Melt Electrospinning and Its Technologization in Tissue Engineering

Muerza-Cascante, Maria Lourdes; Haylock, David N.; Hutmacher, Dietmar W.; Dalton, Paul D.

doi:10.1089/ten.teb.2014.0347

Cited by 186 publications

(140 citation statements)

References 96 publications

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“…This 3D printing technique directly writes sub-and low-micron homogenous fibres with a controlled scaffold architectures, such as porosity, pore size and interconnectivity [46]. These properties facilitate cell functions and infiltration into the scaffold, which are directly linked to ECM production and deposition [23].…”

Section: Discussionmentioning

confidence: 99%

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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Section: Discussionmentioning

confidence: 99%

“…architectures [21], with fibre dimensions down to the cell size [22], in a solvent-free process, representing an ideal platform for applications in bone tissue engineering [23]. Melt electrospun written scaffolds made of medical grade poly(ɛ-caprolactone) (PCL) have supported de novo formation of bone in vivo [24,25].…”

Section: Introductionmentioning

confidence: 99%

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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“…Electrospinning has been used for diverse tissue engineering applications in research laboratories [32][33][34][35][36][37][38][39][40][41][42][43][44][45]. The technique spins highly charged polymer solutions into nanofibers or microfibers.…”

Section: Fabrication Of Fibrous Scaffolds Using Solution and Melt Elementioning

confidence: 99%

Commercializing Electrospun Scaffolds For Pluripotent Stem Cell-Based Tissue Engineering Applications

Mohtaram¹,

Karamzadeh

Shafieyan³

et al. 2017

Electrospinning

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Tissue engineering, the process of combining bioactive scaffolds often with cells to produce replacements for damaged organs, represents an enormous market opportunity. This review critically evaluates the commercialization potential of electrospun scaffolds for applications in stem cell biology, including tissue engineering. First, it provides an overview of pluripotent stem cells (PSCs) and their defining properties, pluripotency and the ability to self-renew. These cells serve as an important tool for engineering tissues, including for clinical applications. Next, we review the technique of electrospinning and its promise for fabricating cell culture substrates and scaffolds for directing tissue formation from stem cells and compare these scaffolds to existing technologies, such as hydrogels. We address the associated market for electrospun scaffolds for PSCs and its potential for growth along with highlighting the importance of 3D cell culture substrates for PSCs by analyzing the net capital invested in this market and the associated growth rate. This review finishes by detailing the current state of commercializing electrospun scaffolds along with pathways for translating these scaffolds from research laboratories into successful start-up companies and the associated challenges with this process.

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“…Fused Deposition Modelling (FDM) is one the most common additive manufacturing techniques and is a leading technology in the rapidly growing commercial 3D printing market (Hern, 2014 (Berner et al, 2013;Jensen et al, 2015), total disc replacement (van Uden, Silva-Correia, Correlo, Oliveira, & Reis, 2015), osteochondral defects (Schumann, Ekaputra, Lam, & Hutmacher, 2007;Swieszkowski, Tuan, Kurzydlowski, & Hutmacher, 2007) and cranial defects (Castilho et al, 2014;Jensen et al, 2014;Rohner, Hutmacher, Cheng, Oberholzer, & Hammer, 2003 Dalton, 2015). This observation has led to the development of advanced techniques such as electrospinning which combines the high-precision and rapid printing capability of FDM with much finer and more precise fabrication resolution (MuerzaCascante et al, 2015).…”

Section: Fused Deposition Modellingmentioning

confidence: 99%

“…Melt-electrospinning has been used more favourably over solution electrospinning in biofabrication because melt-electrospinning does not require cytotoxic solutions (Dalton, Joergensen, Groll, & Moeller, 2008). Also, one benefit of melt-electrospinning over other extrusion-based techniques such as FDM is its ability to produce substantially finer polymer fibres, ranging from approximately 270nm to 500µm in diameter (Muerza-Cascante et al, 2015). This ultimately improves fibre attachment and cell proliferation throughout the scaffold.…”

Section: Electrospinningmentioning

confidence: 99%

[leave for Adam - copyright supporting evidence]Designing patient-specific melt-electrospun scaffolds for bone regeneration

Paxton¹

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Biofabrication, or the 3D printing of biological-relevant tissues, is revolutionising our ability to treat patients who have suffered tissue loss as a result of trauma, disease or birth defect. As a subset of the Tissue Engineering field, biofabrication research is focussing on optimising the fabrication of implantable constructs, known as scaffolds, which provide a support structure for cell infiltration and growth, ultimately dissolving and restoring tissue, completely healing the patient.While research has focused on developing the mechanical capability to print structures using 3D printing, alongside biological advances to create highly biocompatible, bioactive constructs which have enhanced regenerative properties, less research has focused on developing methods of designing scaffolds which are anatomically matched to individual patients.In this thesis, a novel method for designing patient-specific scaffold for bone regeneration, to be fabricating using the melt-electrospinning 3D printing technique, was developed. The method was then applied to three clinically-relevant case studies, examining how to accurately design scaffolds to treat a wide range of orthopaedic injuries. Medical scan data was obtained from two patients and a third defect was recreated from an anatomical skull model. Following data acquisition, scaffolds were designed using 3D modelling software and processed into slices. These slices were processed by a proprietary g-code generation program which automatically generates the required computer instructions to fabricate each of the suitable layers using a meltelectrospinning machine. A skull scaffold to treat a large cranial defect, a femur scaffold to fill a void after a realignment procedure and a patella scaffold to improve the external shape of the reconstructed bone were successfully designed. The computer instructions were then trialled on the melt-electrospinning machine to assess the success of the generated g-code.In collaboration with the Biofabrication and Tissue Morphology group at the Queensland University of Technology, as well as the Orthopaedic Unit at the Royal Brisbane Hospital, this research project has successfully demonstrated the ability to fabricate patient-specific scaffolds, which one day could be used clinically to treat patients suffering from bone loss.

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Melt Electrospinning and Its Technologization in Tissue Engineering

Cited by 186 publications

References 96 publications

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

Commercializing Electrospun Scaffolds For Pluripotent Stem Cell-Based Tissue Engineering Applications

[leave for Adam - copyright supporting evidence]Designing patient-specific melt-electrospun scaffolds for bone regeneration

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