Membrane and cytoplasmic location of streptolysin S precursor

Calandra, Gary B.; Cole, Roger M.

doi:10.1128/iai.31.1.386-390.1981

“…It utilises mechanisms for extracellular survival and multiplication in body £uids and tissue spaces. The pathogenicity of this organism is primarily achieved by its ability to produce a number of potential virulence factors such as streptolysin O, streptolysin S [12], pyrogenic toxins and a variety of enzymes. The major virulence factor is the surface M-protein, which allows the bacteria to resist phagocytic engulfment [2].…”

Section: Discussionmentioning

confidence: 99%

Atypical behaviour and survival of Streptococcus pyogenes L forms during intraperitoneal infection in rats

Michailova

¹

2000

FEMS Immunology and Medical Microbiology

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Groups of rats were injected intraperitoneally with cell wall-deficient (L) forms of Streptococcus pyogenes, with their parental (S) forms, as well as with a combined inoculum of both forms (S+L). Peritoneal exudate samples were harvested on days 1, 3, 7, 15 and 30 after challenge and were investigated by microbiological, electron microscopic, cytometric and biochemical methods. Parental S forms were isolated from peritoneal exudate samples up to day 15 post infection, while L form cultures were isolated until the end of the examined interval. Electron microscopic examination revealed continuous adhesion of L forms on the macrophage surface as well as intracellular persistence inside them. It was demonstrated that the intraperitoneal inflammatory response to L form infection was higher than to the other infections and the monocyte-macrophage populations were predominant. The established atypical behaviour and long survival of S. pyogenes L forms in the rat's peritoneum could explain some of the mechanisms of the pathogens' persistence as well as the reasons for chronic streptococcal infections.

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“…It utilises mechanisms for extracellular survival and multiplication in body fluids and tissue spaces. The pathogenicity of this organism is primarily achieved by its ability to produce a number of potential virulence factors such as streptolysin O, streptolysin S [12], pyrogenic toxins and a variety of enzymes. The major virulence factor is the surface M‐protein, which allows the bacteria to resist phagocytic engulfment [2].…”

Section: Discussionmentioning

confidence: 99%

Atypical behaviour and survival ofStreptococcus pyogenesL forms during intraperitoneal infection in rats

Michailova

¹

,

Маркова

²

,

Radoucheva

³

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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Groups of rats were injected intraperitoneally with cell wall-deficient (L) forms of Streptococcus pyogenes, with their parental (S) forms, as well as with a combined inoculum of both forms (S+L). Peritoneal exudate samples were harvested on days 1, 3, 7, 15 and 30 after challenge and were investigated by microbiological, electron microscopic, cytometric and biochemical methods. Parental S forms were isolated from peritoneal exudate samples up to day 15 post infection, while L form cultures were isolated until the end of the examined interval. Electron microscopic examination revealed continuous adhesion of L forms on the macrophage surface as well as intracellular persistence inside them. It was demonstrated that the intraperitoneal inflammatory response to L form infection was higher than to the other infections and the monocyte-macrophage populations were predominant. The established atypical behaviour and long survival of S. pyogenes L forms in the rat's peritoneum could explain some of the mechanisms of the pathogens' persistence as well as the reasons for chronic streptococcal infections.

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“…We have utilized a relatively new muralysin, mutanolysin (8), to disrupt a number of different groups of streptococci (1,2). The enzyme, produced by Streptomyces globisporus, is an Nacetyl muramidase (9) and is active against most gram-positive organisms except Staphylococcus spp.…”

mentioning

confidence: 99%