Members of the Kunitz-type protease inhibitor gene family of potato inhibit soluble tuber invertase in vitro

Glaczinski, Heike; Heibges, Andreas; Salamini, R.; Gebhardt, Christiane

doi:10.1007/bf02736112

Cited by 33 publications

(27 citation statements)

References 36 publications

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“…Functional genomics approaches revealed that InvInhs and inhibitors of pectin methylesterase (PMEI) belong to the same protein family, which is referred to as PMEI-related proteins (PMEI-RP) (Hothorn et al 2004). In addition, some members of the kunitz-type protease inhibitor gene family have also been characterized as putative invertase inhibitors in potato (Glaczinski et al 2002). However, the expression of one putative kunitz-type invertase inhibitor gene (AY594178) in potato had minor effect on acid invertase activities and RS accumulation in our laboratory (data unpublished).…”

Section: Introductionmentioning

confidence: 75%

Cloning and molecular characterization of putative invertase inhibitor genes and their possible contributions to cold-induced sweetening of potato tubers

et al. 2010

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Invertase inhibitors (InvInh) interacted with invertases (Inv) and inhibited their activities involved in reducing sugars (RS) accumulation in cold-stored potato tubers. Understanding their potential contribution to RS accumulation is of both theoretical and practical importance because RS accumulation is a costly postharvest problem for both potato producers and processors. In this study, four genes with significant sequence homology to NtInvInhs were identified from potato and their possible contributions to cold-induced sweetening (CIS) of tubers were investigated together with StInv1, an acid invertase gene previously clarified corresponsive to CIS. Transcripts analysis of these StInvInhs and StInv1 among six potato genotypes with distinct CIS sensitivity indicated that StInvInh2 had a negative power regression to RS increase of the cold-stored tubers while a positive linear regression was obtained with StInv1. The relative expression ratio calculated by StInv1/StInvInh2 performed a very significant correlation to RS accumulation, suggesting a possible interaction between StInv1 and StInvInh2 in response to CIS. The bimolecular fluorescence complementation visualized the interaction between StInv1 and StInvInh2A and with StInvInh2B in both onion epidermal cells and tobacco BY-2 cells and demonstrated that these two inhibitors may be the isoforms of StInvInh2 as the counterparts of StInv1. The recombinant StInvInh2B protein inhibited the activities of soluble acid invertase indicating evidently its inhibitory properties. Our results strongly suggest that the interaction between StInv1 and StInvInh2 may play critical roles in controlling the CIS through posttranslational regulation of StInv1 by StInvInh2 in potato tubers and will provide novel tools and resources for improving CIS tolerance of potatoes.

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Section: Introductionmentioning

confidence: 75%

Cloning and molecular characterization of putative invertase inhibitor genes and their possible contributions to cold-induced sweetening of potato tubers

et al. 2010

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“…4a, b). KTIs are frequently induced after pest and pathogen attack and act primarily as inhibitors of exogenous proteinases 16 ; therefore the expansion of the KTI family may provide resistance to biotic stress for the newly evolved vulnerable underground organ.…”

Section: Tuber Biologymentioning

confidence: 99%

Genome sequence and analysis of the tuber crop potato

Xu¹,

S²,

Cheng³

et al. 2011

Nature

1,807

1,083

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Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and is central to global food security. It is clonally propagated, highly heterozygous, autotetraploid, and suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone to sequence and assemble 86% of the 844-megabase genome. We predict 39,031 protein-coding genes and present evidence for at least two genome duplication events indicative of a palaeopolyploid origin. As the first genome sequence of an asterid, the potato genome reveals 2,642 genes specific to this large angiosperm clade. We also sequenced a heterozygous diploid clone and show that gene presence/absence variants and other potentially deleterious mutations occur frequently and are a likely cause of inbreeding depression. Gene family expansion, tissue-specific expression and recruitment of genes to new pathways contributed to the evolution of tuber development. The potato genome sequence provides a platform for genetic improvement of this vital crop.

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“…No electronic northern expression data are available on this sequence, but the observed proteomic variation could be inversely correlated with acid b-fructofuranosidase (spots 6, 57, 73, 74, 75, 115, 144, and 145) expression, which reaches a maximum during the maturation period. The Kunitz-type invertase inhibitor plays a role in plant defense mechanisms against pests and diseases, but, recently, a Kunitz-type inhibitor from potato (Solanum tuberosum) tuber was found to inhibit soluble invertase (Glaczinski et al, 2002). Its role in sugar metabolism in tomato fruit might deserve more in-depth investigation.…”

Section: Young Fruit Metabolismmentioning

confidence: 99%

Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening

Faurobert

Mihr²,

Bertin³

et al. 2007

Plant Physiol.

226

195

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Tomato (Solanum lycopersicum) is a model plant for studying fleshy fruit development. Several genetic and molecular approaches have been developed to increase our knowledge about the physiological basis of fruit growth, but very few data are yet available at the proteomic level. The main stages of fruit development were first determined through the dynamics of fruit diameter and pericarp cell number. Then, total proteins were extracted from pericarp tissue at six relevant developmental stages and separated by two-dimensional gel electrophoresis. Protein patterns were markedly different between stages.Proteins showing major variations were monitored. We identified 90 of 1,791 well-resolved spots either by matrix-assisted laser-desorption ionization time-of-flight peptide mass fingerprinting or liquid chromatography-mass spectrometry sequencing and expressed sequence tag database searching. Clustered correlation analysis results pointed out groups of proteins with similar expression profiles during fruit development. In young fruit, spots linked to amino acid metabolism or protein synthesis were mainly expressed during the cell division stage and down-regulated later. Some spots linked to cell division processes could be identified. During the cell expansion phase, spots linked to photosynthesis and proteins linked to cell wall formation transiently increased. In contrast, the major part of the spots related to C compounds and carbohydrate metabolism or oxidative processes were up-regulated during fruit development, showing an increase in spot intensity during development and maximal abundance in mature fruit. This was also the case for spots linked to stress responses and fruit senescence. We discuss protein variations, taking into account their potential role during fruit growth and comparing our results with already known variations at mRNA and metabolite-profiling levels.

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Members of the Kunitz-type protease inhibitor gene family of potato inhibit soluble tuber invertase in vitro

Cited by 33 publications

References 36 publications

Cloning and molecular characterization of putative invertase inhibitor genes and their possible contributions to cold-induced sweetening of potato tubers

Cloning and molecular characterization of putative invertase inhibitor genes and their possible contributions to cold-induced sweetening of potato tubers

Genome sequence and analysis of the tuber crop potato

Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening

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