFAs) and the omega-6/omega-3 ratios, followed by a restoration to control levels in the remission phase in parallel with an increase in monounsaturated fatty acid residues. A decrease in PUFAs was also shown in the spinal cord. CT-B staining decreased and Filipin staining increased in RBCs during acute EAE, as well as in cultured macrophages, neurons and oligodendrocyte precursor cells exposed to inflammatory challenges. This regulation in lipid content suggests an increased cell membrane rigidity during the inflammatory phase of EAE and supports the investigation of peripheral cell membrane lipids as possible biomarkers for CNS lipid membrane concentration and assembly.

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Section: Lipid Analysismentioning

confidence: 99%