Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication

Ratajczak, Janina; Wysoczynski, Marcin; Hayek, Fadi; Janowska‐Wieczorek, Anna; Ratajczak, Mariusz Z.

doi:10.1038/sj.leu.2404296

Cited by 1,225 publications

(1,140 citation statements)

References 89 publications

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“…RNA-proteolipid complexes/microvesicles/exosomes have been isolated from sera of cancer patients [78,79] and from cancer cell lines [80,81]. The importance of communication between tumor cells and their environment through fusion of shed membrane microvesicles to neighboring cells was discussed by Ratajczak et al [82]. These microvesicles, 30-100 nm in diameter [83], can be shed from the cell surface or via inward budding of endosome membranes [84].…”

Section: Origin Of Extracellular Nucleic Acidsmentioning

confidence: 99%

Relevance of circulating tumor cells, extracellular nucleic acids, and exosomes in breast cancer

Friel

Corcoran

Crown

et al. 2010

Breast Cancer Res Treat

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Early detection of cancer is vital to improved overall survival rates. At present, evidence is accumulating for the clinical value of detecting occult tumor cells in peripheral blood, plasma, and serum specimens from cancer patients. Both molecular and cellular approaches, which differ in sensitivity and specificity, have been used for such means. Circulating tumor cells and extracellular nucleic acids have been detected within blood, plasma, and sera of cancer patients. As the presence of malignant tumors are clinically determined and/or confirmed upon biopsy procurement-which in itself may have detrimental effects in terms of stimulating cancer progression/metastases-minimally invasive methods would be highly advantageous to the diagnosis and prognosis of breast cancer and the subsequent tailoring of targeted treatments for individuals, if reliable panels of biomarkers suitable for such an approach exist. Herein, we review the current advances made in the detection of such circulating tumor cells and nucleic acids, with particular emphasis on extracellular nucleic acids, specifically extracellular mRNAs and discuss their clinical relevance.

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Section: Origin Of Extracellular Nucleic Acidsmentioning

confidence: 99%

Relevance of circulating tumor cells, extracellular nucleic acids, and exosomes in breast cancer

Friel

Corcoran

Crown

et al. 2010

Breast Cancer Res Treat

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“…In cancer progression ( Figure 3 ), C‐EVs can create a suitable microenvironment by promoting angiogenesis, regulating immunity, and remodeling surrounding tissue, or by laying the foundation for metastasis by forming a premetastatic niche 37, 38, 39, 40. It appears that cancers co‐opt EVs, a natural nanodelivery system, to transfer damaging information by altering the composition of EVs; this ability has inspired scientists to design novel drug therapeutics through reverse engineering.…”

Section: Introductionmentioning

confidence: 99%

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

2018

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Extracellular vesicles (EVs) are ubiquitous nanosized membrane vesicles consisting of a lipid bilayer enclosing proteins and nucleic acids, which are active in intercellular communications. EVs are increasingly seen as a vital component of many biological functions that were once considered to require the direct participation of stem cells. Consequently, transplantation of EVs is gradually becoming considered an alternative to stem cell transplantation due to their significant advantages, including their relatively low probability of neoplastic transformation and abnormal differentiation. However, as research has progressed, it is realized that EVs derived from native‐source cells may have various shortcomings, which can be corrected by modification and optimization. To date, attempts are made to modify or improve almost all the components of EVs, including the lipid bilayer, proteins, and nucleic acids, launching a new era of modularized EV therapy through the “modular design” of EV components. One high‐yield technique, generating EV mimetic nanovesicles, will help to make industrial production of modularized EVs a reality. These modularized EVs have highly customized “modular design” components related to biological function and targeted delivery and are proposed as a promising approach to achieve personalized and precision medicine.

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“…Moreover, ASCs have the ability to differentiate into adipocytes, myocytes, chondrocytes and osteoblasts, which underlines their potential utility in future cell‐based therapies. The pro‐regenerative properties of ASCs are explained by their paracrine and autocrine activities based on the secretion of membrane‐derived extracellular vesicles (ExMVs), which are known to play a critical role in intracellular signalling 15, 16. ExMVs were demonstrated to contain a broad range of growth factors, including vascular endothelial growth factors, fibroblast growth factors and transforming growth factor‐β—all of which are crucial in the treatment of MetS 17.…”

Section: Introductionmentioning

confidence: 99%

Combination of resveratrol and 5‐azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells

Marycz

Kornicka

Irwin‐Houston³

et al. 2018

J Cellular Molecular Medi

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Endocrine disorders have become more and more frequently diagnosed in humans and animals. In horses, equine metabolic syndrome (EMS) is characterized by insulin resistance, hyperleptinemia, hyperinsulinemia, inflammation and usually by pathological obesity. Due to an increased inflammatory response in the adipose tissue, cytophysiological properties of adipose derived stem cells (ASC) have been impaired, which strongly limits their therapeutic potential. Excessive accumulation of reactive oxygen species, mitochondria deterioration and accelerated ageing of those cells affect their multipotency and restrict the effectiveness of the differentiation process. In the present study, we have treated ASC isolated from EMS individuals with a combination of 5‐azacytydine (AZA) and resveratrol (RES) in order to reverse their aged phenotype and enhance osteogenic differentiation. Using SEM and confocal microscope, cell morphology, matrix mineralization and mitochondrial dynamics were assessed. Furthermore, we investigated the expression of osteogenic‐related genes with RT‐PCR. We also investigated the role of autophagy during differentiation and silenced PARKIN expression with siRNA. Obtained results indicated that AZA/RES significantly enhanced early osteogenesis of ASC derived from EMS animals. Increased matrix mineralization, RUNX‐2, collagen type I and osteopontin levels were noted. Furthermore, we proved that AZA/RES exerts its beneficial effects by modulating autophagy and mitochondrial dynamics through PARKIN and RUNX‐2 activity.

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Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication

Cited by 1,225 publications

References 89 publications

Relevance of circulating tumor cells, extracellular nucleic acids, and exosomes in breast cancer

Relevance of circulating tumor cells, extracellular nucleic acids, and exosomes in breast cancer

Modularized Extracellular Vesicles: The Dawn of Prospective Personalized and Precision Medicine

Combination of resveratrol and 5‐azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells

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